Trizol reagent (Life Technologies Corporation, Carlsbad, CA, USA) was used to extract total RNA of HepG2 cells based on the manufacturer’s instructions. The cDNA synthesis from 2 μg of total RNA was performed by using cDNA Synthesis SuperMix kit (TaKaRa, Dalian, China). The relative expression of ANRIL was determined by using SYBR-Green Real-Time Master Mix (Toyobo, Co., Ltd., Osaka, Japan). The relative expression of miR-191 was examined by using the Taqman MicroRNA Reverse Transcription Kit and Taqman Universal Master Mix II with the TaqMan MicroRNA Assay (Applied Biosystems). The qRT-PCR was performed by using 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA). Data were calculated by 2−ΔΔCt method, and normalized to β-actin and U6, respectively.
Cdna synthesis supermix kit
Manufactured by Takara Bio
Sourced in China
The CDNA Synthesis SuperMix kit is a reagent designed for the reverse transcription of RNA to complementary DNA (cDNA). It contains all the necessary components for efficient cDNA synthesis, including a reverse transcriptase enzyme, reaction buffer, and dNTPs.
Automatically generated - may contain errors
Lab products found in correlation
Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Sybr green realtime master mix, by Toyobo (1 mentions)
7300 real time pcr system, by Thermo Fisher Scientific (1 mentions)
Taqman universal master mix 2, by Thermo Fisher Scientific (1 mentions)
Taqman microrna assay, by Thermo Fisher Scientific (1 mentions)
Sybr green real time master mix, by Takara Bio (1 mentions)
Mirna qpcr assay kit, by Thermo Fisher Scientific (1 mentions)
7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)
2 protocols using cdna synthesis supermix kit
1
Quantification of ANRIL and miR-191 in HepG2 cells
2
Quantifying miR-497 and CDKN2B-AS1/CDK6 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Trizol reagent (Life Technologies Corporation, Carlsbad, CA, USA) was employed to extract total RNA from tissues and cultured cells following the manufacturer’s instructions. The miR-497 expression level was detected by using the Taqman MicroRNA Reverse Transcription Kit and the miRNA qPCR Assay Kit (Applied Biosystems, Foster City, CA, USA). For detection of CDKN2B-AS1 or cyclin-dependent kinase 6 (CDK6) mRNA expression, complementary DNA was synthesized from 2 μg of total RNA using cDNA Synthesis SuperMix kit (TaKaRa, Dalian, China) and then was quantified by using SYBR-Green Real-Time Master Mix (TaKaRa). All reactions were conducted using 7500 Real Time PCR System (Applied Biosystems, Foster City, CA, USA). The expressions of CDKN2B-AS1/CDK6 and miR-497 were calculated using 2−ΔΔCt method and normalized to GAPDH and U6, respectively.
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