Log In Sign up
The largest database of trusted experimental protocols

Anti cd4 macs beads

Manufactured by BD
Visit Supplier

Anti-CD4 MACS beads are magnetic microbeads coated with antibodies that specifically bind to the CD4 antigen on the surface of cells. These beads can be used to isolate and enrich CD4-positive cells from complex cell mixtures.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd4 and anti cd8 macs beads, by Miltenyi Biotec (2 mentions) Facsaria, by BD (2 mentions)

Close spelling variants of this product

Anti-CD4

2 protocols using anti cd4 macs beads

1

Purification and Transfer of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD45.1+ CD4+ and CD45.1+ CD8+ T cells were purified from the spleens of naïve B6.CD45.1+ mice by positive selection with anti-CD4 and anti-CD8 MACS beads (Miltenyi Biotec). CD4+Foxp3+CD25hi, CD4+Foxp3+CD25lo and CD4+Foxp3− T cells were sorted from spleens of Foxp3-DTR/GFP mice using a FACSAria (BD Biosciences) after positive selection with anti-CD4 MACS beads. Sorted cells (5 × 105) were transferred intravenously into naïve Tcrb−/−Tcrd−/− recipient mice. All sorted T-cell subsets were more than 95% pure.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
+ Open protocol
+ Expand
2

Purification and Transfer of T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD45.1+ CD4+ and CD45.1+ CD8+ T cells were purified from the spleens of naïve B6.CD45.1+ mice by positive selection with anti-CD4 and anti-CD8 MACS beads (Miltenyi Biotec). CD4+Foxp3+CD25hi, CD4+Foxp3+CD25lo and CD4+Foxp3− T cells were sorted from spleens of Foxp3-DTR/GFP mice using a FACSAria (BD Biosciences) after positive selection with anti-CD4 MACS beads. Sorted cells (5 × 105) were transferred intravenously into naïve Tcrb−/−Tcrd−/− recipient mice. All sorted T-cell subsets were more than 95% pure.
Botta D., Fuller M.J., Marquez-Lago T.T., Bachus H., Bradley J.E., Weinmann A.S., Zajac A.J., Randall T.D., Lund F.E., León B, & Ballesteros-Tato A. (2017). Dynamic regulation of T Follicular Regulatory cell responses by interleukin 2 during influenza infection. Nature immunology, 18(11), 1249-1260.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!