Anti flag m2 or rabbit

Manufactured by Merck Group

Anti-Flag (M2 or rabbit) is a laboratory reagent used in research applications. It is designed to detect and bind to specific protein tags, enabling the identification and analysis of tagged target proteins. The core function of Anti-Flag is to serve as a detection tool for researchers studying protein expression, localization, and interactions.

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Lab products found in correlation

Anti ar n 20, by Santa Cruz Biotechnology (1 mentions) Anti mdc1, by Abcam (1 mentions) Anti ar441, by Thermo Fisher Scientific (1 mentions) Anti gcn5, by Santa Cruz Biotechnology (1 mentions) Anti hp1 c1a9, by Developmental Studies Hybridoma Bank (1 mentions) Anti p21, by Thermo Fisher Scientific (1 mentions) Anti c myc, by Santa Cruz Biotechnology (1 mentions) Anti erα f10, by Santa Cruz Biotechnology (1 mentions)

2 protocols using anti flag m2 or rabbit

Antibody Panel for Epigenetic Study

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The antibodies used in this study were: anti-Flag (M2 or rabbit, Sigma), anti-trimethyl H3-K9, anti-AcH3, anti-AcH4, anti-trimethyl H3-K27, anti-AcH4-K16, anti-AcH3-K9 (Upstate Biotechnology), anti-HP1 C1A9 (Developmental Studies Hybridoma Bank at the University of Iowa), anti-GCN5, anti-AR (N-20) (Santa Cruz Biotechnology), anti-MDC1 (Abcam), anti-MYST1 (Bethyl laboratories), anti-AR 441, anti-p21WAF1 Ab-11 (Thermo scientific), anti-GAPDH (Shanghai Kangchen).

Wang C., Sun H., Zou R., Zhou T., Wang S., Sun S., Tong C., Luo H., Li Y., Li Z., Wang E., Chen Y., Cao L., Li F, & Zhao Y. (2015). MDC1 functionally identified as an androgen receptor co-activator participates in suppression of prostate cancer. Nucleic Acids Research, 43(10), 4893-4908.

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Cloning and Characterization of MDC1 Variants

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Human MDC1 cDNA coding sequence was amplified by PCR using KIAA0170 plasmid from the HUGE database (generous gift from Dr. T. Nagase) as a template. The human full length wild-type MDC1 cDNA (MDC1wt) or a series of truncated mutants (MDC1 N1, MDC1 N2, MDC1 N3, MDC1 C) were cloned in a pcDNA3.1-Flag vector containing a sequence encoding a Flag epitope upstream of the cloning site, to generate Flag-MDC1, Flag-MDC1 N1~N3, Flag-MDC1 C. The identities of constructs were verified by sequencing. The expression plasmid for human ERα (pSG5-ERα) and pGL-ERE-AdML reporter plasmid carrying three consensus estrogen response elements (3×ERE) were from Dr. Shigeaki Kato 33 (link), 34 (link).
The antibodies used in this study were: anti-MDC1 (Bethyl laboratories), anti-ERα (F10, Santa Cruz Biotechnology), anti-Flag (M2 or rabbit, Sigma), anti-c-Myc (Santa Cruz Biotechnology), anti-pS2 (Shanghai Sangon Biotech), anti-p21 (thermo), anti-GAPDH (Shanghai Kangchen).

Zou R., Zhong X., Wang C., Sun H., Wang S., Lin L., Sun S., Tong C., Luo H., Gao P., Li Y., Zhou T., Li D., Cao L, & Zhao Y. (2015). MDC1 Enhances Estrogen Receptor-mediated Transactivation and Contributes to Breast Cancer Suppression. International Journal of Biological Sciences, 11(9), 992-1005.

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