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Gt rosa 26sor loxp stop loxp rfp

Manufactured by Jackson ImmunoResearch

The Gt(ROSA)26Sor-loxp-STOP-loxp-RFP is a reporter mouse line that contains a Cre-inducible RFP (red fluorescent protein) allele at the Rosa26 locus. The expression of RFP is dependent on Cre-mediated recombination, allowing for the visualization of Cre-expressing cells.

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2 protocols using gt rosa 26sor loxp stop loxp rfp

1

Generation and Characterization of Genetically Modified Mice

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Ccr10-knockout/Egfp-knockin mice on C57BL/6 background (CD45.2+) were described24 (link), and crossed to B6 mice bearing CD45.1 alleles (Jackson lab) to obtain Ccr10EGFP/EGFP and Ccr10+/EGFP mice bearing CD45.1 and/or CD45.2 alleles. Rag1−/− mice, Rorc−/− mice, Ccr6−/− mice, Foxp3−/−, Foxn1−/−, Foxn1Cre/Cre, Gt(ROSA)26Sor-loxp-STOP-loxp-RFP (Gt(ROSA)26SorRFP/RFP), MuLanDTR and Foxp3-RFP reporter mice were all purchased from Jackson lab. HuLanDTR mice were previously described36 (link). Ahr−/− mice were kindly provided by Christopher Bradfield (University of Wisconsin). All the mice were on C57BL/6 background (CD45.2+). These mice were also crossed to Ccr10-knockout/Egfp-knockin mice on proper CD45.2+ and CD45.1+ C57BL/6 background to introduce one or two Ccr10-knockout/Egfp-knockin alleles for the purpose of reporting the CCR10 expression with EGFP or total Ccr10-knockout. Il2rg−/−Rag2−/− mice were from Taconic. All mouse experiments were performed in specific pathogen-free conditions (SPF) in accordance with protocols approved by The Pennsylvania State University Institutional Animal Care and Use Committee.
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2

Generation and Characterization of Genetically Modified Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ccr10-knockout/Egfp-knockin mice on C57BL/6 background (CD45.2+) were described24 (link), and crossed to B6 mice bearing CD45.1 alleles (Jackson lab) to obtain Ccr10EGFP/EGFP and Ccr10+/EGFP mice bearing CD45.1 and/or CD45.2 alleles. Rag1−/− mice, Rorc−/− mice, Ccr6−/− mice, Foxp3−/−, Foxn1−/−, Foxn1Cre/Cre, Gt(ROSA)26Sor-loxp-STOP-loxp-RFP (Gt(ROSA)26SorRFP/RFP), MuLanDTR and Foxp3-RFP reporter mice were all purchased from Jackson lab. HuLanDTR mice were previously described36 (link). Ahr−/− mice were kindly provided by Christopher Bradfield (University of Wisconsin). All the mice were on C57BL/6 background (CD45.2+). These mice were also crossed to Ccr10-knockout/Egfp-knockin mice on proper CD45.2+ and CD45.1+ C57BL/6 background to introduce one or two Ccr10-knockout/Egfp-knockin alleles for the purpose of reporting the CCR10 expression with EGFP or total Ccr10-knockout. Il2rg−/−Rag2−/− mice were from Taconic. All mouse experiments were performed in specific pathogen-free conditions (SPF) in accordance with protocols approved by The Pennsylvania State University Institutional Animal Care and Use Committee.
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