The largest database of trusted experimental protocols

16 protocols using veroclear

1

Fabrication of Multi-Material Microfluidic Valves

Check if the same lab product or an alternative is used in the 5 most similar protocols
The PFG and the NFG consisted of several layers of different 3D‐printed materials, as shown in Figure S1, Supporting Information. A multi‐material printer (Connex500, Stratasys, Ltd.) was used to print the top, hole, and bottom layers with a rigid plastic (VeroClear, Stratasys, Ltd.), and the poppet and O‐ring with a rubber‐like material (TangBlackPlus/Shore A30, Stratasys, Ltd.). A multi‐material printer (Objet500 Connex3, Stratasys, Ltd.) was used to print the membrane layer with a composite material DM 9840/Shore A40 (primary material: VeroClear, secondary material: Agilus/Shore A30, Stratasys, Ltd.) for improved mechanical properties. Furthermore, a 400‐µm thick membrane core was printed with DM 9870/Shore A70 (primary material: VeroClear, secondary material: Agilus/Shore A30, Stratasys, Ltd.) inside the 800‐µm thick diaphragm membrane as a reinforcement. All layers were assembled using a commercial instant adhesive (LOCTITE 4850, Henkel AG & Company, KGaA), including silicone tubing (1.6 mm ID and 3.2 mm OD, McMaster‐Carr) on each terminal. A surface primer (LOCTITE SF 770, Henkel AG & Company, KGaA) was used to promote adhesion between the silicon tubing and 3D‐printed parts. Finally, male and female of plastic quick‐turn tube couplings (for 1.6 mm barbed tube ID, McMaster‐Carr) were used to connect the fluidic gates.
+ Open protocol
+ Expand
2

Reinforced Hydrogel Composite Mechanical Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols
All the uniaxial tensile/compressive tests were conducted on an MTS uniaxial testing machine (Criterion model 43, MN, USA). For the comparison of the stretch-stress behavior between the AP hydrogels initiated by TPO and APS-TEMED (Fig. 3C), we prepared the hydrogel samples consisting of 80 wt % water and 20% AP mixture with the PEGDA (700)/acrylamide mixing ratio of 1.25 wt %. For the bonding demonstrations and tests (Fig. 3, D to Q), we prepared the hydrogel consisting of 80 wt % water and 20% AP mixture with the PEGDA (700)/acrylamide mixing ratio of 0.625 wt %. For the uniaxial tensile test of the horseshoe structure–reinforced hydrogel composite (Fig. 4B), we printed the matrix with the hydrogel consisting of 80 wt % water and 20% AP mixture with the PEGDA (700)/acrylamide mixing ratio of 0.625 wt % and printed the horseshoe structure with VeroBlack from Stratasys Ltd. (Eden Prairie, MN, USA). For the uniaxial compressive tests for the lattice-reinforced hydrogel cubes (Fig. 4, D to F), we printed the matrix with the hydrogel with the same recipe as the one used above and printed the lattice structures with VeroClear from Stratasys Ltd. (Eden Prairie, MN, USA) added with 0.05% Sudan I (Sigma-Aldrich) to improve the printing resolution. The same solutions were used to print lattice-reinforced meniscus (Fig. 4, G to K).
+ Open protocol
+ Expand
3

3D Printed Biomedical Device Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols
Veroclear and SUP706, used for 3D printing, were purchased from Stratasys (Rehovot, Israel). The medical epoxy (Epo‐Tek 301) was obtained from Epoxy Technology (MA, USA). The magnets were obtained from Daehan Magnet (Seoul, Korea). Titanium membranes with a thickness of 2 μm were purchased from Nilaco (Tokyo, Japan). Acetonitrile (ACN) was obtained from Daejung (Siheung, Korea). Phosphate buffered saline (PBS) and formalin were purchased from Thermo Fisher Scientific (MA, USA). Epinephrine, trifluoroacetic acid (TFA), and acetaminophen were purchased from Sigma‐Aldrich (MO, USA). Glucagon was obtained from Novo Nordisk (Bagsværd, Denmark). The adrenaline/Epinephrine enzyme‐linked immunoassay (ELISA) kit was purchased from MyBioSource (CA, USA). The rat Glucagon enzyme immunoassay (EIA) kit was obtained from RayBiotech (GA, USA). Gentamicin was purchased from Shinpoong (Seoul, South Korea).
+ Open protocol
+ Expand
4

Fabrication of Stretchable PDMS Chambers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were cultured in PDMS stretch chambers until they were 80% confluent. To create the stretch chambers, a mold was designed with SolidWorks and was fabricated using an Objet500 Connex3 PolyJet 3D Printer with VeroClear (Stratasys). The molds were baked in an oven for 2 h at 80 °C to allow them to outgas, which allows PDMS to fully crosslink. PDMS (Dow) was made by mixing the base and crosslinker at a 1:10 ratio and desiccating to remove bubbles. The PDMS was then cast into the mold and placed into an oven for 2 h at 80 °C to facilitate crosslinking, after which the stretch chamber was removed from the mold. A 1 × 1 in piece of 0.005 in silicone (Specialty Manufacturing) was then bonded to the stretch chamber using an oxygen plasma treatment. The chamber and silicone sheet were placed in a plasma cleaner (Harrick Plasma) and ran using ambient air for 45 s, after which the two pieces were joined and baked in an oven at 80 °C for 1 h to promote crosslinking between the two parts.
+ Open protocol
+ Expand
5

Tunable Acoustic Metasurface Design

Check if the same lab product or an alternative is used in the 5 most similar protocols
The unit cells were fabricated by 3D polyjet printing (J750, Stratasys, US) with photopolymers (VeroClear, Stratasys, US) that have similar material properties as acrylics and much higher acoustic impedance than that of air. A metasurface was assembled with the fabricated unit cells that were distributed in a linear array with a period of 27.8 mm. The cavity sizes of shunted Helmholtz resonators in the metasurface were controlled by pumping water into/out of the resonators with automated multimodule syringe pumps (Cetoni, Nemesys, Germany).
+ Open protocol
+ Expand
6

Additive Manufacturing of Biomedical Devices

Check if the same lab product or an alternative is used in the 5 most similar protocols
Materials for the Stratasys J750 PolyJet printer (VeroClear, MED610, and Tango+) were obtained directly from Stratasys (Eden Prairie, MN). ATP, crude firefly lanterns, HBSS, tris (hydroxymethyl)-aminomethane, CaCl2, NaCl, Mg(SO4), glucose, and glycerol were obtained from Sigma-Aldrich (St. Louis, MO). Luciferin was obtained from GoldBiotechnology (St. Louis, MO). KCl was obtained from Fisher Scientific (Fair Lawn, NJ). Bovine serum albumin was obtained from MP Biomedical.
+ Open protocol
+ Expand
7

Customized Microfluidic Insulin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Veroclear and SUP706 were purchased from Stratasys (Rehovot) as 3D‐printing and supporting materials, respectively. The stainless‐steel springs were purchased from Tohatsu. Polyurethane films, 100 μm in thickness, and Teflon molds, were obtained from CY International and 3DMD, respectively. Rubber piston stoppers were obtained by disassembly from a 0.3 ml BD ultra‐fine insulin syringe. Medical epoxy (Epo‐tek 301) was purchased from Epoxy Technology. Phosphate‐buffered saline (PBS) and formalin were obtained from Thermo Fisher Scientific. The check valves were purchased from Minivalve. Trifluoracetic acid (TFA) was purchased from Sigma‐Aldrich. Exenatide (MW = 4187 Da) was purchased from Cosmogenetech. Both insulin and glucagon were obtained from NovoNordisk.
+ Open protocol
+ Expand
8

3D Printing with Customized Supports

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isopropyl alcohol was obtained from Thermo-Fisher Scientific (St Louis, MO). Glycerol, sodium dodecyl sulfate (SDS), acridine orange and Tween20 were purchased from Sigma Aldrich (St. Louis, MO). VeroClear and Agilus30 were purchased from Stratasys (Eden Prairie, MN). Liquid support for the 3D printer was either pure Tween20 or a 60:40 (by volume) mixture of Glyrcol:IPA.
+ Open protocol
+ Expand
9

Characterization of VeroClear-RGD 810 Resin

Check if the same lab product or an alternative is used in the 5 most similar protocols
The printable material of interest is a type of resinous material with the trade name VeroClear-RGD 810, which is a rigid, nearly colourless material exhibiting dimensional stability. It is an epoxy-based polymer with a polymerized density of 1.18–1.19 g/cm3 at room temperature. Additionally, it has a water absorption of 1.1–1.5%, Rockwell hardness of 73–76 scale M, and a heat distortion temperature (HDT) of approximately 45–50 °C. VeroClear was supplied by Stratasys Ltd., Israel. It is available for Objet Connex 3D printers.
+ Open protocol
+ Expand
10

Fibrin Gel Preparation for Tensile Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Homogeneous fibrin gels were prepared to final fibrinogen concentrations of 2 and 4mg/mL by combining human fibrinogen (FIB3, Enzyme Research Laboratories) and Alexa Fluor (AF) 488 or 546 conjugated human fibrinogen (F13191, Molecular Probes) at a 1:10 fluorescent to non-fluorescent fibrinogen ratio, 1μL 2M CaCl2 , and 0.5U thrombin (HT 1002A, Enzyme Research Laboratories) per mg fibrinogen in phosphate buffered saline (PBS). Immediately after mixing, the solution was pipetted into molds containing frames that enabled tensile testing (Fig. 1ac) and allowed to polymerize for 5 minutes. Molds (2.5×6.6×0.75 mm) were designed in SolidWorks and 3D printed (Stratasys J750) with a photopolymer (VeroClear, Stratasys) that is similar to acrylic. Frames (6×12mm) were drawn in Adobe Illustrator and laser cut from 100μm thick polyethylene terephthalate using a Speedy 360 laser cutter (Trotec). Polyethylene (PE) blocks (coarse grade, Bel-Art Products) were manually cut to approximately 1.5mm×2.0mm×1.5mm . To increase wettability of PE by the fibrinogen solution, the blocks were submerged in 70% ethanol for 5 minutes at room temperature (RT), submerged in 12.91mg/mL fibrinogen solution for at least 1 hour, then glued to the laser-cut frames with cyanoacrylate adhesive.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!