Chloroquine diphosphate

Manufactured by Selleck Chemicals

Sourced in United States

Chloroquine diphosphate is a chemical compound commonly used in laboratory settings. It is a white, crystalline powder that is soluble in water. The primary function of chloroquine diphosphate is as a reagent for various chemical and biological research applications.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using chloroquine diphosphate

KSHV Latency Model for Anti-COVID-19 Drugs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human iSLK.219 cells are latently infected with a recombinant rKSHV.219 virus and contain a doxycycline (Dox)-inducible RTA, constructed and named by Dr. Don Ganem’s lab^{12 (link)}. The rKSHV.219 virus expresses both the red fluorescent protein (RFP) under the control of KSHV lytic PAN promoter and the green fluorescent protein (GFP) under the control of the elongation factor 1 promoter (EF-1α)^{30 (link)}. HEK293T (Human embryonic kidney 293T) cells and KSHV + PEL cell lines, BCP-1 and BCBL-1, were purchased from American Type Culture Collection (ATCC) and cultured as recommended by the manufacturer. The anti-COVID-19 drugs: Azithromycin, Chloroquine diphosphate, Hydroxychloroquine sulfate, Nafamostat mesylate, Remdesivir, and Tocilizumab were purchased from Selleck Chemicals. All the other chemicals if not indicated specifically were purchased from Sigma-Aldrich.

Chen J., Dai L., Barrett L., James J., Plaisance-Bonstaff K., Post S.R, & Qin Z. (2021). SARS-CoV-2 proteins and anti-COVID-19 drugs induce lytic reactivation of an oncogenic virus. Communications Biology, 4, 682.

+ Open protocol

+ Expand

Characterization of Glial Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human normal glial cell and glioma cell lines were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cell lines were characterized by DNA fingerprinting, cell vitality detection, isozyme detection, and mycoplasma detection. The last cell characterization was performed in December 2020. The five cell lines were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Bethesda, MD, USA) and pen/strep antibiotics. All cells were incubated in 5% CO₂ at 37°C. Cycloheximide, proteasome inhibitor MG132, and chloroquine diphosphate were purchased from Selleck Chemicals (Houston, TX, USA).

Yang H., Liu X., Zhu X., Zhang M., Wang Y., Ma M, & Lv K. (2022). GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. iScience, 25(9), 104952.

+ Open protocol

+ Expand

Epigenetic and Metabolic Compound Library Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detailed lists of all 128 compounds included in the epigenetics compound library (L1900, Selleckchem, Houston, TX, USA) and of all twenty compounds included in the custom designed compound library for the HDAC inhibitor combination drug screen are available in the Supplementary Files (Table S3 and Table S4, respectively). The IDH1 mutant (R132H and R132C) inhibitor AGI-5198 (14624, Cayman Chemical, Ann Arbor, MI, USA), the cell permeable derivative of D-2-HG ((2R)-Octyl-α-hydroxyglutarate, 16366, Cayman Chemical), the HDAC inhibitor romidepsin (S3020, Selleckchem), the glutamate dehydrogenase inhibitor chloroquine diphosphate (S4157, Selleckchem), the glutaminase inhibitor metformin HCl (S1950, Selleckchem) and the B-cell lymphoma 2 (Bcl-2) family member inhibitors ABT-737 (Bcl-2/Bcl-xL/Bcl-w, S1002, Selleckchem), S63845 (Mcl-1, S8383, Selleckchem), venetoclax (Bcl-2, S8048, Selleckchem), and WEHI-539 (Bcl-xL, A3935, APExBIO, Houston, TX, USA) were dissolved in DMSO, PBS, or RPMI 1640 medium according to the manufacturer’s protocol.

Venneker S., Kruisselbrink A.B., Baranski Z., Palubeckaite I., Briaire-de Bruijn I.H., Oosting J., French P.J., Danen E.H, & Bovée J.V. (2020). Beyond the Influence of IDH Mutations: Exploring Epigenetic Vulnerabilities in Chondrosarcoma. Cancers, 12(12), 3589.

+ Open protocol

+ Expand

Antibody Procurement for EV71 Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse anti-Flag (F1804) and rabbit anti-HA (H6908) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Rabbit anti-GFP (50430-2-AP), Rabbit anti-LC3 (14600-1-AP) and Rabbit anti-P62 Polyclonal antibody(18420-1-AP) were purchased from Proteintech Group (Chicago, IL, USA). Rabbit anti-β-actin (AC026) and anti-EV71-3D polyclonal antibodies were produced by ABclonal Technology (Wuhan, China). Rabbit anti-EV71-VP1 (PAB7631-D01P) polyclonal antibody was purchased from Abnova (Taipei city, Taiwan). Rabbit anti-Beclin1 (381896) antibody was purchased from ZENBIO (Chengdu, China). Delbecco modified Eagle medium (DMEM), and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Lipofectamine 2000, normal rabbit IgG and normal mouse IgG were purchased from Invitrogen Corporation (Carlsbad, CA, USA). Protein ladder (26616) was purchased from Thermo scientific (Rockford, IL, USA). Complete, EDTA-free Protease Inhibitor Cocktail was purchased from Roche (Basel, Switzerland). Chloroquine diphosphate were purchased from Selleck (Houston, TX, USA). Rapamycin (53123-88-9) was obtained from Target Mol (Shanghai, China).

Xiang Q., Wan P., Yang G., Huang S., Qin M., Yang H., Luo Z., Wu K, & Wu J. (2020). Beclin1 Binds to Enterovirus 71 3D Protein to Promote the Virus Replication. Viruses, 12(7), 756.

+ Open protocol

+ Expand

Gastric Cancer Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GC cell lines SGC7901, BGC823, AGS, MKN45, and SNU-719, and the gastric mucosal epithelial cell line GES1 were purchased from the Cobioer Biosciences Co., Ltd (Nanjing, China). All cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Gibco, Grand Island, NY, USA). Cycloheximide, proteasome inhibitor MG132, and chloroquine diphosphate were purchased from Selleck Chemicals (Houston, TX, USA).

Zhang C., Ma X., Wei G., Zhu X., Hu P., Chen X., Wang D., Li Y., Ruan T., Zhang W., Tao K, & Wu C. (2022). Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience, 26(1), 105745.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!