Anti psma6

Expression of viral and host cellular proteins was detected by Western blotting according to the procedure described previously (10 (link)). Equal amounts (10 to 30 μg) of protein samples were separated using 10 to 12% SDS-PAGE gels and transferred to 0.2-μm nitrocellulose membranes. Anti-PSMA1 (Invitrogen; catalog no. PA1-963), anti-PSMA2 (Cell Signaling; catalog no. 2455), anti-PSMA6 (Invitrogen; catalog no. PA576058), anti-beta-actin (Cell Signaling; catalog no. 3700S), anti-STAT3 (Cell Signaling; catalog no. 9139S), anti-CST3 (Cell Signaling; catalog no. 4280), and in-house-prepared IAV mouse-anti-NS1 and mouse-anti-NP (93 (link)) were used to detect specific proteins. Appropriate secondary horseradish peroxidase (HRP)-conjugated horse anti-mouse or anti-rabbit (Cell Signaling; catalog no. 7076 and 7074, respectively) were used to detect immune complexes. Protein bands were developed with ECL reagents and captured by Alpha Innotech FluorChemQ MultiImage III. The differences in protein expression were determined by measuring band intensities with ImageJ 1.50i (NIH, USA). The data were analyzed and graphically presented by GraphPad Prism v 9.1.0 software.

The anti‐microtubule‐associated protein antibodies 1A/1B light chain 3B (LC3B), anti‐Erk 1/2, anti‐STAT5, anti‐Akt, anti‐ubiquitin (P4D1) and anti‐PSMB5 were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti‐FLT3, anti‐p62/SQSTM1 and anti‐Beta 2 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and anti‐PSMA6 was obtained from Invitrogen (Carlsbad, CA, USA). Anti‐PSMB6 and anti‐Rpn10 were purchased from Enzo (Enzo Diagnostics, Farmingdale, NY, USA), anti‐PSMD3 was obtained from Thermo Fisher (Waltham, MA, USA) and anti‐GAPDH was obtained from Sigma‐Aldrich (St Louis, MO, USA).
Bz and chloroquine were purchased from Sigma‐Aldrich, BafA was obtained from InvivoGen (InvivoGen, San Diego, CA, USA) and VT was obtained from Sigma‐Aldrich.