Primary normal human astrocytes (NHAs) were obtained from ScienCell Research Laboratories. Glioma cells including U373MG, LN-Z308, LN319, LN444, LNN382T, SNB19, U87MG, and LN464 were purchased from ATCC or Cell Bank of Chinese Academy of Sciences and maintained in high-glucose DMEM (Hyclone, Logan, UT, USA) supplemented with 10% FBS (Excell, Shanghai, China).
Primary normal human astrocytes nhas
Manufactured by ScienCell
Sourced in United States
Primary normal human astrocytes (NHAs) are a type of glial cell that originate from the human central nervous system. They provide essential support and functions for neurons.
Automatically generated - may contain errors
Lab products found in correlation
U87mg, by American Type Culture Collection (2 mentions)
U118mg, by American Type Culture Collection (2 mentions)
U138mg, by American Type Culture Collection (2 mentions)
U373mg, by American Type Culture Collection (1 mentions)
Lnz308, by American Type Culture Collection (1 mentions)
Ln444, by American Type Culture Collection (1 mentions)
Snb19, by American Type Culture Collection (1 mentions)
Tj905, by American Type Culture Collection (1 mentions)
Ln229, by American Type Culture Collection (1 mentions)
Sw1088, by American Type Culture Collection (1 mentions)
Hcmec d3, by ScienCell (1 mentions)
Hek293t, by Cell Resource Center (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mycaway plus color one step mycoplasma detection kit, by Yeasen (1 mentions)
6 protocols using primary normal human astrocytes nhas
1
Primary Astrocytes and Glioma Cell Culture
2
Cell Culture Protocol for Glioblastoma
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Primary normal human astrocytes (NHAs) were purchased from Sciencell Research Laboratories (Carlsbad, CA, USA). Human U87, A172, U251, TJ905, and U373 glioblastoma cell lines were purchased from the ATCC (Manassas, VA, USA). NHAs were cultured in Dulbecco’s modified Eagle’s medium (DMEM), but all glioma cell lines were cultured in DMEM-F12; both mediums were supplemented with 10% fetal bovine serum (FBS). The mediums and FBS were purchased from Invitrogen (Carlsbad, CA). All cells were maintained in a 37°C, 5% CO2 incubator.
3
Establishing Primary Astrocyte and Glioma Cell Cultures
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Primary normal human astrocytes (NHAs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured according to the manufacturer’s instructions. Glioma cell lines A172, T98G, LN18, LN229, U138MG, U87, and U118MG were from ATCC (Manassas, VA, USA). The cells were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.
4
Isolation and Culture of Brain Tumor Cells
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Primary normal human astrocytes (NHAs) were purchased from Sciencell Research Laboratories. The glioma cell lines U-118MG, U-87MG, A-172, SW 1088, SW 1788 and LN-18 were purchased from American Type Culture Collection (ATCC). These cells were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS). U138MG was also purchased from ATCC and cultured in DMEM supplemented with 10% FBS. Fresh brain tumor tissues that were clinically histopathologically diagnosed at the Sun Yat-sen University-Affiliated First Hospital were used. Patient’s consent and approval from the Institutional Research Ethics Committee were acquired for use of data for the research. All the tissues were collected and processed within 30 min after resection. The primary cultured tumor cells were obtained after mechanical dissociation, as previously described [39 (link)].
5
Culturing Primary Human Glioma and EC Cells
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Human glioblastoma cell line U87 and human embryonic kidney 293T (HEK293T) cells were purchased from Shanghai Institutes for Biological Sciences Cell Resource Center. Primary normal human astrocytes (NHAs) were purchased from the ScienCell Research Laboratories (Carlsbad, CA, USA), and the immortalized human cerebral microvascular EC line hCMEC/D3 was acquired from Dr. Couraud (Institute Cochin, Paris, France). Glioma conditioned medium was obtained from the U87 glioblastoma cells, and the ECs and astrocytes conditioned medium were obtained from the NHA cells and the ECs. We cultured U87 cells, HEK293T cells, NHA cells, and ECs and obtained glioma conditioned medium and astrocytes conditioned medium as previously described.45 (link) For details, see Supplemental Materials and Methods . Then we got the GECs for the subsequent experiments.
6
Glioblastoma Cell Line Cultivation
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Human U87, U251, A172, and U373 glioblastoma cell lines were purchased from the ATCC (Manassas, VA, USA). Primary normal human astrocytes (NHAs) were from Sciencell Research Laboratories (Carlsbad, CA, USA). NHAs were cultured in Dulbecco’s modified Eagle’s medium (DMEM), while all cultured glioma cell lines were cultured in DMEM-F12. Both mediums were supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and incubated at 37°C and 5% CO2. No mycoplasma contamination was detected in all cell lines by the MycAwayTM Plus-Color One-Step Mycoplasma Detection Kit (Yeasen, Shanghai, China).
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