Lc msd trap xct plus mass spectrometer

The HPLC-MSⁿ analysis was performed with an Agilent 1100 LC system equipped with an LC/MSD Trap XCT Plus mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). A TC-C18 (4.6 mm × 250 mm, 5 μm) column (Agilent) was used for analysis. The column temperature was kept at 30 °C. The mobile phase consisted of acetonitrile (A) and water containing 0.2% phosphoric acid (B). The following gradient condition was used: 0–10 min, 5–15% A; 10–30 min, 15–30% A; 30–42 min, 30–42% A; 42–45 min, 42–48% A; 45–50 min, 48–60% A; and 50–60 min, 60–90% A. The mobile phase flow rate was 1 mL/min and the sample injection volume was 10 μL. The detected wavelength was 238 nm. Mass spectra were acquired in positive and ion modes with an ESI source in the range of m/z 100 to 1000. The ESI-MS conditions were that the nebulizer pressure at 45 psi and nitrogen as the drying gas at a flow rate of 10 L/min with a temperature of 350 °C. The capillary voltage was set at 3500 V. Data were acquired by use of the Agilent Chemstation software (Agilent Technologies).

The LC-ESI-MSⁿ analysis was performed with an Agilent 1100 LC system with an LC/MSD Trap XCT Plus mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). A TC-C18 (4.6 mm × 250 mm, 5 μm, Agilent) column was used for analysis. The column temperature was kept at 30 °C. The mobile phases consisted of methanol (A) and water containing 1% formic acid (B). The following gradient condition was used: 0–10 min, 10% A; 10–12 min, 10%–30% A; 12–30 min, 30%–35% A; 30–35 min, 35%–40% A; 35–40 min, 40%–55% A; 40–50 min, 55%–66% A; and 50–60 min, 66%–100% A. The mobile phase flow rate was 0.8 mL/min and the sample injection volume was 10 μL. The detected wavelength was 254 nm. Mass spectra were acquired in both positive and negative ion modes with an ESI source in the range of m/z 100 to 1000. The ESI-MS conditions were: the nebulizer pressure at 45 psi and nitrogen as the drying gas at a flow rate of 10 L/min with a temperature of 350 °C. The capillary voltage was set at 3500 V. Data were acquired by use of Agilent Chemstation software (Agilent Technologies).