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Objet500 connex3

Manufactured by Stratasys
Sourced in United States

The Objet500 Connex3 is a multi-material 3D printer designed for professional use. The core function of this equipment is to enable the creation of complex, multi-material 3D printed parts and prototypes.

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17 protocols using objet500 connex3

1

Leaf-Inspired 3D-Printed Materials: Mechanical Testing

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Leaf-inspired materials for mechanical testing were 3D-printed using a Stratasys Connex3 Objet500 printer in the Stratasys digital material FLX-MK-S50-DM, which has a Shore A hardness value of 50. Other samples were printed using an Ultimaker S3 with TPU (thermoplastic polyurethane) and PLA (polylactic acid) filament (Fig. 2 and Supplementary Fig. 2) and an Anycubic Photon Mono X resin printer (Fig. 8).
Mechanical testing was performed using a 5 kN capacity Instron Universal Testing System. Samples were loaded under compression at 30 mm/min.
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2

Additive Manufacturing of 3D Tumor Phantoms

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The CAD models were translated to the printer in a printable Standard Tessellation Language (STL) format. First, the STLs of the 11 cubic samples (C00–C100) were transferred to the PolyJetTM printer Connex3 Objet500 (Stratasys Ltd., EdenPrairie, MN, USA). Then, 11-cubes in sets of two were printed, each set with a different material for the grid structure: solid bio-compatible MED610 (Stratasys, EdenPrairie, MN, USA) with a Shore hardness of 83-86 (Scale D) and flexible TangoPlus (Stratasys Ltd., EdenPrairie, MN, USA) with rubber-like properties with a Shore hardness of 26-28 (Scale A). SUP706 (Stratasys Ltd., Eden Prairie, MN) was chosen as the support material. The samples were printed with different matrix thicknesses as designed in the CAD software, yielding a specific partial volume contribution C% of the MRI-signal generating material to the voxel.
The spherical tumour models were printed using a different material for the grid structure, TangoPlus, VeroClear and VeroMagenta (Stratasys Ltd.). The general properties of VeroClear and VeroMagenta were similar to those of MED610 but not approved biocompatible. The same support material as for the cubic samples, SUP706, was used.
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3

3D Printed Prototyping of PMD Device

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Design of the PMD was performed in SolidworksTM. Prototypes were 3D printed using a Stratasys Connex 3 Objet500 with VeroClear material and silicone elastomer for the seal.
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4

Multi-Material 3D Printing of Skeletal Phantom

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The printable file in STL format was transported to a multi material PolyJetTM printer Connex3 Objet500 (Stratasys, Eden Prairie, MN, United States of America) and a 1:1 scale model of skeletal integument was additively manufactured. The available 3D printed materials: rigid Vero pure white (RGD837) for the sternum, ribs and vertebral column; flexible Agilus30 Clear (FLX935) for embedding the skeletal integument and SUP706B as the support material were used to build the phantom. The printer settings were multi material mode with a standard layer thickness of 30 micrometer. The printing process of both print jobs took 120 h and needed 9,6 kg of flexible, 3,8 kg hard and 9,6 kg of support material, respectively. A rigorous cleaning procedure followed post printing, starting with manual removal of the support material from the hollow skeletal integument, alternating with waterjet (KK 30-VA, Krumm-Tec, Germany). To get the best results the model was then placed into a 2% NaOH solution, which gradually dissolved the support material, followed by a wash up with water. The above mentioned cleaning steps were repeated over a week to assure total removal of the support material from the alveolar skeletal integument making room for inserting heterogenous materials.
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5

3D Printing of Congenital Diaphragmatic Hernia

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A segmentation model of the diaphragm and surrounding structures of a 37 + 5‐week CDH fetus was chosen for 3D model reconstruction with additive manufacturing, known colloquially as ‘3D printing’. The segmentation model was obtained, stored in nifti‐file format and converted to STL format for 3D printing. A Stratasys Objet 500 Connex 3 (Stratasys, Eden Prairie, MN, USA) 3D polymer printer was used to produce the model in polyjet technology. Four different rigid materials were assigned to the 3D model: VeroYellow (RGD836, 99g) for the intact diaphragm (yellow); VeroMagenta (RGD851, 11g) for the diaphragmatic defect (magenta); VeroClear (RGD810, 291g) for the liver (transparent); and VeroBlue (RGD840, 20g) for the inferior vena cava and hepatic veins (blue) (Figure 8).
A routine fetal MRI protocol at our institution takes approximately 45 min, of which 5–10 min are dedicated to fetal thoracic imaging. The image export and preparation, segmentation and reformatting required between 45 min and 1 h, depending on gestational age. 3D printing took 8.5 h, with an additional 1.5 h needed for removing the support material from the 3D model. The total time and cost required to produce the 3D printed CDH model were 12 h and 309.00, respectively (including working time and materials).
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6

3D Printed Patient-Specific Kidney Model

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From the DICOM data of patient’s MDCT, kidney structures of interest (tumor, healthy parenchyma, arterial tree, renal vein, collecting system) were extracted using an image recognition algorithm (Synapse 3D, Fujifilm, Tokyo, Japan) and transferred into STL format. Using this STL file, a 3D printed model was created on the OBJET 500 Connex 3 (Stratasys, Eden Prairie, MN, USA) with the assistance of a 3D printing manufacturing company (Fasotec, Makuhari, Chiba, Japan).
The color-segmented 3D model was manufactured by the combination of three different types of photopolymer materials (opaque magenta, opaque yellow and transparent clear material) with 16 μ thickness of each layer under solidification by UV.
As displayed in Figs. 1, 2 and 3, the arterial tree was represented in opaque magenta, the collecting system in opaque yellow and mixing magenta and yellow resulted in opaque orange for tumor display. The renal parenchyma and the renal vein were kept translucent to achieve the best visualization of the relationships between the tumor, the collecting system and the arterial branches.
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7

3D Printing Anatomical Aortic Valve Model

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In this work, the MM3DP approach developed by Hosny et al. (29 ) was optimized for hydrodynamic testing and used for comparison with our model. We used the algorithm developed in their work to generate the aortic valve leaflet geometries from landmarks of abdominal CT images with superimposed calcium-like nodules. However, instead of limiting the vascular anatomy to the aortic sinus, we integrated the valve leaflets and nodules into the entire LV and aortic (through the aortic arch segment) anatomies. First, this approach allowed us to conduct functional tests of their model of AS. Second, it enabled us to integrate their approach with our strategy of LV actuation, allowing for a fairer comparison between the two models of AS. The LV, aorta, aortic valve leaflets, and calcium nodules were printed simultaneously using an Objet 500-Connex3 3D printer (Stratasys) using the same printing techniques as described in the original publication (29 ). To do this, we created a small (2 to 5 mm in diameter) hole in proximity to the LV apex to remove any support material laid down during the manufacturing process. The hole was then sealed using the same resin material, and ultraviolet light was applied manually.
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8

3D Printing for Pediatric Visualization

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3D printing was performed by the Children’s Hospital Additive Manufacturing for Pediatrics (CHAMP) Lab, a central 3D printing lab administered by the Department of Radiology at CHOP. A material jetting printer, (Objet500 Connex 3 (prior to July 2019) and J750, Stratasys, Eden Prairie, MN), was used to create physical models at 100% scale. Prior to 2020, models were created using rigid photopolymers with a minimum thickness of 1.0 mm. After January 2020, the option to print models with a minimum thickness of 0.75 mm and using flexible rubber photopolymers was available (Fig. 2a, Supplemental Fig. 2).

3D Visualization Modalities. a) 3D printed model; b) Digital model viewed using a 3D PDF; c) Virtual Reality; d) CAD modeling of digital repairs. 3D = Three-dimensional; PDF = portable document file; CAD = computer aided design

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9

Kirigami Fabrication via Polyjet Printing

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Polyjet printing is a technology that is capable of fabricating complex multimaterial geometries by jetting and curing various photopolymer68 (link). We used two commercially available soft photopolymers (i.e., Agilus and TangoPlus) and a rigid photopolymer to fabricate viscoelastic kirigami using a commercial 3D printer (Objet500 Connex3, Stratasys, see Supplementary Information section A, B and C). To reach an effective strain rate and achieve high-speed mode of buckling, we developed a costume-made test bench with a low-friction Teflon substrate allowing us to adjust the speed of stretch in the range of 1 to 5000 mm/s. We then analyzed our experiments by tracking the embedded particles (i.e., black dots and monochrome patterns) on the surface of kirigams using Matlab codes and analyzed the snap-back of kirigami unit cells in 3D using a Python code (see Supplementary Information section D).
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10

3D-Printed Microfluidic Cartridge Fabrication

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3D computer-aided design (CAD) models of the cartridge were designed using Autodesk Inventor Professional 2019 software (Autodesk, Inc., San Rafael, CA, USA). The CAD data were exported in STL format for the 3D printer. A ProJet MJP 3600 MAX 3D printer (3D Systems, Rock Hill, SC, USA) in its maximum resolution mode (XHD mode; z-axis resolution of 16 μm) was used for cartridge printing using a translucent and biocompatible ultraviolet (UV)-curable acrylic resin (Visijet M3 Crystal; 3D Systems). The cartridge printing process took approximately 4 h in the XHD mode. The cartridge is composed of separate top and bottom parts. We also fabricated a transparent 3D-printed cartridge using the same CAD data for visualizing flow in the channels. Owing to material (RGD−810 VeroClear; Stratasys, Eden Prairie, MN, USA) and printer (Objet500 Connex3; Stratasys) limitations, transparent materials were printed with a 30 µm z-axis resolution.
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