Seven stage 4 primary CC tissues and four metastatic liver tissues were subjected to IHC staining. A FFPE tissue section slide was manually deparaffinized using m-Xylene (Sigma-Aldrich Corp, Saint Louis, MO, USA) and rehydrated using a series of 100% ethanol, 95% ethanol, and PBS washes. The prepared FFPE slide was dried, outlined with a hydrophobic barrier around the tissue, and then covered with a blocking solution (Dako, Agilent Technologies, CA, USA). The tissue was positioned in a humidity chamber and covered with a diluted primary antibody solution (1/100, anti-TSPAN8, ab230448, Abcam, Cambridge, UK) at 4 °C overnight. Subsequently, the tissue was treated with Dako HRP solution (30 min) and then Dako DAB solution (5 min) in a light-protected humidity chamber. Finally, the tissue was exposed to a solution of Dako hematoxylin (5 min), washed with water, mounted with ProLong Gold Antifade Mountant (Thermo Fisher Scientific, Waltham, MA, USA), and subjected to scanning using the Leica Asperio ScanScope CS system (Leica Camera AG, Wetzlar, Germany). ImageJ software (Version v.153, National Institutes of Health, Bethesda, MD, USA) was employed for quantifying IHC intensity in the images obtained. The p-value was calculated using the Mann–Whitney U-test.
M xylene
Manufactured by Merck Group
Sourced in United States, Germany, China
M-xylene is a colorless, flammable liquid chemical compound with the molecular formula C8H10. It is one of the three isomeric forms of xylene, which are widely used as solvents and in the production of other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
P xylene, by Merck Group (4 mentions)
O xylene, by Merck Group (3 mentions)
Ethylbenzene, by Merck Group (3 mentions)
Toluene, by Merck Group (3 mentions)
Methanol, by Merck Group (2 mentions)
Chlorobenzene, by Merck Group (2 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Avantor (1 mentions)
Sulfuric acid, by Avantor (1 mentions)
Nitric acid, by Avantor (1 mentions)
Calcium chloride, by Avantor (1 mentions)
Sodium bicarbonate, by Avantor (1 mentions)
Sodium sulfate, by Avantor (1 mentions)
Potassium phosphate tribasic, by Merck Group (1 mentions)
Calcium phosphate, by Merck Group (1 mentions)
Sodium phosphate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Dibutyl phthalate, by Merck Group (1 mentions)
2100f electron microscope, by JEOL (1 mentions)
Anhydrous butanol, by Merck Group (1 mentions)
21 protocols using m xylene
1
TSPAN8 Expression in Primary and Metastatic Tissues
2
Spectrophotometric Analysis of Inorganic Compounds
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All
materials were used
in their original form as received from commercial suppliers. Sodium
chloride (99.5–100%), sulfuric acid (37%), nitric acid (90%),
calcium chloride (95%), sodium bicarbonate (98%), and sodium sulfate
(≥98%) were purchased from VWR Chemicals. Sodium phosphate
(96%), m-xylene (98%), potassium phosphate tribasic
(97%), calcium phosphate (>96%), and magnesium phosphate (98%)
were
supplied by Sigma-Aldrich. DMSO (99%) used was purchased from Alfa
Aesar. All the salts used contained a common sodium cation in order
to eliminate any possible deviations induced by the use of different
cationic species. A Shimadzu UV-3600 UV–vis–NIR spectrophotometer
was used for the measurement and recording of UV spectra.
materials were used
in their original form as received from commercial suppliers. Sodium
chloride (99.5–100%), sulfuric acid (37%), nitric acid (90%),
calcium chloride (95%), sodium bicarbonate (98%), and sodium sulfate
(≥98%) were purchased from VWR Chemicals. Sodium phosphate
(96%), m-xylene (98%), potassium phosphate tribasic
(97%), calcium phosphate (>96%), and magnesium phosphate (98%)
were
supplied by Sigma-Aldrich. DMSO (99%) used was purchased from Alfa
Aesar. All the salts used contained a common sodium cation in order
to eliminate any possible deviations induced by the use of different
cationic species. A Shimadzu UV-3600 UV–vis–NIR spectrophotometer
was used for the measurement and recording of UV spectra.
3
Monitoring nitrogen utilization in P. putida
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Overnight cultures of P. putida mt‐2 in M9 medium (OD600nm ~0.8) were diluted 100 times in fresh medium and exposed to vapours of m‐xylene (Sigma‐Aldrich, St. Louis, MO, USA) stemming from a 1:5 dilution in dibutyl phthalate (Sigma‐Aldrich) in sealed flasks, basically as described in Velázquez et al. (2006 ). At OD600nm ~0.5, cells were washed twice in PBS before resuspending them in N‐free M9 medium supplemented with either 10 mM NO3− or NH4+ as nitrogen source and incubated in the presence of m‐xylene at 28°C with agitation at 150 r.p.m. The bottles were sealed and kept closed during the incubation. At 15 min, 30 min, 1, 3, 5 and 7 h after the shift in nitrogen sources, samples were withdrawn with a sterile syringe through a septum in the cap. Cells were pelleted by centrifugation at 4°C (10,000 g, 2 min), frozen in liquid N2 and immediately stored at −70°C until nucleic acid extraction.
4
Synthesis and Characterization of Colloidal Quantum Dots
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The CdSe/ZnS QDs for blue and green QDs and the CdSe/CdS/ZnS QDs for red QDs were synthesized in the laboratory. All QDs have CdSe–ZnS core-shell alloyed structures to enhance EL and show a PL quantum yield of >∼80%. The synthesis methods for the colloidal NCs are described in Supplementary Methods . Poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS; VP AI 4083) was purchased from Clevios, and TFB (poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4′-(N-(4-sec-butylphenyl))diphenylamine)], SOL 2036) was purchased from Solaris. Anhydrous butanol, heptane and m-xylene were purchased from Sigma-Aldrich. Zinc oxide NCs for the ETL, PbS QDs and CuInSe QDs were synthesized in the laboratory (details in Supplementary Methods ). Transmission electron microscopy images were obtained on a JEOL 2100F electron microscope. The absorption spectra were acquired on a CARY 5000E ultraviolet–visible–near-infrared spectrophotometer. PL and time-resolved fluorescence spectra were recorded on an FLS 980 spectrometer (Edinburgh Instruments). For PL, the QDs were excited with a steady-state xenon lamp, and the emitted photons were detected by a single-photon-counting photomultiplier. The valance band maximum of the layer materials was determined by the ultraviolet photoelectron spectroscopy (Thermo Fisher Scientific Co.) with a He discharge lamp (21.2 eV).
5
Synthesis of Inorganic Nanoparticles and Pollutant Remediation
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The TiO2, ZnO and RuO2 nanoparticles were synthesized using analytical grade titanium(iv ) butoxide (Ti(OCH2CH2CH2CH3)4), acetoxyzinc dihydrate (Zn(Ac)2·2H2O), and ruthenium trichloride trihydrate (RuCl3·3H2O) respectively, and these chemicals were obtained from Sigma Aldrich (Germany). To accomplish this study, the environmental toxins such as 1,4-dioxane, 3-chlorophenol, 2,4-dinitrophenol (2,4-DNP), chlorobenzene (CBZ), p-nitrophenol (p-NP), 4-nitrophenyl hydrazine (4-NPHyd), phenylhydrazine (PHyd), m-xylene, zimataldehyde, and pyridine were procured from Sigma-Aldrich and used as purchased. Besides this, Nafion (5% suspension in ethanol), and mono- and disodium phosphate were similarly acquired from Sigma Aldrich (Germany).
6
Synthesis of Benzothiadiazole Derivatives
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2,1,3-benzothiadiazole (>99.0%) and 1,4-dinitrobenzene (>99.0%) were purchased from TCI America. p-xylene, m-xylene, o-xylene, hexamethylphosphoramide and butyronitrile were purchased from Sigma Aldrich. Cyclohexanone was purchased from TCI America. Other solvents (>99.0% with extra dried condition) were purchased from Acros Organics and used without any pretreatment.
7
Determination of VOCs in Calypogeia azurea
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The following solvents were used during the research: n-hexane, puriss. p.a., ≥99% (GC), ethyl acetate, puriss. p.a., 99.9% (GC), methanol for GC, Sigma-Aldrich (Steinheim, Germany) and m-xylene, diethyl ether, puriss. ≥99.9% (GC), methylene chloride >99.9%, POCH (Gliwice, Poland). Saturated n-alkanes of C7-C40 standard Supelco (Bellefonte, PA, USA) were used to determine the Kovats retention indices.
Fused silica fibers coated with divinylbenzene/carboxy/polydimethylsiloxane (DVB/CAR/PDMS) (Supelco, Bellefonte, PA, USA) stationary phases were used for the SPME analysis.
Trace 1310 (Thermo Scientific, Waltham, MA, USA) coupled with a mass spectrometer ISQ QD (Thermo Scientific, Waltham, MA, USA) with a 007-5MS column (30 m, 0.25 mm, 0.25 μm) (Quadrex, Woodbridge, CT, USA) were used to analyze the VOC compounds present in the cells of the Calypogeia azurea species.
The TriPlus RSH (Thermo Scientific, Waltham, MA, USA) automatic sample injector was used to ensure that the samples were dispensed with sufficient reproducibility.
HD was carried out using a Deryng apparatus consisting of a 500 mL round-bottom flask, a condenser, and a heating bowl (Lab-szkło, Kraków, Poland), recommended by the VI edition of the Polish Pharmacopoeia of 2002. The Ethos one (Milestone, Sorisole, Italy) was used for MAE.
Fused silica fibers coated with divinylbenzene/carboxy/polydimethylsiloxane (DVB/CAR/PDMS) (Supelco, Bellefonte, PA, USA) stationary phases were used for the SPME analysis.
Trace 1310 (Thermo Scientific, Waltham, MA, USA) coupled with a mass spectrometer ISQ QD (Thermo Scientific, Waltham, MA, USA) with a 007-5MS column (30 m, 0.25 mm, 0.25 μm) (Quadrex, Woodbridge, CT, USA) were used to analyze the VOC compounds present in the cells of the Calypogeia azurea species.
The TriPlus RSH (Thermo Scientific, Waltham, MA, USA) automatic sample injector was used to ensure that the samples were dispensed with sufficient reproducibility.
HD was carried out using a Deryng apparatus consisting of a 500 mL round-bottom flask, a condenser, and a heating bowl (Lab-szkło, Kraków, Poland), recommended by the VI edition of the Polish Pharmacopoeia of 2002. The Ethos one (Milestone, Sorisole, Italy) was used for MAE.
8
Murine Keratinocyte Cell Line Characterization
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The murine keratinocyte cell line PAM212, which was derived from BALB/c mouse skin, was kindly bestowed by Dr. Yuspa (National Institutes of Health, NCI, USA).
Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002).
Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002).
9
Aromatic Compound Acquisition Protocol
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Benzene, toluene, phenol, 3-hydroxyphenylacetate (3-HPA), 4-hydroxyphenylacetate (4-HPA), HgCl2, and CdCl2 of analytical grade were obtained from Merck (Darmstadt, Germany). Benzoate (BA), 3-chlorobenzoate (3-CBA), 4-chlorobenzoate (4-CBA), 3,5-dichlorobenzoate (3,5-CBA), 2-hydroxybenzoate (salicylate), 3-hydroxybenzoate (3-HBA), 4-hydroxybenzoate (4-HBA), 4-isopropylbenzoate (p-cumate), vanillin, cinnamate, L-phenylalanine, phenylacetate, anthranilate, 4-aminobenzoate (pABA), benzamide, nitrobenzene, catechol, gallate, hydroxyquinol (HQ), p-cymene (>98% purity), ethylbenzene, o-xylene, m-xylene, p-xylene, and sodium succinate were obtained from Sigma-Aldrich (St. Louis, MO, USA).
10
Characterization of Aromatic Compounds and Graphene Derivatives
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The aromatic compounds used in this study included benzene (99.5%; Shanghai Lingfeng Chemical Reagent Co. Ltd., China), naphthalene (99%; Sigma-Aldrich), phenanthrene (98%; Sigma-Aldrich), 1-naphthylamine (98%; Sigma-Aldrich), 2-naphthol (99%; Sigma-Aldrich), chlorobenzene (99.0%; Shanghai Lingfeng Chemical Reagent Co. Ltd.), o-dichlorobenzene (99%; J&K Chemical, China), m-dichlorobenzene (97%; Sigma-Aldrich), p-dichlorobenzene (99.5%; Fluka, Switzerland), 1,2,4-trichlorobenzene (99.0%; Fluka), 1,3,5-trichlorobenzene (99%; Supelco, USA), p-chlorophenol (99%; Alfa Aesar, USA), p-chloroaniline (98%; Nanjing Chemical Reagent Co. Ltd., China), toluene (99.5%; Nanjing Chemical Reagent Co. Ltd), o-xylene (98.0%; Supelco), m-xylene (99.5%; Sigma-Aldrich), p-xylene (99.5%; Sigma-Aldrich), 1,3,5-trimethylbenzene (97%; Macklin, China), nitrobenzene (99.5%; Fluka), m-dinitrobenzene (97%; Sigma-Aldrich), and 1,3,5-trinitrobenzene (99.9%; Sigma-Aldrich). GO (99.0%) and RGO (98.9%) were purchased from XFNANO Materials Tech (China). GO was prepared by the modified Hammers method (18 (link), 23 ) and was mainly in the form of nanosheets with a diameter of 0.5 to 5 μm and thickness of 0.8 to 1.2 nm (according to the information provided by the manufacturer). RGO was prepared by the reduction of GO using l -ascorbic acid (48 ). All these materials were used without further purification.
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