Log In Sign up
The largest database of trusted experimental protocols

Quantichrome urea assay kit

Manufactured by BioAssay Systems
Visit Supplier
Sourced in United States

The Quantichrome Urea Assay Kit is a laboratory equipment product that provides a quantitative method for measuring urea concentrations in various samples. The assay is based on a colorimetric reaction and can be used to determine urea levels in biological fluids, cell culture media, and other relevant samples.

Automatically generated - may contain errors

Lab products found in correlation

Accu chek active, by Roche (2 mentions) Creatinine reagent set, by Horiba (2 mentions) Prism 5, by GraphPad (1 mentions) Costar transwell insert, by Corning (1 mentions) Protease inhibitor, by Roche (1 mentions) Dcfda cellular reactive oxygen species detection assay kit, by Abcam (1 mentions) Creatinine serum colorimetric assay kit, by Cayman Chemical (1 mentions) Kim 1 elisa kit, by R&D Systems (1 mentions) Griess reagent, by Merck Group (1 mentions) Spectramax 190, by Molecular Devices (1 mentions) Quantichrome creatinine assay kit, by BioAssay Systems (1 mentions)

Spelling variants of this product

Urea (3 citations)

15 protocols using quantichrome urea assay kit

1

Enzymatic Assay for Polyamine Oxidase CdmS

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The activity of CdmS was determined by measuring the decrease in substrate quantity or the amount of homospermidine formed. The reaction mixture consisting of 50 mM Tris–HCl (pH 8.5), 1 mM NAD+, 1 mM substrate (agmatine, diaminopropane, putrescine, norspermidine, and/or spermidine) and purified CdmS in a final volume of 100 µL was incubated at 65 °C for 1 h. The post-reaction manipulations were performed in the same manner as described previously25. The products of the enzymatic reaction were analyzed by HPLC as described in “Polyamine analysis”. To determine Km and Vmax, purified TtSpeBap and 10 µM MnCl2 were added to the aforementioned reaction mixture to measure the formation of homospermidine. The ureohydrolase activity of TtSpeBap and TtAGR was assayed as described previously26 (link). Urea produced by the reaction was measured using a Quantichrome Urea Assay Kit (BioAssay Systems, CA, USA) according to the manufacturer’s instructions36 (link).
Kobayashi T., Sakamoto A., Hisano T., Kashiwagi K., Igarashi K., Takao K., Uemura T., Furuchi T., Sugita Y., Moriya T., Oshima T, & Terui Y. (2024). Caldomycin, a new guanidopolyamine produced by a novel agmatine homocoupling enzyme involved in homospermidine biosynthesis. Scientific Reports, 14, 7566.
+ Open protocol
+ Expand
2

Measuring Urea Flux in UT-A1 and UT-B Expressing Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDCK cells stably expressing human UT-A1 or UT-B were cultured in DMEM supplemented with 10% fetal bovine serum. To measure urea flux, cells were grown on 12-mm collagen-coated Costar Transwell inserts (0.4-μm pore size, Corning) for 4 days at 37 °C in the presence of 5% CO2 when cells formed a tight monolayer (transepithelial resistance of 1 kΩ/cm2). An assay of UT-facilitated urea flux was performed as previously described [28 (link)]. After removing the DMEM and washing with PBS, PBS containing forskolin (FSK) with or without CB-20 was added to both the apical- and basal-facing surfaces. After 30 min of incubation, the basal-facing solution was replaced by PBS containing 15 mM urea with or without CB-20. Apical fluid samples were collected at 0, 1, 3, 5, 10, 15, 20, 30, 40, 50, and 60 min. The samples were subjected to an assay for urea (Quantichrome Urea Assay Kit; BioAssay Systems, Hayward, CA) according to the kit procedure. Then, the inhibition rate was calculated from the initial slope with GraphPad Prism 5. The experiment was repeated three times. The UT-B-facilitated urea flux assay was performed with the same procedure as the UT-A1-facilitated urea flux assay, except for FSK stimulation.
Li M., Zhao Y., Zhang S., Xu Y., Wang S.Y., Li B.W., Ran J.H., Li R.T, & Yang B.X. (2019). A thienopyridine, CB-20, exerts diuretic activity by inhibiting urea transporters. Acta Pharmacologica Sinica, 41(1), 65-72.
+ Open protocol
+ Expand
3

Arginase Activity Quantification in Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted cells were cultured for 24 hours with or without LPS (100 ng/ml) and IFN-γ (25 ng/ml). Nitrite formation was quantified using the Standard Griess Assay (Molecular Probes, Life Technologies) according to the manufacturer's instructions. Reactive oxygen species (ROS) formation was measured using DCFDA – Cellular reactive oxygen species detection assay kit (abcam) according to the manufacturer's instructions. In the arginase assay, sorted cells were lysed in 0.1 % Triton X-100 containing protease inhibitors (Roche) at 107 cells/ml and incubated at room temperature for 30 minutes. Subsequently, equal volume of 10 mM MnCl2 and 25 mM Tris-HCl (pH 7.5) were added. Following 10 min incubation at 55 °C, equal volume of 0.5 M L-Arginine (pH 9.7) was added. 5 μl of lysates were incubated for 2 hours at 37°C. BioAssay Systems Quantichrome Urea Assay Kit, which detects urea and citrulline equivalently (20 (link)), wasused according to the manufacturer's instructions in order to determine arginase activity.
Bozkus C.C., Elzey B.D., Crist S.A., Ellies L.G, & Ratliff T.L. (2015). Expression of Cationic Amino Acid Transporter 2 (CAT2) Is Required for Myeloid Derived Suppressor Cell-Mediated Control of T Cell Immunity. Journal of immunology (Baltimore, Md. : 1950), 195(11), 5237-5250.
+ Open protocol
+ Expand
4

Cisplatin Liposome Treatment for Murine Tumor Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols
While under anesthesia, female BALB/C mice were shaved, and C26 cells (3 × 105 cells in 50 μL) were injected subcutaneously in the right hind flank. At eight days post-tumor implantation, mice were randomly distributed into treatment groups of 10 animals. Mice were injected by means of the tail vein with cisplatin (6 mg/kg once a week for 2 weeks) or cisplatin liposomes (6 mg/kg once a week for 2 weeks) in approximately 200 μL of solution. Five days post-treatment, blood was collected by submandibular bleeds and analyzed for 19 toxicity markers (UC Davis Comparative Pathology Dept). Blood urea nitrogen levels were quantified with Quantichrome Urea Assay Kit (BioAssay Systems, Hayward, CA). Mice were weighed and tumors measured every other day. The tumor volume was estimated by measuring the tumor volume in three dimension with calipers and calculated using the formula tumor volume = length × width × height. Mice were removed from the study when (i) a mouse lost 15% of its initial weight, (ii) any tumor dimension was >20 mm, or (iii) the mouse was found dead. The mice were followed until day 60 post-tumor inoculation. Statistical analysis was performed using MedCalc 8.2.1.0 for Windows (MedCalc Software, Mariakerke, Belgium). The tumor growth delay was calculated based upon a designated tumor volume of 400 mm3.
Kieler-Ferguson H.M., Chan D., Sockolosky J., Finney L., Maxey E., Vogt S, & Szoka FC J.r. (2017). Encapsulation, controlled release, and antitumor efficacy of cisplatin delivered in liposomes composed of sterol-modified phospholipids. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 103, 85-93.
+ Open protocol
+ Expand
5

Serum and Urinary Biomarkers for Kidney Injury

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum creatinine and blood urea nitrogen were determined using a serum creatinine colorimetric assay kit (Cat# 700460, Cayman Chemical, Ann Arbor MI) and the QuantiChrome urea assay kit (Cat# DIUR-100, BioAssay Systems, Hayward, CA) respectively.21 (link),22 (link) Urinary KIM-1 levels were assessed using the KIM-1 ELISA kit (Cat#MKM100, R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
KILARI S., SHARMA A., ZHAO C., SINGH A., CAI C., SIMEON M., VAN WIJNEN A.J, & MISRA S. (2021). Identification of novel therapeutic targets for contrast induced acute kidney injury (CI-AKI): alpha blockers as a therapeutic strategy for CI-AKI. Translational research : the journal of laboratory and clinical medicine, 235, 32-47.
+ Open protocol
+ Expand
6

iNOS and Arg1 Activity Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
For assaying iNOS activity, BMDM supernatants were mixed at a 1:1 ratio with Griess reagent (Sigma). Absorbance levels were then measured at 543 nm with a SpectraMax 190 (Molecular Devices, Sunnyvale, CA). The nitrite concentration was calculated with a sodium nitrite 22 standard. For assaying Arg1 activity in BMDMs, the Quantichrome Urea Assay Kit (Bioassay Systems, Hayward, CA) was used.
Huang R., Hu Z., Chen X., Cao Y., Li H., Zhang H., Li Y., Liang L., Feng Y., Wang Y., Su W., Kong Z., Melgiri N., Jiang L., Li X., Du J, & Chen Y. (2021). The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis. Advanced Science, 8(19), 2004162.
+ Open protocol
+ Expand
7

Arg1 Activity Quantification via Urea Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intracellular Arg1 activity was assessed by measuring the amount of urea produced via the metabolism of L-arginine by Arg1 according to the manufacturer's directions (Quantichrome Urea Assay Kit, Bioassay Systems, Hayward, CA, USA).
Cho D.I., Kim M.R., Jeong H.Y., Jeong H.C., Jeong M.H., Yoon S.H., Kim Y.S, & Ahn Y. (2014). Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow-derived macrophages. Experimental & Molecular Medicine, 46(1), e70-.
+ Open protocol
+ Expand
8

Measurement of Plasma Creatinine and BUN

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma creatinine and blood urea nitrogen (BUN) were measured using commercially available assay kits according to the manufacturer’s instructions (BioAssay Systems, Hayward, CA; Quantichrome Creatinine Assay Kit, Cat# DICT-500, Lot number CA04A06; Quantichrome Urea Assay Kit, Cat # DIUR-100, Lot number CA02A12) [26 (link)].
Mohamed R., Rafikova O., O’Connor P.M, & Sullivan J.C. (2020). Greater high-mobility group box 1 in male compared with female spontaneously hypertensive rats worsens renal ischemia–reperfusion injury. Clinical science (London, England : 1979), 134(13), 1751-1762.
+ Open protocol
+ Expand
9

Measuring Diabetic Kidney Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood glucose levels were measured 3 days following STZ administration using tail vein blood samples (Accu-Chek Active; Roche Diagnostics, Mannheim, Germany). Mice were housed in metabolic cages to collect urine for subsequent measurement of albumin or creatinine by enzyme-linked immunosorbent assay (ELISA). Creatinine in urine was determined using the Quantichrome Urea Assay Kit (BioAssay System, Hayward, CA, USA). Urine albumin kit was obtained from AssayPro (St. Charles, MO, USA). Plasma GLP-1 was measured using the appropriate ELISA kit in accordance with the manufacturer's instructions (LINCO Research, St. Charles, MO, USA).
Jung G.S., Jeon J.H., Choe M.S., Kim S.W., Lee I.K., Kim M.K, & Park K.G. (2016). Renoprotective Effect of Gemigliptin, a Dipeptidyl Peptidase-4 Inhibitor, in Streptozotocin-Induced Type 1 Diabetic Mice. Diabetes & Metabolism Journal, 40(3), 211-221.
+ Open protocol
+ Expand
10

Measuring Blood Glucose and Urine Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood glucose levels were measured by tail vein prick and glucometer (Accu-Chek Active, Roche Diagnostics, Mannheim, Germany). Creatinine in urine was determined using the Quantichrome Urea Assay Kit (BioAssay System, Hayward, CA, USA). Urine albumin kit was measured using AssayPro (St. Charles, MO, USA).
Jung J., Park W.Y., Kim Y.J., Kim M., Choe M., Jin K., Seo J.H, & Ha E. (2022). 3-Hydroxybutyrate Ameliorates the Progression of Diabetic Nephropathy. Antioxidants, 11(2), 381.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!