DCFH-DA fluorescence staining was used to detect the level of ROS in mycelia. The treated mycelia were incubated in the dark for 30 min with DCFH-DA (final concentration, 10 μM). After washing with PBS buffer, the mycelia were observed using laser confocal microscopy (488 nm [excitation wavelength] and 525 nm [emission wavelength]) to measure ROS levels (72 (link)). Assay kits (BC3595, BC0175, BC0205, and BC0025; Solarbio Science & Technology Co., Ltd.) were utilized to measure H2O2 content, SOD activity, and MDA content, respectively, in treated mycelia.
Bc3595
Manufactured by Solarbio
Sourced in China
The BC3595 is a laboratory centrifuge designed for general sample preparation and separation. It has a maximum speed of 3,595 rpm and a maximum relative centrifugal force (RCF) of 2,400 x g. The centrifuge can accommodate various types of sample tubes and microplates. Its compact size and simple controls make it suitable for use in a range of laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Bc0205, by Solarbio (5 mentions)
Bc0025, by Solarbio (5 mentions)
Bc0175, by Solarbio (4 mentions)
Bc0090, by Solarbio (3 mentions)
Bc0020, by Solarbio (2 mentions)
Bc1290, by Solarbio (2 mentions)
S0053, by Beyotime (1 mentions)
Mak039, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Bc0170, by Solarbio (1 mentions)
Bc1295, by Solarbio (1 mentions)
Spark 10m, by Tecan (1 mentions)
Acetone, by Sinopharm (1 mentions)
Bc0195, by Solarbio (1 mentions)
Bc0095, by Solarbio (1 mentions)
Bc4750, by Solarbio (1 mentions)
Bc1320, by Solarbio (1 mentions)
Hydrogen peroxide assay kit, by Solarbio (1 mentions)
E el 0128c, by Elabscience (1 mentions)
Bc0290, by Solarbio (1 mentions)
21 protocols using bc3595
1
Measuring Oxidative Stress in Mycelia
2
Quantification of Antioxidant Markers in HT-29 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HT-29 cells were seeded in 6-well plates and treated with different stimuli. Then, the collected cells were disrupted with ultrasound and centrifuged at 14,000 rpm for 15 min at 4°C. The supernatant was used for the colorimetric determination of SOD activity (S0101S, Beyotime Biotechnology, China) and H2O2 (BC3595, Solarbio, China) and GSH (S0053, Beyotime Biotechnology, China) contents. The experimental operation was carried out according to the manufacturer’s instructions.
3
Measuring Oxidative Stress in Mycelia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DCFH-DA fluorescence staining was used to detect the level of ROS in mycelia. The treated mycelia were incubated in the dark for 30 min with DCFH-DA (final concentration, 10 μM). After washing with PBS buffer, the mycelia were observed using laser confocal microscopy (488 nm [excitation wavelength] and 525 nm [emission wavelength]) to measure ROS levels (72 (link)). Assay kits (BC3595, BC0175, BC0205, and BC0025; Solarbio Science & Technology Co., Ltd.) were utilized to measure H2O2 content, SOD activity, and MDA content, respectively, in treated mycelia.
4
Nanozyme Peroxide Consumption Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H2O2 consumption of the nanozymes were determined by spectrophotometry. In a classic assay, the final concentrations of nanozyme and H2O2 in the system were 100 μg/mL and 10 mM, respectively. After incubated at 37 °C for different time, samples were taken and the concentration of H2O2 was measured using a hydrogen peroxide content assay kit (Solarbio BC3595).
5
AICAR Tfase Activity Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetyl-CoA (MAK039, Sigma) and H2O2 (BC3595, Solarbio) were measured according to the instructions provided by the manufacturers. AICAR Tfase activity assays were performed as described [63 (link), 64 (link)]. Cells were homogenized in 20 mM HEPES-KOH buffer, pH 7.5, 10 mM KCl, 1.5 mM MgCl2, 1 mM sodium EDTA buffer, 1 mM sodium EGTA buffer, and 1 mM dithiothreitol in the presence of 250 mM sucrose and protease inhibitor cocktail (Roche Diagnostics). Each reaction mixture contained a final concentration of 66 mM Tris-Cl, pH 7.4, 100 mM 10-f-FH4, 50 mM AICAR, and 50 mM KCl. AICAR TFase was assayed using AICAR and 10-f-H2F by following the production of H2F at 298 nm.
6
Quantifying RBC Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A reactive oxygen species (ROS) assay kit (S0033S, Beyotime, Shanghai, China) was used to detect RBC ROS, and a hydrogen peroxide (H2O2) content detection kit (BC3595, Solarbio, Beijing, China) was used to detect the hydrogen peroxide content in RBCs.
7
Plant Antioxidant Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First, 0.1 g of fresh plant leaves were cut, ground with liquid nitrogen, and added to 1 mL of the corresponding extraction solution. After an adequate vortex in an ice-water bath, the supernatant was obtained via centrifugation at 8000× g. Then, the contents of MDA and H2O2 and the enzyme activities of POD and SOD were determined (BC0020; BC3595; BC0090; BC0170, Solarbio, Beijing, China).
8
Hemolymph Oxidative Stress Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hemolymph samples were collected from 48 to 192 h after modeling. The levels of blood ammonia, hydrogen peroxide, superoxide anion, and hydroxyl free radicals were detected using appropriate kits (BC4385, BC3595, BC1290, Solarbio; A018, Nanjing Jiancheng, China) in accordance with the provided instructions. The enzymatic activities and contents of catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) were measured using appropriate kits (A007; A001; A006, Nanjing Jiancheng, China) following the manufacturer’s instructions. Absorbance of all samples was measured using a microplate spectrophotometer (Eon, BioTek, USA).
9
Quantifying H2O2 and O2•- in plants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The H2O2 and O•2− content were measured using a reagent kit (BC3595 and BC1295, Solarbio, Beijing, China) as per the manufacturer’s protocol (Liu et al., 2018 (link)). In brief, the H2O2 content was measured by reacting the extracting solution with 15% NH4OH and 10% TiCl4 and then measuring the absorbance at 410 nm. The extracting solution of O•2− was reacted with p-aminobenzenesulfonamide and N-1-naphthylethylenediamin dihydrochloride, and the absorbance of the reaction mixture was determined at 530 nm. Colorimetry was performed using a microplate reader (Tecan, Spark 10M, Switzerland).
10
Soybean Cold Stress Antioxidant Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The POD and SOD, H2O2, and MDA activity levels were measured under control, 1h and 24h cold stress in soybean leave samples stored at -80°C. The activities of POD and SOD, H2O2 content, and MDA accumulations were examined using a peroxidase kit id: BC0090 (SolarBio, Beijing, China), superoxide dismutase kit id: BC0175 (SolarBio, Beijing, China), H2O2 test kit id: BC3595 (SolarBio, Beijing, China), and MDA test kit id: BC0025 (SolarBio, Beijing, China) according to the manufacturer protocols. All measurements were performed with three independent replicates.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!