R deprenyl hydrochloride

The ¹H, ¹³C, and 2D NMR spectra were recorded on a Varian Mercury 400 MHz spectrometer, Bruker Avance DRX spectrometer at 600 MHz (¹H) and 150 MHz (¹³C) using TMS as an internal standard. The HR-ESI-MS were done using a Bruker Bioapex-FTMS with electrospray ionization. Adsorbents for column Chromatography including Diaion HP-20, Silica gel 60 F254 (0.2 mm, Merck), MN-polyamide-SC-6, and Sephadex™ LH-20. Human recombinant MAO-A and -B were obtained from BD Biosciences. Clorgyline, R-(-)-deprenyl hydrochloride, Kynuramine dihydrobromide, phenelzine sulfate, 4-hydroxyquinoline, K₂HPO₄ buffer, and DMSO were purchased from Sigma.

All animal care and handling was performed according to the directives of the Animal Care and Use Committee of KAIST (Daejeon, Korea) and the Institutional Animal Care and Use Committee of KIST (Seoul, Korea). APPswe/PSEN1dE9 (APP/PS1; stock number 004462, Jackson Laboratory, USA) mice were maintained as hemizygotes by repeated backcrossing with B6C3 F1 mice. Both sexes of 8- to 13-month-old transgenic mice and wild-type littermates were used for study. All experiments were performed with gender- and age-matched controls. For oral administration of selegiline, the mice were provided with water (control) or selegiline solution ad libitum, refreshed every two or three days. The selegiline solution was prepared by dissolving 10 mg of r-(-)-deprenyl hydrochloride (Sigma, USA) in 150 ml drinking water. In this condition, the dose was calculated as 5~10 mg kg^-1 d^-1.