Protein free blocking solution

Manufactured by Agilent Technologies

Sourced in United States

Protein-free blocking solution is a reagent used to prevent non-specific binding in immunoassays and other biochemical applications. It is designed to effectively block non-specific interactions without the use of protein-based components.

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Lab products found in correlation

Zen light edition software, by Zeiss (1 mentions) Lsm 710 t pmt confocal microscope, by Zeiss (1 mentions) Axio zoom v16 and apotome 2, by Zeiss (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Dp71 digital camera, by Olympus (1 mentions) Citrate buffer, by Agilent Technologies (1 mentions) Hematoxylin, by Agilent Technologies (1 mentions) Wash buffer, by Agilent Technologies (1 mentions) Dual endogenous enzyme block, by Agilent Technologies (1 mentions) Envision dual link system hrp, by Agilent Technologies (1 mentions) Axio imager a2 microscope, by Zeiss (1 mentions) Autostainer, by Agilent Technologies (1 mentions)

2 protocols using protein free blocking solution

Histological Analysis of Lung Tissue

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For histological analysis, lungs were inflated with 4% paraformaldehyde or formalin (3.7% formaldehyde) and fixed for 36 h. Fixed paraffin sections were rehydrated, subjected to antigen retrieval, and blocked in Tris-buffered saline (0.1% Triton X-100 containing 1% bovine serum albumin) or protein-free blocking solution (DAKO), and sequentially incubated with specific primary antibodies and biotinylated (DAKO) or Alexa Fluor-conjugated secondary antibodies (Invitrogen, USA). Images were produced with a conventional microscope mounted with a DP71 digital camera (Olympus, Japan), an LSM 710 T-PMT confocal microscope (Carl Zeiss, Germany), and an AXIO Zoom.V16 and ApoTome.2 (Carl Zeiss). Images were processed with equivalent parameters using the ZEN Light Edition software (Carl Zeiss).

Lee Y.S., Lee J.Y., Song S.H., Kim D.M., Lee J.W., Chi X.Z., Ito Y, & Bae S.C. (2020). K-Ras–Activated Cells Can Develop into Lung Tumors When Runx3-Mediated Tumor Suppressor Pathways Are Abrogated. Molecules and Cells, 43(10), 889-897.

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Immunohistochemical Staining of Immune Cells

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Slides were de-paraffinized and re-hydrated in graded concentrations of alcohol before antigen retrieval in citrate buffer, pH 6 (Dako, Carpinteria, CA; Cat#S1699) at 50°C for 10min and rinsed in wash buffer (Dako; Cat#K8007). All staining was done in a Dako Autostainer. Slides were incubated in dual endogenous enzyme block (Dako; Cat#S2003) for 10min, protein-free blocking solution (Dako; Cat#X0909) for 20min, then in primary antibody for 60min. Primary antibodies and dilutions: mouse CD45 (Becton Dickinson Biosciences, San Jose, CA; Cat#550539), 1:400; mouse CD3 (R&D Systems, Minneapolis, MN; Cat#MAB4841), 1:50. Staining was developed as follows: EnVision +Dual Link System HRP (Dako; Cat#K4061) for 30 min and substrate-chromogen (DAB+) Solution (Dako; Cat#K3468) for 5min. Slides were counterstained with hematoxylin (Dako; Ct#S3301) for 10 min. Quantification of cells was performed by counting staining in at least three non-overlapping fields on a Zeiss Axio Imager A2 microscope.

Ramos S.A., Morton J.J., Yadav P., Reed B., Alizadeh S.I., Shilleh A.H., Perrenoud L., Jaggers J., Kappler J., Jimeno A, & Russ H.A. (2021). Generation of functional human thymic cells from induced pluripotent stem cells. The Journal of allergy and clinical immunology, 149(2), 767-781.e6.

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