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2 protocols using ethanol

1

Comprehensive Chemical Reagent Procurement

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Sodium chloride, methanol, ethanol, amino acid standards, acetonitrile, phenyl isothiocyanate, gallic acid, triethylamine, n-hexane, boron trifluoride-methanol, dichloromethane, formic acid, diethyl ether, petroleum ether, sodium hydroxide, potassium hydroxide, hydrochloric acid, and perchloric acid were purchased from ANPEL Laboratory Technologies (Shanghai) Inc. Vitamin B1/B2 and vitamin E were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), mixed standards of fatty acids, cyanidin 3-O-glucoside, anthranone, 1,2,3-trihydroxybenzene, benzoyl-dl-arginine p-nitroanilide hydrochloride, dimethyl sulfoxide, sulfosalicylic acid, trypsin, and phytic acid were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). The purity of chemicals used for High-Performance Liquid Chromatography (HPLC) and Gas Chromatography Mass Spectroscopy (GCMS) complied with the requirement of relevant procedures. All other chemicals were of analytical grade.
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2

Comprehensive Fatty Acid Standard Characterization

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Methanol and n-hexane (all HPLC grade) were obtained from Fisher Scientific. We obtained NaOH (purity ≥ 95%) and pyrogallic acid from Macklin. Ammonium hydroxide (purity ≥ 25%), ethanol (purity ≥ 95%), isopropanol (purity ≥ 99.5%), and acetyl chloride (purity > 99.5%) were acquired from Anpel. Ultra-pure water was prepared using a Milli-Q purification system (Millipore).
In the study, the following standards were used: FA solution GLC 617 from Anpel; FAME solution GLC 674 from Nu-Chek Prep; 37-FAME-Mix from Supelco; iso-and anteiso-FAME individual standards from Larodan; C5:0, C7:0, C9:0, and C19:0 FAME individual standards from Dr. Ehrenstorfer; C18:1 cis-6, C18:1 cis-8, C18:1 cis-11, C18:1 trans-6, and C18:1 trans-11 FA individual standards and C18:2 cis-9,trans-11, and C18:2 trans-10,cis-12 FAME individual standards from Anpel; C22:5 cis-4, cis-7,cis-10,cis-13,cis-16, and C22:5 cis-7,cis-10,cis-13,cis-16,cis-19 FAME individual standards from A ChemTek; C12:1 cis-5, C16:1 cis-7, and C19:1 cis-10 FA individual standards from Sigma-Aldrich; and C10:1 cis-4, C10:1 cis-9, and C10:1 cis-3 FA individual standards from Macklin.
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