Total RNA was isolated using the RNAiso Plus (TaKaRa, Japan) according to the manufacturer’s instructions. cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara, China) and microRNA First-Strand cDNA Synthesis Kit (Sangon Biotech, China). The expression of circRNA and mRNA in cancer samples and cell lines was performed on an ABIPRISMVR 7300 Sequence Detection System (Applied Biosystems). Each reaction was performed in triplicate. Additional file 1 Table S1 lists the specific primers.
Abiprismvr 7300 sequence detection system
Manufactured by Thermo Fisher Scientific
Sourced in China
The ABIPRISMVR 7300 Sequence Detection System is a real-time PCR instrument designed for gene expression analysis and relative quantification. It features a 96-well thermal cycler and a CCD camera-based detection system to monitor fluorescent signals generated during the amplification process.
Automatically generated - may contain errors
3 protocols using abiprismvr 7300 sequence detection system
1
Profiling circRNA and mRNA Expression in Cancer
2
Comprehensive RNA Extraction and Analysis
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Total RNA was isolated via using TRIzol reagent (Takara, Japan) according to the manufacturer's protocol. Reverse transcription was performed by application of PrimeScript RT Reagent Kit (Takara, Japan) and miRNA RT Reagent kit (Accurate Biology, China). Real-time PCR was performed with Accurate Taq PCR reagent kit (Accurate Biology, China) on an ABIPRISMVR 7300 Sequence Detection System (Applied Biosystems). The specific primers are listed in Table S2 .
3
Quantifying Cellular miRNA Expression
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Total RNA from whole-cell lysates was isolated using the TRIzol (TaKaRa, Japan) according to the manufacturer’s protocol. Reverse transcription was performed by using the PrimeScript RT Reagent Kit (Takara, China). Bulge-loop™ miRNA RT-qPCR Primers were applied to determine the level of miRNAs. Quantitative reverse transcription PCR (qRT-PCR) were performed on an ABIPRISMVR 7300 Sequence Detection System (Applied Biosystems). The specific primers are listed in Additional file 1 : Tables S1 and S2.
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