Zg003

Manufactured by Thermo Fisher Scientific

The ZG003 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. No further details about its core function can be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Eif3c, by Santa Cruz Biotechnology (1 mentions) Pcbp2, by Abnova (1 mentions) Anti gadph, by Proteintech (1 mentions) Molecular imager chemidoc xrs, by Bio-Rad (1 mentions) β actin, by Abcam (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Ab20343, by Abcam (1 mentions) Ab219800, by Abcam (1 mentions) Ab3280, by Abcam (1 mentions) A32965, by Thermo Fisher Scientific (1 mentions) Precision plus protein dual color standard, by Bio-Rad (1 mentions)

4 protocols using zg003

Protein Immunoblot Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

eIF4G2 (Bethyl laboratories, A302-239A), CBP80 (Bethyl laboratories, A301-793A), eIF3c (Santa Cruz Biotechnology, sc-74507), PCBP2 (Abnova, H00005094-M05), anti-GADPH (Proteintech Group Inc, 10494-1-AP; or ZG003, Invitrogen), β-actin (Abcam, ab8229), and anti-RPSA antibodies were raised in mouse against full-length His₆-tagged human RPSA protein expressed in E. coli. Western blots were exposed to Kodak X-ray film or visualized via a Bio-Rad ChemiDoc XRS+ Molecular Imager.

Smirnova V.V., Shestakova E.D., Bikmetov D.V., Chugunova A.A., Osterman I.A., Serebryakova M.V., Sergeeva O.V., Zatsepin T.S., Shatsky I.N, & Terenin I.M. (2019). eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loop. RNA, 25(7), 757-767.

+ Open protocol

+ Expand

Western Blotting Analysis of Ric-8 Proteins

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal antiserum 2413 (used at 1:5000) against Ric-8B and 1184 (used at 1:10,000) against Ric-8A were described previously.^{12 (link),22 (link)} G protein subunit antiserum was used to detect Gβ1–4 (used at 1:10,000) (B600).^{56 (link)} Commercial IgG purified polyclonal antibodies for Gαs (Sigma-Millipore) (used at 1:2000), and Gαq/11 (Sigma-Millipore) (used at 1:2500) were utilized for measuring G protein abundance. A commercial IgG purified monoclonal antibody for was used to detect GAPDH (Invitrogen, ZG003) for loading controls. Primary antibodies for phospho-site detection were described previously.^{34 (link)} Briefly, the Phospho-CK2 Substrate [(pS/pT)DXE] MultiMab^™ Rabbit mAb mix (#8738, Cell Signaling Technology) was used to detect pThr473 in Ric-8B (pThr440 in Ric-8A). The enriched IgG fraction of rabbit antiserum (6383) specifically detects pSer468 in Ric-8B (pSer435 in Ric-8A).^{34 (link)}

Papasergi-Scott M.M., Kwarcinski F.E., Yu M., Panova O., Ovrutsky A.M., Skiniotis G, & Tall G.G. (2023). Structures of Ric-8B in complex with Gα protein folding clients reveal isoform specificity mechanisms. Structure (London, England : 1993), 31(5), 553-564.e7.

+ Open protocol

+ Expand

Detecting Protein Expression in Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

For detection of protein expression in lung tissue, samples were lysed in a 1% SDS buffer (1% SDS, 50mM triethanolamine pH 7.4, 150mM NaCl) containing a cocktail of phosphatase protease inhibitors (Sigma, 4906845001) and protease inhibitors (Thermo Scientific, A32965). The lysates were centrifuged at 15,000 rpm for 10 min and soluble protein supernatents were used for Western blot analysis. Equal amounts of protein (30ug) were separated by SDS-PAGE and transferred onto membranes. Membranes were blocked with 10% non-fat milk in Phosphate-buffered saline with 0.1% Tween-20 (PBST) and probed with antibodies against GSDMD (abcam, ab219800) and actin (abcam, ab3280). For Western blotting of THP-1 cells, samples were lysed in 1% SDS containing protease inhibitors prior to SDS-PAGE separation as described above. Membranes were probed with antibodies against influenza virus nucleoprotein (abcam, ab20343), GSDMD (abcam, ab210070), and GAPDH (Thermo Scientific, ZG003).

Speaks S., Zani A., Solstad A., Kenney A., McFadden M.I., Zhang L., Eddy A.C., Amer A.O., Robinson R., Cai C., Ma J., Hemann E.A., Forero A, & Yount J.S. (2023). Gasdermin D promotes influenza virus-induced mortality through neutrophil amplification of inflammation. bioRxiv.

+ Open protocol

+ Expand

Quantitative Analysis of p53 and p21 Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

For protein analysis of p53 and p21/Waf1 expression, whole cell extracts were prepared from 1–5 x10⁶ cells lysed in 30–50 μL of buffer containing 10 мМ Tris-HCl pH 7.4, 0.1% Triton X-100, 5 мМ PMSF, 5 мМ MgCl₂, 5 u/mL DNAse I, 20 мМ β-mercaptoethanol, followed by sonication. 20 μg of total protein, as estimated by Bradford assay (Pierce), were resolved by 10% SDS–PAGE and transferred to a PVDF membrane. These were hybridised with either mouse monoclonal p53 antibody (clone DO-1, Sigma) at a 1:1,000 dilution, or p21/Waf1 (clone CP74, Millipore) at a 1:1,000 dilution followed by treatment with the peroxidase-labeled anti-mouse IgG (whole molecule) antibody (Sigma) at a 1:10,000 dilution. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was utilised as an endogenous control for equal loading using the monoclonal antibody ZG003 (ThermoFisher Scientific) at a 1:10,000 dilution. Antibody binding was detected by enhanced chemoluminescence (Pierce). Precision Plus Protein Dual Color standards (Bio-Rad) were used to estimate the molecular weight of the detected proteins.

Makarov E.M., Shtam T.A., Kovalev R.A., Pantina R.A., Varfolomeeva E.Y, & Filatov M.V. (2017). The rare nonsense mutation in p53 triggers alternative splicing to produce a protein capable of inducing apoptosis. PLoS ONE, 12(9), e0185126.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!