Dimethyl sulfoxide (DMSO) was purchased from Nacalai Tesque (Kyoto, Japan), fingolimod (FTY720) from Selleck Chemicals (Houston, TX, USA), and 6α-methylprednisolone, propanolol and verapamil from Sigma Aldrich (Tokyo, Japan).
6α methylprednisolone
Manufactured by Merck Group
Sourced in United States
6α-methylprednisolone is a synthetic glucocorticoid medication used in the treatment of various inflammatory and autoimmune conditions. It is a derivative of the natural hormone prednisolone, with a methyl group added at the 6α position.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Nacalai Tesque (1 mentions)
Fingolimod fty720, by Selleck Chemicals (1 mentions)
Verapamil, by Merck Group (1 mentions)
Propanolol, by Merck Group (1 mentions)
Albuterol sulfate, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Rifampicin, by Merck Group (1 mentions)
Ru486, by Merck Group (1 mentions)
Sr12813, by Merck Group (1 mentions)
Hsa mir 18a 5p, by Qiagen (1 mentions)
Hsa mir 34a 5p, by Qiagen (1 mentions)
Pregnenolone 16α carbonitrile, by Merck Group (1 mentions)
Corticosterone, by Merck Group (1 mentions)
Cel mir 39 3p, by Qiagen (1 mentions)
Triamcinolone acetonide, by Merck Group (1 mentions)
Hydrocortisone acetate, by Merck Group (1 mentions)
Phenobarbital pb, by Merck Group (1 mentions)
Cortisone, by Merck Group (1 mentions)
4 protocols using 6α methylprednisolone
1
Pharmacological Agents for Research
2
Pulmonary Function Assessment in MWCNT-Exposed Mice
3
Ligands and Inhibitors for Nuclear Receptors
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Rifampicin (a prototypical ligand of the human PXR, Rif), SR-12813 (a human PXR ligand), and phenobarbital (PB) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Dexamethasone (DEX) (a prototypical ligand of GR), RU486 (mifepristone, GR antagonist), DMSO (dimethyl sulfoxide), pregnenolone 16α-carbonitrile (PCN, an agonist of mouse PXR), hydrocortisone acetate, corticosterone, triamcinolone acetonide, and 6α-methylprednisolone were purchased from Sigma–Aldrich. Stock solutions (1000×) were prepared in DMSO prior to dilution in cell culture medium. The final concentration of DMSO in the media did not exceed 0.2% (v/v) in any experiments. SPA70, an PXR inhibitor, has been purchased from abcr GmbH Im (Karlsruhe, Germany)25 (link).
miRCURY LNA microRNA mimics cel-miR-39-3p (mimic control, 479902-001), hsa-miR-18a-5p (471717-001), hsa-miR-148a-3p (339173 YM00472598-ADA), hsa-miR-34a-5p (339173 YM00473212-ADA), hsa-miR-449a (339173 YM00473262-ADA), and miRCURY LNA inhibitors, such as negative control A (inhibitor control, 199006-001), hsa-miR-18a-5p (4100723-001), and hsa-miR-34a-5p (339121 YI04100982-ADA), were obtained from Exiqon (now part of QIAGEN, Germantown, MD, USA).
miRCURY LNA microRNA mimics cel-miR-39-3p (mimic control, 479902-001), hsa-miR-18a-5p (471717-001), hsa-miR-148a-3p (339173 YM00472598-ADA), hsa-miR-34a-5p (339173 YM00473212-ADA), hsa-miR-449a (339173 YM00473262-ADA), and miRCURY LNA inhibitors, such as negative control A (inhibitor control, 199006-001), hsa-miR-18a-5p (4100723-001), and hsa-miR-34a-5p (339121 YI04100982-ADA), were obtained from Exiqon (now part of QIAGEN, Germantown, MD, USA).
4
Quantitative Analysis of Cortisol and Cortisone
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Analytical 98 % purity cortisol (CAS No. 50-23-7) and cortisone (CAS No. 53-06-5), and internal standard 6α-methylprednisolone (CAS No. 83-43-2) were the products of Sigma-Aldrich® (St. Louis, MO, USA). Solvents LiChrosolv® methanol and LiChrosolv® acetonitrile were supplied by J. T. Baker® (Deventer, the Netherlands), while ChromaSolv™ Plus acetone and SupraSolv® diethyl ether were supplied by Merck® (Darmstadt, Germany). All solvents were of HPLC or gas chromatography (GC) analytical grade. Ultrapure water was obtained with a Millipore® purification system (Bedford, MA, USA).
Individual stock solutions (1 mg/mL) were prepared in methanol and stored at −18 °C. Calibration standards, containing a mixture of cortisol and cortisone with concentrations of both molecules in the 50–500 nmol/L range, and the internal standard with constant concentration at 500 nmol/L were prepared from stock solutions diluted in methanol:water (1:1, v/v) right before analysis. A spike solution containing cortisol and cortisone for method efficiency test as well as the internal standard solution for spiking the real and fortified saliva samples were prepared from stock solutions further diluted in water. The internal standard solution in water was kept at −18 °C for no longer than two months.
Individual stock solutions (1 mg/mL) were prepared in methanol and stored at −18 °C. Calibration standards, containing a mixture of cortisol and cortisone with concentrations of both molecules in the 50–500 nmol/L range, and the internal standard with constant concentration at 500 nmol/L were prepared from stock solutions diluted in methanol:water (1:1, v/v) right before analysis. A spike solution containing cortisol and cortisone for method efficiency test as well as the internal standard solution for spiking the real and fortified saliva samples were prepared from stock solutions further diluted in water. The internal standard solution in water was kept at −18 °C for no longer than two months.
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