Log In Sign up
The largest database of trusted experimental protocols

Vs 120 slide scanner

Manufactured by Hamamatsu Photonics
Visit Supplier
Sourced in Germany

The VS 120 Slide Scanner is a high-resolution digital slide scanning system designed for a variety of applications. The device captures detailed images of microscope slides, enabling efficient digitization of biological samples for further analysis and archiving.

Automatically generated - may contain errors

Lab products found in correlation

Orca flash 4.0 camera, by Hamamatsu Photonics (2 mentions) Cresyl violet, by Merck Group (1 mentions) Permount, by Thermo Fisher Scientific (1 mentions) Digital camera c11440, by Olympus (1 mentions) Axiovert 200m, by Zeiss (1 mentions) Csu x1, by Yokogawa (1 mentions) C9100 23b em ccd camera, by Hamamatsu Photonics (1 mentions) Orca r2 camera, by Hamamatsu Photonics (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions) Insulin, by Agilent Technologies (1 mentions)

Spelling variants of this product

Slide scanner (39 citations)

5 protocols using vs 120 slide scanner

1

Automated Immunofluorescent Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Automated imaging of immunofluorescent sections stained as described above (“Immunohistochemical and immunofluorescent assay”) with Ki67 (labeled with Alexa Fluor 488 secondary), Cldu (labeled with Alexa Fluor 568), and DAPI staining. Images were acquired on an Olympus VS 120 Slide Scanner with a Hamamatsu ORCA-Flash 4.0 camera and a 40× 0.65 NA objective. Filter cubes were standard Olympus DAPI, FITC (for Alexa Fluor 488), and TRITC (for Alexa Fluor 568) cubes. Exposure times and illumination intensities were kept constant across time points and replicates.
He X., Smith S.E., Chen S., Li H., Wu D., Meneses-Giles P.I., Wang Y., Hembree M., Yi K., Zhao X., Guo F., Unruh J.R., Maddera L.E., Yu Z., Scott A., Perera A., Wang Y., Zhao C., Bae K., Box A., Haug J.S., Tao F., Hu D., Hansen D.M., Qian P., Saha S., Dixon D., Anant S., Zhang D., Lin E.H., Sun W., Wiedemann L.M, & Li L. (2021). Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell reports, 36(10), 109674.
+ Open protocol
+ Expand
2

Cresyl Violet Staining and Brightfield Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sections were stained in 0.1% Cresyl violet solution (Cresyl violet, Sigma Cat# C5042) for 10 min, rinsed quickly in distilled water, dehydrated in 95% ethanol, and left in xylene before being cover-slipped with Permount (Thermo Fischer Scientific, SP15-500). Brightfield images were taken in the Olympus VS-120 slide-scanner with Hamamatsu digital camera C11440 and processed using the Olympus VS-Olyvia 2.9 software.
Roy A., Murphy R.M., Deng M., MacDonald J.W., Bammler T.K., Aldinger K.A., Glass I.A, & Millen K.J. (2019). PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice. eLife, 8, e45961.
+ Open protocol
+ Expand
3

Fluorescence Imaging of Cellular Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence imaging was performed on an Olympus VS120 slide scanner, equipped with an X-Cite 120 LED light source and a Hamamatsu Flash 4.0 sCMOS camera or a spinning-disk confocal microscope (UltraVIEW; PerkinElmer), comprising an inverted stand (Axiovert 200 M; Carl Zeiss Microimaging, Jena, Germany) and a spinning-disk confocal head (CSU-X1; Yokogawa Corporation of America) with Velocity acquisition software (PerkinElmer) and dual cameras: a Hamamatsu C9100-23B EMCCD camera and a Hamamatsu Orca-R2 camera. Fluorescence channels were acquired sequentially. DAPI fluorescence was acquired with a 405 nm diode laser and a 445 (W60), 615 (W70) dual bandpass emission filter. Red (Alexa 546) fluorescence a 561 nm diode laser and a 445 (W60), 615 (W70) dual bandpass emission filter.
Tao F., Soffers J., Hu D., Chen S., Gao X., Zhang Y., Zhao C., Smith S.E., Unruh J.R., Zhang D., Tsuchiya D., Venkatraman A., Zhao M., Li Z., Qian P., Parmely T., He X.C., Washburn M., Florens L., Perry J.M., Zeitlinger J., Workman J, & Li L. (2020). β-Catenin and Associated Proteins Regulate Lineage Differentiation in Ground State Mouse Embryonic Stem Cells. Stem Cell Reports, 15(3), 662-676.
+ Open protocol
+ Expand
4

Automated Immunofluorescent Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Automated imaging of immunofluorescent sections stained as described above (“Immunohistochemical and immunofluorescent assay”) with Ki67 (labeled with Alexa Fluor 488 secondary), Cldu (labeled with Alexa Fluor 568), and DAPI staining. Images were acquired on an Olympus VS 120 Slide Scanner with a Hamamatsu ORCA-Flash 4.0 camera and a 40× 0.65 NA objective. Filter cubes were standard Olympus DAPI, FITC (for Alexa Fluor 488), and TRITC (for Alexa Fluor 568) cubes. Exposure times and illumination intensities were kept constant across time points and replicates.
He X., Smith S.E., Chen S., Li H., Wu D., Meneses-Giles P.I., Wang Y., Hembree M., Yi K., Zhao X., Guo F., Unruh J.R., Maddera L.E., Yu Z., Scott A., Perera A., Wang Y., Zhao C., Bae K., Box A., Haug J.S., Tao F., Hu D., Hansen D.M., Qian P., Saha S., Dixon D., Anant S., Zhang D., Lin E.H., Sun W., Wiedemann L.M, & Li L. (2021). Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche. Cell reports, 36(10), 109674.
+ Open protocol
+ Expand
5

Pancreatic Insulin Immunostaining Post-RYGB FMT

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pancreatic tissues from pre- and post-RYGB FMT mice were fixed for 24 hours in 4% paraformaldehyde in PBS (pH 7.4). Paraffin sections (7 μm thick) were obtained separately from pancreas and stained for insulin (Dako Canada) (catalog IR00261-2). insulin-immunostained sections were scanned using an Olympus VS120 slide scanner and analyzed with NDP.view2 plus software from HAMAMATSU. insulin immunostaining was calculated by an automated positive pixel count algorithm to determine total β-cell area, islet number per total pancreatic area, and islet size.87 (link)
Yadav J., Liang T., Qin T., Nathan N., Schwenger K.J., Pickel L., Xie L., Lei H., Winer D.A., Maughan H., Robertson S.J., Woo M., Lou W., Banks K., Jackson T., Okrainec A., Hota S.S., Poutanen S.M., Sung H.K., Allard J.P., Philpott D.J, & Gaisano H.Y. (2023). Gut microbiome modified by bariatric surgery improves insulin sensitivity and correlates with increased brown fat activity and energy expenditure. Cell Reports Medicine, 4(5), 101051.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!