Unity 500 mhz

Manufactured by Agilent Technologies

Sourced in United States

The Unity 500 MHz is a high-performance nuclear magnetic resonance (NMR) spectrometer produced by Agilent Technologies. It operates at a frequency of 500 MHz and is designed for advanced analytical applications in various fields, including chemistry, materials science, and life sciences.

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Lab products found in correlation

L aspartic acid, by Merck Group (1 mentions) Dmso d6, by Cambridge Isotopes (1 mentions) Cdcl3, by Cambridge Isotopes (1 mentions) 996 photodiode array detector, by Waters Corporation (1 mentions) Finnigan ltq fticr ms instrument, by Thermo Fisher Scientific (1 mentions) 515 hplc pump, by Waters Corporation (1 mentions) Milli q system, by Merck Group (1 mentions) Voyager de str, by Thermo Fisher Scientific (1 mentions) Waters synapt hdms lc ms mass spectrometer, by Waters Corporation (1 mentions) Uv 1650pc spectrophotometer, by Shimadzu (1 mentions) Ft ir 4100 spectrophotometer, by Jasco (1 mentions) J 1500 spectrometer, by Jasco (1 mentions) Autopol 3 polarimeter, by Rudolph Research Analytical (1 mentions)

Close spelling variants of this product

Unity Inova 500 MHz instrument Varian Unity Inova 500 MHz NB High-Resolution FT NMR Unity Inova 500 MHz spectrometer 500 MHz Unity-Plus spectrometer Unity 500 MHz spectrometer Unity INOVA 500 MHz (1H) spectrometer Unity Inova 500 MHz NMR spectrometer Unity Inova 500 MHz Unity Inova 500 MHz NB High Resolution FT NMR Unity 500

5 protocols using unity 500 mhz

Synthesis and Characterization of Poly(Succinimide) Derivatives

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PSI was synthesized
by acid-catalyzed
polycondensation of l-aspartic acid (Sigma, MO).^{1 (link)} Designated amounts of 2-AEMA (Sigma) and ethanolamine
were added sequentially to PSI dissolved in dimethylformamide. The
molar ratio of 2-AEMA was adjusted to regulate the DS of MA groups
(DS_MA) from 0 to 10%. The molar ratio of ethanolamine was
adjusted to regulate the DS of hydroxyl groups from 95 to 85%, respectively.
The molar amounts of the reagents used for the reactions are listed in Table 2.
The
resulting polymer was dissolved in DMSO-d₆ (Cambridge Isotope Laboratories, Inc.) and analyzed by ¹H NMR (Varian Unity 500 MHz). DS_MA and DS_OH were calculated from the integrated peaks of the ¹H NMR
spectra using the following equations^{2 (link)}

Park J., Yu Y., Kim J., Qin E.C., Kim M.J., Ko E, & Kong H. (2017). Balanced Effects of Surface Reactivity and Self-Association of Bifunctional Polyaspartamide on Stem Cell Adhesion. ACS Omega, 2(4), 1333-1339.

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Comprehensive NMR and Mass Spectrometry Analysis

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NMR spectra were collected on a Varian Unity 500 MHz (500 MHz and 125 MHz for the ¹H and ¹³C nuclei respectively), a Varian VX 500 MHz with ¹³C-optimized cryoprobe, and a Bruker 600 MHz (600 MHz and 150 MHz for the ¹H and ¹³C nuclei respectively) with 1.7 mm inverse cryo-probe. NMR experiments were conducted using CDCl₃ from Cambridge Isotope Laboratories, Inc. 99.8% D, containing 0.03% or 1.0% v/v trimethylsilane (referencing δ_H 0.0 as the internal standard from trimethylsilane, and δ_C 0.0 or δ_C 77.16 as internal standards using trimethylsilane and CDCl₃, respectively). Microwave-heated reactions were run in a Biotage Initiator microwave synthesizer. LR-LCMS data were collected on a Thermo Finnigan Surveyor Autosampler/LC-Pump-Plus/PDA-Puls with a Thermo Finnigan Advantage Max mass spectrometer. HRMS data was collected on a Finnigan LTQ-FTICR-MS instrument (Thermo-Electron Corporation, San Jose, CA) fitted with either an Ion-Max ESI source for LCMS runs, or a Biversa Nanomate (Advion Biosystems, Ithaca, NY) electrospray source. HPLC purification was carried out with a Waters 515 HPLC Pump with a Waters 996 Photodiode Array Detector using Empower Pro software. All solvents were HPLC grade except for H₂O which was purified with a Millipore Milli-Q system before use, CH₃CN which was LCMS grade from J.T. Baker, and acetone which was distilled before use.

Boudreau P.D., Monroe E.A., Mehrotra S., Desfor S., Korobeynikov A., Sherman D.H., Murray T.F., Gerwick L., Dorrestein P.C, & Gerwick W.H. (2015). Expanding the Described Metabolome of the Marine Cyanobacterium Moorea producens JHB through Orthogonal Natural Products Workflows. PLoS ONE, 10(7), e0133297.

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NMR and Mass Spectrometry Analysis

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¹H NMR spectra were acquired using Varian Unity 500 MHz (¹³C, 126 MHz) spectrometer. ¹H NMR chemical shifts (δ) are reported in parts per million (ppm) and were referenced to the residual solvent peak at 7.26 ppm for CDCl₃ and 2.50 ppm for DMSO-d₆. ¹³C NMR chemical shifts are reported in ppm and were referenced to the residual solvent peak at 77.16 ppm for CDCl₃ and 39.52 ppm for DMSO-d₆. All NMR spectra were original one which was scanned. Mass spectra were obtained on Micromass Q-Tof Ultima (HR-ESI) and Micromass Quattro (LR-ESI) instruments. MALDI-TOF-MS was carried out using Applied Biosystems Voyager-DE STR with a nitrogen laser (337 nm, 3 ns pulse, 20 Hz maximum firing rate) and using 2-(4′-hydroxybenzeneazo)benzoic acid (HABA) as matrix.

Wang L., Yin Z., Zhang Y., Jiang Y., Zhang L, & Yasin A. (2018). Probing the single pair rupture force of supramolecular quadruply hydrogen bonding modules by nano-adhesion measurement. RSC Advances, 8(39), 21798-21805.

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Comprehensive Spectroscopic Analysis of Compounds

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NMR spectra (¹H, ¹³C, COSY, HSQC, HMBC and NOESY) were measured on a Varian Unity 500 MHz (Varian Inc., Palo Alto, CA, USA) and a Bruker 600 MHz (Bruker BioSpin GmbH, Rheinstetten, Germany) spectrometer with TMS as an internal standard. Optical rotations were obtained on a Rudolph Research Analytical Autopol III polarimeter (Rudolph Research Analytical, Hackettstown, NJ, USA). CD spectra were recorded on a JASCO J-1500 spectrometer (JASCO Corporation, Tokyo, Japan). UV spectra were acquired on a Shimadzu UV-1650PC spectrophotometer (Shimadzu Corporation, Kyoto, Japan). IR spectra were measured on a JASCO FT/IR-4100 spectrophotometer (JASCO Corporation, Tokyo, Japan). HRESIMS were measured on Waters Synapt HDMS LC/MS mass spectrometer (Waters Corporation, Milford, MA, USA). HPLC system was composed of a PrimeLine Binary pump (Analytical Scientific Instruments, Inc., El Sobrante, CA, USA) with RI-101 (Shoko Scientific Co. Ltd., Yokohama, Japan), semi-preparative ODS column (YMC-Pack-ODS-A, 250 × 10 mm i.d, 5 µm) and an analytical ODS column (YMC-Pack-ODS-A, 250 × 4.6 mm i.d, 5 µm)(YMC Corporation, Kyoto, Japan).

Choi B.K., Jo S.H., Choi D.K., Trinh P.T., Lee H.S., Anh C.V., Van T.T, & Shin H.J. (2020). Anti-Neuroinflammatory Agent, Restricticin B, from the Marine-Derived Fungus Penicillium janthinellum and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells. Marine Drugs, 18(9), 465.

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Synthesis and Characterization of Organic Compounds

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1, 4-dioxane aqueous, kieselguhr, anhydrous ethanol, anhydrous acetonitrile, sodium carbonate, acetone, sodium hydroxide, and hydrochloric acid were purchased from SCRC. Bathocuproine (BC), selenium dioxide, sodium borohydride, and hydrobromic acid was purchased from ABCR. Diethyl iminodiacetat was purchased from Sigma. Anhydrous ethanol was distilled from magnesium and iodine. Anhydrous acetonitrile was distilled from phosphorus pentoxide.
¹H NMR spectra were recorded on Varian Unity+ 500M Hz. ¹H NMR spectra were registered in D₂O, and chemical shifts are expressed in partsper million (δ) relative to internal Me₄Si. IR spectra were recorded on a Nicolet Avatar 330 FTIR spectrophotometer. Mass spectra were recorded by Bruke BioTOF-Q. UV spectra were recorded on a Persee TU-1900 ultraviolet and visible spectrophotometer.

Shen Y., Xu S, & He D. (2015). A Novel Europium Chelate Coated Nanosphere for Time-Resolved Fluorescence Immunoassay. PLoS ONE, 10(6), e0129689.

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