Malondialdehyde mda

Paroxetine (PAR) was purchased from the National institutes for Food and Drug Control (Beijing, China). The standard substances of dopamine (DA), 5-hydroxytryptamine (5-HT), norepinephrine (NE), epinephrine (E) and acetylcholine (Ach) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Corticosterone (CORT) and adrenocorticotrophic hormone (ACTH), total antioxidant capacity (T-AOC) assay kit (FRAP method), malondialdehyde (MDA) and superoxide dismutase (SOD) were purchased from Nanjing Jiancheng Biotechnology Institute (Nanjing, China). Acetonitrile and ammonium formate of HPLC grade were obtained from Thermo Fisher Scientific (MA, USA). Ultrapure water was obtained from a Milli-Q water purification system (Millipore, USA). All other chemicals used for analysis were analytical grade, obtained from Beijing Chemical Works (Beijing, China).
Fourteen reference compounds for UPLC-DAD/Q-TOF MS analysis were isolated from okra seeds by our research group. Their structures were identified by ¹³C NMR, ¹H NMR and HR-MS, and the purities determined with HPLC method were all over 95%. Their structures were shown in ESI 1.†

RSL3 (#HY-100218A) and Fer-1 (#HY-100579) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA). Sodium butyrate (NaB, #S1999) was purchased from Selleck Chemicals, Houston, TX, USA. Dimethyl sulfoxide (DMSO, Solarbio, Beijing, China, #D8371) was used to prepare the stock solution of drugs. FFAs was purchased from Shanghai Keshun Science and Technology co., Ltd. (Shanghai, China). Fetal Bovine Serum (FBS, # FSD500) was purchased from Excell Bio. DCFH-DA (#S0033S) and DAPI (#C1002) were purchased from Beyotime Bio (Shanghai, China). Alanine aminotransferase (ALT, #C009-2-1), aspartate aminotransferase (AST, #C010-2-1), total cholesterol (TC, #A111-1-1), triglyceride (TG, #A110-1-1), malondialdehyde (MDA, #A003-4-1), glutathione peroxidase (GSH-pX, #A005-1-2), and glutathione (GSH, #A006-2-1) were purchased from Nanjing Jiancheng Bio (Nanjing, China). Transferrin receptor (TFRC, # bs-41331R), ferritin heavy chain 1 (FTH1, #bs-5907R), nuclear receptor coactivator 4 (NCOA4, #bs-19051R), cyclooxygenase-2 (COX-2, #bs-10411R), GPX4 (#bs-3884R), and GAPDH (#bs-41373R) were purchased from Bioss Bio (Beijing, China). Solute carrier family 7 member 11 (SLC7A11 or xCT, #26864-1-AP) was purchased from Proteintech Bio (Rosemont, IL, USA).

Superoxide dismutase (SOD) and Malondialdehyde (MDA) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). CCl₄ solution was purchased from Adamas (Shanghai, China). Colchicine was purchased from Xishuangbanna Pharmaceutical Co., Ltd. (Jinghong, China). Olive oil was purchased from Macklin (Shanghai, China).