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Radioimmunoassay

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Sourced in Japan, United States

Radioimmunoassay is a sensitive analytical technique used to quantify the concentration of specific substances, such as hormones, drugs, or other biomolecules, in a sample. It utilizes the high specificity of antibodies and the radioactive properties of certain isotopes to detect and measure the target analyte.

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Lab products found in correlation

Second generation enzyme immunoassay, by Abbott (7 mentions) Modular analytics system, by Roche (3 mentions) Elecsys hbsag 2 quant assay, by Roche (3 mentions) E plate test, by Eiken Chemical (2 mentions) Lz test, by Eiken Chemical (2 mentions) E plate, by Eiken Chemical (2 mentions) Chemiluminescent immunoassay, by Abbott (2 mentions) Cobas amplicor, by Roche (2 mentions) Cobas taqman, by Roche (2 mentions) Model 736, by Hitachi (2 mentions) Axsym, by Abbott (1 mentions) Cleia, by Fujirebio (1 mentions) Hiscl 5000, by Sysmex (1 mentions) Third generation enzyme linked immunosorbent assay, by Abbott (1 mentions) Cobas amplicor hbv monitor test kit, by Roche (1 mentions)

15 protocols using radioimmunoassay

1

Gastrin, Pepsinogens, and Helicobacter Pylori Evaluation

Cited 1 time
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Fasting blood samples were collected, and serum samples were stored at –20 °C until further use for both groups. Serum gastrin concentrations were measured using the Gastrin RIA Kit II (Dynabot, Tokyo, Japan). Serum PG I and PG II concentrations were determined by radioimmunoassay (Abbott, Tokyo, Japan) for the years 1990 to 1999, chemiluminescent immunoassay (Abbott) from 1999 to 2001, enzyme immunoassay (E-plate test; Eiken, Tokyo, Japan) from 2001 to 2003, and latex agglutination test (L-Z test; Eiken) from 2003 to 2014. PG positivity was defined as serum PG I concentrations of ≤ 70 ng/mL and a PG I/II ratio of ≤ 3.0 [31 –33 (link)]. Serum anti-Hp Abs in the pathology group was determined with the Pyloristat kit (Whittaker Bioproducts, Walkerville, MD, USA) using enzyme-linked immunosorbent assay (ELISA). Serum anti-Hp Abs titers of the endoscopically-evaluated group were evaluated using ELISA (E-plate; Eiken Chemical). The cut-off value for anti-Hp Abs in the endoscopically-evaluated group was 3 U/mL [34 (link), 35 (link)].
Nagasaki N., Takigawa H., Ito M., Boda T., Kotachi T., Hayashi R., Yuge R., Urabe Y., Oka S, & Tanaka S. (2023). Diagnostic performance of the normal range of gastrin calculated using strict criteria based on a combination of serum markers and pathological evaluation for detecting gastritis: a retrospective study. BMC Gastroenterology, 23, 167.
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2

Serum Biomarker Assessment Protocol

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Serum hepatitis B surface antigen (HBsAg) and the HCV antibody were tested by radio-immunoassay (Abbott Laboratories, North Chicago, IL) and second-generation enzyme immunoassay (Abbott). Serum biochemistries were measured using a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany). The serum AFP level was tested using a radio-immunoassay kit (Serono Diagnostic SA, Coinsin/VD, Switzerland).
Hsieh W.Y., Chen P.H., Lin I.Y., Su C.W., Chao Y., Huo T.I., Huang Y.H., Hou M.C., Lin H.C, & Wu J.C. (2017). The impact of esophagogastric varices on the prognosis of patients with hepatocellular carcinoma. Scientific Reports, 7, 42577.
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3

Viral Hepatitis Serological Assays

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HBsAg was measured by enzyme immunoassay (AxSYM; Abbott Japan, Tokyo, Japan) and chemiluminescence enzyme immunoassay (CLEIA) (Fujirebio, Tokyo, Japan). The IgG class of anti-HBc was determined by radioimmunoassay (Abbott Japan). Anti-HBs was tested by enzyme immunoassay (AxSYM; Abbott Japan, Tokyo Japan). Anti-HCV was tested by CLEIA (Fujirebio, Tokyo, Japan). All serologic assays were performed according to the manufacturer's instructions.
Elkady A., Iijima S., Aboulfotuh S., Mostafa Ali E., Sayed D., Abdel-Aziz N.M., Ali A.M., Murakami S., Isogawa M, & Tanaka Y. (2017). Characteristics of escape mutations from occult hepatitis B virus infected patients with hematological malignancies in South Egypt. World Journal of Hepatology, 9(9), 477-486.
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4

Biomarkers for Hepatic Steatosis Assessment

Cited 2 times
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Venous blood samples were collected after an overnight fast. Radio-immunoassay (Abbott Laboratories, North Chicago, IL, USA) was used to test serum HBV surface antigen (HBsAg), and second-generation enzyme immunoassay (Abbott Laboratories) was used to test antibody to HCV (anti-HCV). The Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany) was utilized to measure serum biochemical markers. FLI was calculated using the following formula: FLI = (e 0.953*loge (TG) + 0.139*BMI + 0.718*loge (GGT) + 0.053*WC—15.745) / (1 + e 0.953*loge (TG) + 0.139*BMI + 0.718*loge (GGT) + 0.053*WC—15.745) * 100 [16 (link)]. LAP was calculated with formula: LAP = (waist circumference (cm) – 58) × triglycerides (mmol/l) [18 (link)].
Yang B.L., Wu W.C., Fang K.C., Wang Y.C., Huo T.I., Huang Y.H., Yang H.I., Su C.W., Lin H.C., Lee F.Y., Wu J.C, & Lee S.D. (2015). External Validation of Fatty Liver Index for Identifying Ultrasonographic Fatty Liver in a Large-Scale Cross-Sectional Study in Taiwan. PLoS ONE, 10(3), e0120443.
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5

Hepatitis B Biomarker Quantification Protocol

Cited 1 time
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Blood collections were performed at study entry and follow-ups. Tests on serum markers were performed using commercial kits: HBsAg and HBeAg by radioimmunoassay (Abbott Laboratories, North Chicago, IL), ALT by serum chemistry autoanalyzer (model 736; Hitachi Co., Tokyo, Japan) using commercial reagents, serum HBV DNA levels by polymerase chain reaction (COBAS Amplicor; Roche Diagnostics, Indianapolis, IN) for baseline samples, and by real-time polymerase chain reaction (COBAS TaqMan; Roche Diagnostics, Indianapolis, IN) for follow-up samples. Serum HBsAg levels were quantified using the Elecsys HBsAg II Quant assay (Roche Diagnostics GmbH, Mannheim, Germany). M2BPGi was quantified with a novel sandwich immunoassay using the fully automated chemiluminescence enzyme immunoanalyzer, HISCL-5000 (Sysmex Co., Kobe, Japan)21 (link). Raw counts of M2BPGi were converted to a standardized cut-off index (COI) for analysis32 (link).
Liu J., Hu H.H., Lee M.H., Korenaga M., Jen C.L., Batrla-Utermann R., Lu S.N., Wang L.Y., Mizokami M., Chen C.J, & Yang H.I. (2017). Serum Levels of M2BPGi as Short-Term Predictors of Hepatocellular Carcinoma in Untreated Chronic Hepatitis B Patients. Scientific Reports, 7, 14352.
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6

Serum Biomarker Assays for Liver Disease

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Serum hepatitis B surface antigen and the HCV antibody were tested by radio-immunoassay (Abbott Laboratories, North Chicago, IL) and second-generation enzyme immunoassay (Abbott Laboratories, North Chicago, IL). Serum biochemistries were measured using a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany). The serum AFP level was tested using a radio-immunoassay kit (Serono Diagnostic SA, Coinsin/VD, Switzerland).
Kao W.Y., Chao Y., Chang C.C., Li C.P., Su C.W., Huo T.I., Huang Y.H., Chang Y.J., Lin H.C, & Wu J.C. (2015). Prognosis of Early-Stage Hepatocellular Carcinoma: The Clinical Implications of Substages of Barcelona Clinic Liver Cancer System Based on a Cohort of 1265 Patients. Medicine, 94(43), e1929.
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7

Hepatitis C and B Viral Serology

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Hepatitis C virus antibodies and hepatitis B surface antigen were examined at study entry. Hepatitis C virus antibodies were detected with a third-generation enzyme-linked immunosorbent assay (Abbott Laboratories, North Chicago, IL). Hepatitis B surface antigen was detected by radioimmunoassay (Abbott Laboratories).
Kawamura Y., Ikeda K., Arase Y., Sorin Y., Fukushima T., Kunimoto H., Hosaka T., Kobayashi M., Saitoh S., Sezaki H., Akuta N., Suzuki F., Suzuki Y, & Kumada H. (2015). New discriminant score to predict the fibrotic stage of non-alcoholic steatohepatitis in Japan. Hepatology International, 9(2), 269-277.
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8

Serum Biomarkers for Hepatitis Diagnosis

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Serum hepatitis B surface antigen (HBsAg) was tested using radioimmunoassay (Abbott Laboratories, North Chicago, IL). Antibody to hepatitis C virus (HCV) was measured by second-generation enzyme immunoassay (Abbott Laboratories, North Chicago, IL). Serum biochemistries, including albumin, bilirubin, alanine aminotransferase, were measured using a systemic multi-auto-analyzer (Technicon SMAC, Technicon Instruments Corp., Tarrytown, NY). Serum AFP level was measured by radioimmunoassay (Serono Diagnostic SA, Coinsin/VD, Switzerland).
Xie H., Wang H., An W., Ma W., Qi R., Yang B., Liu C., Gao Y., Xu B, & Wang W. (2014). The Efficacy of Radiofrequency Ablation Combined with Transcatheter Arterial Chemoembolization for Primary Hepatocellular Carcinoma in a Cohort of 487 Patients. PLoS ONE, 9(2), e89081.
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9

Serum Biochemistries and Liver Biomarkers

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Serum biochemistries were measured using a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany). The serum level of AFP was tested using a radio-immunoassay kit (Serono Diagnostic SA, Coinsin/VD, Switzerland). Serum levels of hepatitis B surface antigen (HBsAg) and hepatitis C virus antibody were tested by radio-immunoassay (Abbott Laboratories, North Chicago, IL) and second-generation enzyme immunoassay (Abbott). Previously described methods were used to calculate the ratio (AAR) of AST to ALT, APRI, and ALBI grade46 (link)–48 (link).
Lee P.C., Chiou Y.Y., Chiu N.C., Chen P.H., Liu C.A., Kao W.Y., Huo T.I., Huang Y.H., Hou M.C., Lin H.C., Wu J.C, & Su C.W. (2020). Liver stiffness measured by acoustic radiation force impulse elastography predicted prognoses of hepatocellular carcinoma after radiofrequency ablation. Scientific Reports, 10, 2006.
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10

Comprehensive Hepatitis Biomarker Profiling

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Serum hepatitis B surface antigen and HCV antibody were tested by radioimmunoassay (Abbott Laboratories, North Chicago, IL) and second-generation enzyme immunoassay (Abbott Laboratories, North Chicago, IL), respectively. Serum biochemistries, including albumin, bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (Alk-P), creatinine, glucose, and prothrombin time/international ratio (PT/INR) were measured using a Roche/Hitachi Modular Analytics System (Roche Diagnostics GmbH, Mannheim, Germany). The serum α-fetoprotein (AFP) level was tested using a radioimmunoassay kit (Serono Diagnostic SA, Coinsin, Switzerland).
Hung H.H., Chao Y., Chiou Y.Y., Li C.P., Lee R.C., Huo T.I., Huang Y.H., Chau G.Y., Su C.W., Yeh Y.C., Lin H.C., Lee S.D, & Wu J.C. (2014). A Comparison of Clinical Manifestations and Prognoses Between Patients with Hepatocellular Carcinoma and Child–Pugh Scores of 5 or 6. Medicine, 93(29), e348.
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