The laser beam (10W, 1064 nm) was tightly focused through a series of Keplerian beam expanders and a high numerical aperture objective (100 × 1.45, oil, Nikon). A high-resolution quadrant detector was used for position detection. To measure mechanical properties of the cytoplasm, 0.5-μm diameter latex particles (Sigma, L9654) were embedded in the gel and were endocytosed by the cells as they grew into cancer organoids. The linear region of the detector and the trap stiffness were calibrated with the same bead using an active power-spectrum method and equipartition theorem31 (link),51 (link),52 . The endocytosed bead was dragged at a constant velocity of 0.5 μm/s by the optical trap, and the force-displacement curve of the local cytoplasm was recorded. To calculate the apparent modulus (EA), the force and displacement were normalized by the cross-section area and the diameter of the particle respectively. The slope in the linear range of the normalized force-displacement curve was taken as the apparent modulus.
L9654
Manufactured by Merck Group
Sourced in United States
L9654 is a laboratory equipment product manufactured by Merck Group. It is a device designed for general laboratory use. The core function of L9654 is to facilitate standard laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
High numerical aperture objective, by Nikon (2 mentions)
L9904, by Merck Group (2 mentions)
L4655, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Trypticase soy agar, by Thermo Fisher Scientific (1 mentions)
Bromohexane, by Merck Group (1 mentions)
L5530, by Merck Group (1 mentions)
E coli 8739, by American Type Culture Collection (1 mentions)
L5155, by Merck Group (1 mentions)
Trypticase soy broth (tsb), by Thermo Fisher Scientific (1 mentions)
2 n morpholino ethanesulfonic acid, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Tert butanol, by Merck Group (1 mentions)
Lupasol wf, by BASF (1 mentions)
4 protocols using l9654
1
Measuring Cytoplasmic Mechanics in Cancer Organoids
2
Nanoplastic Capture Mechanisms in CNF Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescently labeled polystyrene (PS) particles (L9902, L9904, L4655, and L9654 from Sigma Aldrich) of different size (ø = 100 nm and ø = 1 µm) and surface charge (cationic and anionic) were used to analyze micro- and nanoplastic capturing ability, and to reveal the capturing mechanisms of CNF hydrogel and self-standing films. The nanoplastic particles are referred to as anionic (−) /cationic (+) PS(ø100 nm) and the microplastic particles as anionic (−)/cationic (+) PS(ø1µm).
Colloidal nanosized polystyrene (PS) particles (LB1 from Sigma Aldrich) were utilized for QCM-D experiments (ø = 100 nm, i.e. PS(ø100 nm)). These particles were utilized in a stabile form (i.e. utilized as provided by the supplier) or purified with the supplier’s protocol to remove most of the stabilizing agent. Stabile particles represent the native state of nanoplastics.
Surface charges for all polystyrene particles were determined by zeta potential measurements from 0.1 g L−1 dispersions (Zetasizer, Malvern) (Supplementary Table1 ).
Colloidal nanosized polystyrene (PS) particles (LB1 from Sigma Aldrich) were utilized for QCM-D experiments (ø = 100 nm, i.e. PS(ø100 nm)). These particles were utilized in a stabile form (i.e. utilized as provided by the supplier) or purified with the supplier’s protocol to remove most of the stabilizing agent. Stabile particles represent the native state of nanoplastics.
Surface charges for all polystyrene particles were determined by zeta potential measurements from 0.1 g L−1 dispersions (Zetasizer, Malvern) (Supplementary Table
3
Measuring Cytoplasmic Mechanics in Cancer Organoids
4
Antibacterial Surface Functionalization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hexafluoroisopropanol (HFIP) was purchased from Oakwood Chemicals, Estill, SC, USA. Tributylammonium chloride (TBAC), tert-butanol, potassium carbonate, bromohexane, iodomethane, Tween 20, N-3-dimethylaminopropyl-N-ethylcarbondiimide (EDC), N-hydroxy succinimide (NHS), 2-N-morpholino ethanesulfonic acid (MES), hexanes and fluorescent latex beads (L5155, L9904, L5530, L9654, L9529) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Trypticase soy broth, trypticase soy agar, phosphate buffered saline (PBS), and dey engley broth were purchased from Fisher, Waltham, MA, USA. E. coli (8739) and S. aureus (6538) were obtained from ATCC, Manassas, VA, USA. Vantocil 100 (B-1018-04) was purchased from Lonza, Allendale, NJ, USA. Lupasol WF (25,000 g/mol) was purchased from BASF, Florham Park, NJ, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!