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2 protocols using abs recognizing p53

1

Protein Expression Analysis Protocol

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Abs against phospho-ATM (Ser1981), ATM, phospho-Chk1 (Ser317), Chk1, phospho-Chk2 (Thr68), Chk2, phospho-p53 (Ser15), PTEN, phospho-CDK2 (Thr160), CDK2, Cyclin E, Cyclin B1, CDC2, phospho-CDC2 (Tyr15), cleaved PARP (Asp214), phospho-STAT3 (Ser727), STAT3, STAT5, caspase-3 and active caspase-3 (Asp175) were all obtained from Cell Signaling (Danvers, MA, USA). Abs recognizing p53 (EMD Millipore, Darmstadt, Germany), p21 (Santa Cruz, Dallas, Texas) and phospho-STAT5 (Ser726/731) (Sabbiotech, College Park, MD, USA) were obtained as indicated.
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2

Comprehensive Immunoblotting Analysis

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Abs against phospho-EGFR (Tyr1068), EGFR, GPX, phospho-p53 (Ser15), Cyclin B1, phospho-Histone H2A.X (Ser139), phospho-p38 MAPK (Thr180/Tyr182), p38 MAPK, iNOS, Arginase-1, TNF-α, IL-10, cleaved PARP (Asp214), PARP, caspase-3, active caspase-3 (Asp175), GPX and catalase were obtained from Cell Signaling (Danvers, MA). Abs recognizing p53 (EMD Millipore, Darmstadt, Germany), MnSOD (Enzo, Farmingdale, NY, USA), p21 (Santa Cruz, Dallas, Texas), p47phox (Ab-345) (SAB, College Park, MD, USA), phospho-p47phox (Ser345) (Elabscience, Houston, TX, USA), F4/80 and YY1 (GeneTex, Inc, Irvine, CA, USA), CD80 and CD163 (Abcam, Cambridge, MA, USA) were purchased as indicated. The SDS-PAGE and immunoblotting analysis was conducted as described previously [59 (link)].
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