For in vitro analysis, cells were removed from the culture in a 10 cm plate using a pipette. The harvested cells were incubated with FITC anti-HLA-ABC (1:100 or 1:500, #311404; BioLegend, San Diego, California, USA) or FITC anti-B2M (1:100 or 1:1000, # 316304; BioLegend) for human cancer cells (MIA PaCa-2 and KP-2), and FITC anti-H2 (1:50 or 1:100, #125508; BioLegend) for mouse cancer cells (KPC-1, KPC-2, and KPC-3). FITC IgG (#400506; BioLegend) or FITC IgG (#400108; BioLegend) was used as the control. The cells were then stained for 30 min at 4 °C. Following the removal of antibodies using phosphate-buffered saline, the cells were stained with propidium iodide (PI) (1:1000, #421301; BioLegend). Subsequently, the stained cells were analyzed using FACSVerse (BD Biosciences). The gating mechanisms used for the FCM analyses are shown in Supplementary Figure S3 E. The acquired data were analyzed using FlowJo 10.5.3.
Fitc igg
Manufactured by BioLegend
Sourced in United States
FITC IgG is a fluorochrome-conjugated immunoglobulin G (IgG) antibody. It is designed for use as a tool in flow cytometry, immunohistochemistry, and other immunoassays.
Automatically generated - may contain errors
3 protocols using fitc igg
1
Flow Cytometry Analysis of Cell Surface Markers
2
Flow Cytometry Analysis of Stem Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treatment, the cells were digested and washed twice with cold PBS containing 2% BSA, then resuspended in 100 μL PBS and incubated with the antibody FITC-IgG, PE-IgG and FITC-CD44, PE-CD24 (BioLegend, San Diego, CA, USA) for 30 min at 4 °C in the dark. The expressions of stemness-related markers of CD24 and CD44 were detected by flow cytometry, and the ratio of CD44+ and CD24− cells was analyzed.
3
Phenotypic Analysis of Fetoplacental Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!