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Human il 17f duoset

Manufactured by R&D Systems
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The Human IL-17F Duoset is a laboratory reagent kit designed to quantitatively measure the concentration of human interleukin-17F in biological samples. The kit includes the necessary components to perform a sandwich enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

96 well tissue culture plate, by BD (1 mentions) Human cxcl8 il 8 duoset, by R&D Systems (1 mentions) Human th cytokine panel 13 plex, by BioLegend (1 mentions) Human ccl2 duoset, by R&D Systems (1 mentions)

2 protocols using human il 17f duoset

1

Cytokine Secretion in Cell Cultures

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2,500 cells were plated on each well of a 96-well tissue culture plate (BD) with at least 10 replicates per condition. The medium was left unchanged starting from 48 h after plating or 16 h after transfection. After 48 h, the medium was collected and CXCL9, CXCL11, CXCL8, IL-17F, and TSLP levels were determined using mouse CXCL9/MIG Duoset, human CXCL11/I-TAC Duoset, human CXCL8/IL-8 Duoset, human IL-17F Duoset, and human TSLP Duoset (all from R&D Systems) according to the manufacturer’s protocol. Every experiment was performed at least three times, each with at least three biological replicate samples.
Chung B.M., Arutyunov A., Ilagan E., Yao N., Wills-Karp M, & Coulombe P.A. (2015). Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. The Journal of Cell Biology, 208(5), 613-627.
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2

Quantification of Cytokine Secretion

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ELISAs were carried out on supernatants from cultured cells (frozen at -20°C) using: IL-17A Human ELISA Kit (eBioscience), Human IL-17F DuoSet (R&D), Human CXCL8 DuoSet (R&D), Human CCL2 DuoSet (R&D), IL-6 Assay Kit (Cisbio) and Human Th Cytokine Panel (13-plex) (BioLegend), as per the manufacturers’ instructions.
Cole S., Murray J., Simpson C., Okoye R., Tyson K., Griffiths M., Baeten D., Shaw S, & Maroof A. (2020). Interleukin (IL)-12 and IL-18 Synergize to Promote MAIT Cell IL-17A and IL-17F Production Independently of IL-23 Signaling. Frontiers in Immunology, 11, 585134.
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