Primary antibodies against Snail1 (cat. no. 125918) were purchased from GeneTex, Inc. (Irvine, CA, USA). Primary antibodies against Wnt1 (ab85060), Wnt8a (ab130930), phosphorylated glycogen synthase kinase 3β (P-GSK3β; ab130937) and GAPDH (ab37168) were obtained from Abcam (Cambridge, UK). Primary antibodies against Wnt3a (bs1700R) and Wnt5a (bs1948R) were purchased from BIOSS (Beijing, China). The primary antibody against Wnt11 (sc50360) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Antibodies against β-catenin (BA0426) and β-actin (BM0627) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China). The antibody against GSK3β (22104-1-AP) was purchased from Wuhan Sanying Biotechnology (Wuhan, China). The bicinchoninic acid (BCA) protein assay kit was purchased from Beyotime Institute of Biotechnology (Haimen, China). TRIzol (252250AX) was obtained from Aidlab Biotechnologies Co., Ltd. (Beijing, China). M-MLV Reverse Transcriptase (RNase H; CO2010A) and ddH2O (DNase/RNase Free; C1D230A) were purchased from GeneCopoeia, Inc. (Rockville, MD, USA) and RNase Inhibitor (I21222) was obtained from TransGen Biotech, Inc. (Beijing, China).
Sc50360
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc50360 is a laboratory instrument designed for sample preparation and analysis. It is a centrifuge that can be used to separate various biological components based on their density or sedimentation rate. The core function of Sc50360 is to provide efficient and consistent sample separation for downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab85060, by Abcam (1 mentions)
Ab37168, by Abcam (1 mentions)
Bs 1700r, by Bioss Antibodies (1 mentions)
Bs1948r, by Bioss Antibodies (1 mentions)
Bm0627, by Wuhan Boster Biological Technology (1 mentions)
Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions)
Mab645, by R&D Systems (1 mentions)
Mab5005, by R&D Systems (1 mentions)
Tgx stain free fastcast acrylamide kit, by Bio-Rad (1 mentions)
2 protocols using sc50360
1
Western Blot Analysis of Wnt Pathway Proteins
2
Immunodetection of Wnt Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The immunocomplexes bound to the protein G sepharose beads obtained from CM-CITED2 and CM-Ctl as described above, were washed 5 times with PBS1× and immunopurified proteins were analyzed by western blot as previously described27 (link), with a rat monoclonal anti-WNT5A (MAB645, R&D Systems) or rabbit polyclonal anti-WNT11 (SC-50360, Santa Cruz) antibodies used at 1:1000 and 1:200 dilution, respectively. Loading was monitored by staining of PVDF membrane-bound proteins by ponceau s. Western blotting assays performed using 15 μg of protein extracts from zebrafish embryos harvested at 24 and 48 hpf after microinjection of either combined AUG and SPLICING (2.5 ng of each morpholino) or non-injected the control embryos (Control) prepared using conditions previously described27 (link). Proteins were separated by SDS-PAGE on TGX Stain-Free™ FastCast™ Acrylamide Kit (Bio-Rad). CITED2 protein was detected in zebrafish extracts using an anti-CITED2 rat monoclonal IgG2A antibody (MAB5005, R&D System) used at 1:500 dilution as recommended by the manufacturer. Loading control was monitored by detection of total stained proteins transferred to the PVDF membrane.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!