Anti otub1

The c-Maf and Otub1 plasmids were subcloned into a pcDNA3.1 vector carrying an HA, or Myc tag. The luciferase reporter driven by c-Maf recognition element (MARE) (5′-TGCGAGTGAGGCA-3′) (pGL4-MARE.Luci) was synthesized by GeneWiz, Inc. (Suzhou, Jiangsu, China) [7 (link)]. The Otub1-siRNA and control-siRNA were purchased from Ribobio Inc. (Guangzhou, China) [11 ].
The antibodies used for Western blot were as follows: anti-Otub1 and anti-Ub were from Santa Cruz Biotechnology Co. Ltd (Santa Cruz, CA); anti-glyceraldehyde-3-phosphatedehydrogenase (GAPDH), anti-integrin beta 7 (ITGB7) and anti-c-Maf were from Proteintech Group, Inc. (Wuhan, China); anti-HA and anti-Myc were obtained from MBL Biotech Co., (Beijing, China); anti-poly(ADP-ribose) polymerase (PARP), anti-Caspase 3 and anti-cyclin D2 (CCND2) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies of Otud4, Otud5, and Otud7b were provided by Beyotime Institute of Biotechnology (Haimen, China).

Sigma Chemical Co. provided honokiol, cycloheximide and MG132 (St. Louis, MO, USA), and R&D system supplied recombinant human recombinant TRAIL and z-VAD-fmk (Minneapolis, MN, USA). Enzo Life Sciences provided lactacystin (Ann Arbor, MI, USA). The primary antibodies were as follows: Cell Signaling Technology supplied anti-PARP, anti-cleaved caspase-3, anti-Bcl-xL, anti-DR5, anti-CHOP, and anti-UCHL1 (Beverly, MA, USA). Sigma Chemical Co. supplied anti-actin (St. Louis, MO, USA). Enzo Life Sciences provided anti-pro-caspase-3 and anti-c-FLIP (San Diego, CA, USA). BD Biosciences provided anti-Bim and anti-XIAP (San Jose, CA, USA). Abcam supplied anti-DR4 (Cambridge, MA, USA). R&D system supplied anti-survivin (Minneapolis, MN, USA). Santa Cruz Biotechnology provided anti-Mcl-1, anti-Bcl-2, anti-cIAP2, anti-ATF4, anti-Ub, anti-Cbl, anti-Itch, anti-USP14, anti-USP33, anti-OTUB1, anti-TRABID, and anti-STAMBPL1 (St. Louis, MO, USA). Bethyl Laboratories Inc provided anti-USP7 and anti-USP8 (Montgomery, TX, USA). Novus Biologicals supplied anti-USP53 (Centennial, CO, USA). Abnova provided anti-USP9X (Taipei City, Taiwan). The siRNAs were as follows: GFP (control) siRNA (Bioneer, Daejeon, Korea), DR5 siRNA (Invitrogen, Carlsbad, CA, USA), and STAMBPL1 siRNA (Santa Cruz Biotechnology, St. Louis, MO, USA). STAMBPL1 plasmid was a gift from Dr. H.C. Kang (Ajou University, Suwon, Korea).