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Snu 620 cells

Manufactured by Korean Cell Line Bank

SNU-620 cells are a human cell line derived from a gastric adenocarcinoma. The cells exhibit an epithelial-like morphology and are routinely cultured in vitro. The SNU-620 cell line is a valuable research tool for studies related to gastric cancer biology and drug development.

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2 protocols using snu 620 cells

1

Culturing Human Gastric Cancer Cell Lines

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Human gastric cancer SNU-16 cells (No. 00016), SNU-620 cells (No. 00620), and SNU-484 cells (No. 00484) were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea). These cells were maintained in RPMI-1640 medium (GenDEPOT, Barker, TX) supplemented with 10% fetal bovine serum (Young In Frontier, Seoul, Korea) and 1% penicillin-streptomycin (GenDEPOT). The cells were cultured in a 5% CO2 humidified incubator at 37°C.
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2

Establishment of 5-FU-resistant Gastric Cancer Cells

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Human gastric carcinoma SNU-620 cells were purchased from Korean Cell Line Bank (Seoul, Korea). Cells were grown in RPMI-1640 media supplemented with 10% (v/v) FBS, penicillin (100 U/ml)/streptomycin (100 µg/ml) at 37°C in a humidified CO2 (5%)-controlled incubator. 5-FU-resistant SNU-620/5-FUR cells were established by repeated cultures of SNU-620 with constant treatment with 7.5 µM 5-FU.
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