Cells washed with phosphate-buffered saline (PBS) were lysed in SDS buffer and boiled at 94 °C for 5 min. After measuring protein quantity by Bradford, equal amounts of protein were resolved by SDS-PAGE, transferred to a nitrocellulose membrane (Millipore) and probed with one of the following antibodies: mouse anti-Chk1 (1:1000, abcam), rabbit anti-p21 (1:500, Cell signaling), rabbit anti- p53 (1:1000, Cell Signaling), rabbit anti-SETD8 (1:1000, Cell Signaling), mouse anti-β-actin (1:20,000, Sigma), rabbit anti-H2A.X and anti-phospho-H2A.X-Ser139 (1:1000, Cell signaling), rabbit anti-H4-K20me1 (1:1000 Cell Signaling), and rabbit anti-Histone H4 (1:1000, Cell Signaling), rabbit anti-tubulin (1:1000, Cell signaling). Membranes were then incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies. The immunoreactive bands were detected by chemiluminescence (Pierce).
Mouse anti chk1
Manufactured by Abcam
Mouse anti-Chk1 is a primary antibody that recognizes the Chk1 protein, a key regulator of the cell cycle checkpoint in response to DNA damage. This antibody is suitable for use in various immunoassays, such as Western blotting, to detect and study the expression of Chk1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Merck Group (2 mentions)
Nitrocellulose membrane, by Merck Group (1 mentions)
Rabbit anti p21, by Cell Signaling Technology (1 mentions)
Rabbit anti tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti setd8, by Cell Signaling Technology (1 mentions)
Rabbit anti p53, by Cell Signaling Technology (1 mentions)
Rabbit anti lc3b antibody, by Merck Group (1 mentions)
Irdye secondary antibody, by LI COR (1 mentions)
2 protocols using mouse anti chk1
1
Western Blot Analysis of Key Proteins
2
Protein Quantification via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blot analysis was performed
on total protein extracts (100 μg/lane) using the following
primary antibodies: a mouse anti-Chk1 (Abcam 1:500), a rabbit anti-Chk1
(phospho Ser317) (Abcam 1:100), a rabbit anti-LC3B antibody (Sigma-Aldrich
1:1000), and a mouse anti-β-actin (Sigma, 1:100). We used IRDye
secondary antibodies (LI-COR, 1:30,000) for band imaging with the
Odyssey Imager.
on total protein extracts (100 μg/lane) using the following
primary antibodies: a mouse anti-Chk1 (Abcam 1:500), a rabbit anti-Chk1
(phospho Ser317) (Abcam 1:100), a rabbit anti-LC3B antibody (Sigma-Aldrich
1:1000), and a mouse anti-β-actin (Sigma, 1:100). We used IRDye
secondary antibodies (LI-COR, 1:30,000) for band imaging with the
Odyssey Imager.
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