fMRI data sensitive to blood oxygenation level-dependent (BOLD) contrast were collected with a Discovery MR750 3 Tesla scanner (GE Healthcare, Milwaukee, Wisconsin) equipped with a 32-channel head-coil. Randomized fast-event related T2*-weighted echo planar imaging (EPI) scans [TR=2000 ms, TE=32 ms, FOV=230 mm2, 64x64 matrix, 40 2.6mm axial slices with 1.4 mm gap, flip angle=90°] were obtained while subjects performed the WTP task. A high-resolution T1-weighted image [spoiled gradient recalled (SPGR), TR=8 ms, TE=3 ms, FOV=25 cm, ~1 mm3 voxels] was also obtained for anatomical reference. Resting-state data were acquired in the sagittal plane using a multi-echo simultaneous multi-slice (MESMS) data acquisition (Olafsson et al., 2012 ), where three slices are simultaneously excited for each of the three echoes collected to control for head motion (FOV 200mm2; matrix: 100x40; no gap between slices; TR=2000ms; TE1=15.5ms, TE2=36.7ms, TE3=57.9ms, flip angle=30; in-plane sensitivity encoded (SENSE) acceleration factor of 2.5). During the resting state scans subjects were instructed to focus on a visually presented fixation cross displayed on a black background and were told: “Try not to think about anything in particular.” Resting state data were acquired before subjects performed the WTP task.
Discovery mr750 3 tesla scanner
Manufactured by GE Healthcare
Sourced in United States
The Discovery MR750 3 Tesla scanner is a magnetic resonance imaging (MRI) system developed by GE Healthcare. It operates at a magnetic field strength of 3 Tesla, which provides high-quality images for clinical diagnosis and research applications. The core function of the Discovery MR750 is to generate detailed anatomical and functional images of the human body using powerful magnetic fields and radio waves.
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4 protocols using discovery mr750 3 tesla scanner
1
fMRI Acquisition and Resting-State Analysis
2
3T fMRI Protocol for Visual Stimulus
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The imaging data for all four experiments were collected on a General Electric (GE) Discovery MR750 3 Tesla scanner (General Electric Medical Systems, Milwaukee, USA) using a 32-channel receive-only phased-array head coil in the Mind Research Imaging center at the National Cheng Kung University. High-resolution structural images were acquired with fast-SPGR consisting of 166 axial slices (TR/TE/flip angle 7.6 ms/3.3 ms/12°; a field of view (FOV) 22.4 × 22.4 cm2; matrices 224 × 224; slice thickness 1 mm). The functional EPI images were collected using an interleaved T2* weighted gradient-echo planar imaging (EPI) pulse sequence (TR/TE/flip angle, 2000 ms/30 ms/77°; matrices, 64 × 64; FOV, 22 × 22 cm2; slice thickness, 4 mm; voxel size, 3.4375 × 3.4375 × 4 mm). A total of 75 volumes were acquired for each block; the first five were dummy scans and were discarded to avoid T1 equilibrium effects. Moreover, localizer scans (5 min) and distortion correction scans (B0 field map and a pair of spin-echo EPI scans with opposite phase-encoded directions) are also acquired. The visual stimulus was displayed using Presentation (Version 18.0, Neurobehavioral Systems, Inc., Berkeley, CA, www.neurobs.com ) on an MSI GS65 Stealth laptop and was projected onto a screen that was viewed through a mirror attached to the head coil.
3
Resting-State fMRI Acquisition Protocol
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Imaging was performed using a GE DISCOVERY MR750 3-Tesla scanner with an 8-channel head coil. Resting-state functional MR images were acquired using an axial plane, gradient-echo echo planar imaging sequence with TR = 2200 ms, TE = 26 ms, flip angle = 79°, field of view = 256 x 256 mm2, matrix size of 64 × 64 mm2, voxel size = 4 x 4 x 4 mm3, and slice spacing = 4 mm. 274 consecutive volumes were collected over a 10 min acquisition period, during which subjects were instructed to remain still, keep their eyes closed, and not to think of anything specifically. In addition, a 3D Sagittal T1 SPGR was acquired for anatomical reference (TE = 1.9 ms, TI = 400 ms, flip angle = 11°, voxel size = 0.93 × 0.93 × 1.2 mm3).
4
Structural MRI of Brain Lesions
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Structural magnetic resonance images of each participant were obtained at the VU Medical Center, Amsterdam. T1-weighted volumes were acquired with a Discovery MR750 3 Tesla scanner (GE, Waukesha, WI, USA) running a 3D fast spoiled gradientrecalled-echo sequence. The volume consisted of 172 sagittal slices (256 Â 256). The scans were reviewed by a certified radiologist, where after lesions were rated as cortical, subcortical or corticalsubcortical, in line with the Automated Anatomical Labelling atlas. Furthermore, the clinically obtained information on the side of the affected hemisphere (left/right) was checked based on the MRI data, which did not show discrepancies.
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