Gapdh sirna

Manufactured by Horizon Discovery

GAPDH siRNA is a laboratory reagent designed for gene silencing experiments. It targets the GAPDH gene, which encodes the glyceraldehyde 3-phosphate dehydrogenase enzyme, a key protein involved in cellular metabolism. The siRNA sequence is optimized to efficiently knock down GAPDH expression in a variety of cell lines.

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Lab products found in correlation

Dharmafect 1 reagent, by Horizon Discovery (1 mentions) Control sirna, by Horizon Discovery (1 mentions) On targetplus non targeting control pool, by Horizon Discovery (1 mentions)

Spelling variants of this product

SiRNAs (161 citations) GAPDH (6 citations) Non-targeting or GAPDH-targeting siRNA (2 citations)

3 protocols using gapdh sirna

GAPDH Knockdown using siRNA

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GAPDH siRNA (On-target plus smart pool) and Control siRNA (On-target plus siControl non-targeting pool) were purchased from Dharmacon and were transfected according to the manufacturer's guidelines using DharmaFECT I reagent.

Duan L., Perez R.E., Davaadelger B., Dedkova E.N., Blatter L.A, & Maki C.G. (2015). p53-regulated autophagy is controlled by glycolysis and determines cell fate. Oncotarget, 6(27), 23135-23156.

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Mammalian Plasmids for Soluble Guanylate Cyclase

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pCMV5 mammalian expression plasmids containing rat sGCα(1–690), sGCβ(1–619), or sGCβ(1–619) Y135A R139A with a tetracysteine motif (CCPGCC) at residues 239 to 244 have been reported previously (19 (link)). pcDNA3.1 plasmids encoding HA-tagged human GAPDH wild type or H53A that are resistant to GAPDH siRNA (Dharmacon) have been reported previously (20 (link)).

Dai Y., Faul E.M., Ghosh A, & Stuehr D.J. (2022). NO rapidly mobilizes cellular heme to trigger assembly of its own receptor. Proceedings of the National Academy of Sciences of the United States of America, 119(4), e2115774119.

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Silencing TRPM2 in Human VSMCs

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Human VSMCs were transiently transfected with 100 nmol/L with ON‐TARGET plus Human TRPM2 (7226) small interfering RNA (siRNA)—SMARTpool from Dharmacon (L‐004193‐00‐0050) for 7 hours. For housekeeping, GAPDH siRNA was used (D‐001830‐10‐20 ‐ Dharmacon). As a control, we used ON‐TARGETplus Non‐targeting Control Pool from Dharmacon (D‐001810–10‐20). Experiments were performed at 48 hours after transfections. Transfection efficiency was measured by real‐time western blotting.

Neves K.B., Alves‐Lopes R., Montezano A.C, & Touyz R.M. (2023). Role of PARP and TRPM2 in VEGF Inhibitor‐Induced Vascular Dysfunction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 12(4), e027769.

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