Acetobromo α d glucuronic acid methyl ester

Manufactured by Merck Group

Sourced in Germany, United States

Acetobromo-α-d-glucuronic acid methyl ester is a chemical compound used in laboratory settings. It serves as a reagent for various organic synthesis procedures. The compound's core function is to facilitate specific chemical reactions, however, a detailed description of its intended use cannot be provided in an unbiased and factual manner without extrapolation.

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Lab products found in correlation

Alamethicin, by Santa Cruz Biotechnology (1 mentions) Glutamine, by Merck Group (1 mentions) 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions) Uridine 5 diphosphoglucuronic acid trisodium salt, by Merck Group (1 mentions) Mouse il 1βelisa, by BioLegend (1 mentions) Mgcl2, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Griess reagent, by Merck Group (1 mentions) Selenium, by Thermo Fisher Scientific (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Transferrin, by Thermo Fisher Scientific (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) K2co3, by Merck Group (1 mentions) Purelab ultra system, by Elga LabWater (1 mentions) Pyridine, by Merck Group (1 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions)

Spelling variants of this product

Glucuronic acid (86 citations) D-glucuronic acid (65 citations) Acetobromo-glucuronic acid methyl-ester (2 citations)

2 protocols using acetobromo α d glucuronic acid methyl ester

Glucuronic Acid Metabolism Assay

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ORV and GN
were purchased from
TCI Chemicals (Zwijndrecht, Belgium). Acetobromo-α-d-glucuronic acid methyl ester, uridine 5′-diphosphoglucuronic
acid trisodium salt (UDPGA), MgCl₂, Tris–HCl, LPS,
Roswell Park Memorial Institute (RPMI) medium, Dulbecco’s modified
Eagle’s medium (DMEM), fetal bovine serum (FBS), Griess reagent,
2′,7′-dichlorodihydrofluorescein diacetate acetyl (DCFH₂-DA), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), trifluoroacetic acid (TFA), dimethyl
sulfoxide (DMSO), and glutamine were obtained by Sigma-Aldrich (Steinheim,
Germany). Alamethicin was purchased from Santa Cruz Biotechnology
(Heidelberg, Germany). Male rat and human hepatic S9 fractions were
obtained by Biopredic (Saint Grégoire, France). RAW 264.7 cells
were provided by American Type Culture Collection (ATCC, Manassas,
VA, U.S.A.). Mouse TNF-α enzyme-linked immunosorbent assay (ELISA)
was purchased by InmunoTools (Friesoythe, Germany), and mouse IL-1β
ELISA was provided by BioLegend (San Diego, CA, U.S.A.).

Hornedo-Ortega R., Jourdes M., Da Costa G., Courtois A., Gabaston J., Teissedre P.L., Richard T, & Krisa S. (2022). Oxyresveratrol and Gnetol Glucuronide Metabolites: Chemical Production, Structural Identification, Metabolism by Human and Rat Liver Fractions, and In Vitro Anti-inflammatory Properties. Journal of Agricultural and Food Chemistry, 70(41), 13082-13092.

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Cell Culture and Hemisynthesis Protocol

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For cell culture, Dulbecco modified Eagles minimal essential medium (DMEM)/HAM's F12 (F-12 Nutrient Medium) Glutamax, insulin, transferrin and selenium were obtained from Thermo Fisher (Waltham, MA, USA). Foetal bovine serum (FBS) was purchased from Corning (New York, NY, USA). Antibiotics (streptomycin and penicillin) and trypsin/EDTA were obtained from Lonza (Basel, Switzerland). The PA was purchased from Sigma-Aldrich (St Louis, MO, USA).
Concerning hemisynthesis and UPLC-DAD-MS analysis, acetobromo-α-Dglucuronic acid methyl-ester, pyridine and K 2 CO 3 were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France); and ultra-pure methanol, ethanol, acetonitrile, ethyl acetate and formic acid were purchased from Fisher Scientific. Milli-Q water used for these experiments was produced in the laboratory using Purelab Ultra System (Elga Lab Water, High Wycombe, UK).

Beaumont P., Amintas S., Krisa S., Courtois A., Richard T., Eseberri I, & Portillo M.P. (2024). Glucuronide metabolites of trans-ε-viniferin decrease triglycerides accumulation in an in vitro model of hepatic steatosis. Journal of physiology and biochemistry.

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