Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA). Gold nanoparticles (15 nm) were purchased from BBI solutions. Sodium citrate and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonate (HEPES) were purchased from Mandel Scientific (Guelph, ON). Sodium silicate and cardiotoxin (C9759) were purchased from Sigma Aldrich (St. Louis, MO). Phusion High-fidelity DNA Polymerase was purchased from NEB (Ipswich, MA). The MEGAscript T7 kit, the MEGAclear kit, the PageBlue solution, the propidium iodide, and the PureLink genomic DNA kit were purchased from Thermo Fischer (Waltham, MA). Mini-PROTEAN TGX Gels (4–20%) were purchased from Bio-Rad (Hercules, CA). MTeSR-1 media gentle cell dissociation reagent was purchased from StemCell Technologies (Vancouver, Canada). Matrigel was purchased from BD Biosciences (San Jose, CA). DMEM media, non-essential amino acids, penicillin-streptomycin, DPBS and 0.05% trypsin were purchased from Life Technologies (Carlsbad, CA). EMD Millipore Amicon Ultra-4 100kDa was purchased from Millipore (Germany).
Emd millipore amicon ultra 4 100kda
Manufactured by Merck Group
Sourced in Germany
The EMD Millipore Amicon Ultra-4 100kDa is a centrifugal filter unit designed for concentrating and purifying macromolecules such as proteins, peptides, and nucleic acids. It is capable of retaining molecules with a molecular weight above 100 kilodaltons.
Automatically generated - may contain errors
Lab products found in correlation
Sodium silicate, by Merck Group (3 mentions)
Purelink genomic dna kit, by Thermo Fisher Scientific (3 mentions)
Pageblue solution, by Thermo Fisher Scientific (3 mentions)
Megaclear kit, by Thermo Fisher Scientific (3 mentions)
Trypsin, by Thermo Fisher Scientific (3 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (3 mentions)
Mini protean tgx gels 4 20, by Bio-Rad (3 mentions)
Megascript t7 kit, by Thermo Fisher Scientific (3 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Phusion high fidelity dna polymerase, by New England Biolabs (3 mentions)
Dmem media, by Thermo Fisher Scientific (3 mentions)
Matrigel, by BD (3 mentions)
Propidium iodide, by Thermo Fisher Scientific (3 mentions)
Cardiotoxin c9759, by Merck Group (2 mentions)
Mtesr 1 media gentle cell dissociation reagent, by STEMCELL (2 mentions)
Oligonucleotide, by Integrated DNA Technologies (2 mentions)
3 protocols using emd millipore amicon ultra 4 100kda
1
Synthesis and Characterization of Nanoparticles
2
Synthesis and Characterization of Nanoparticles
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Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA). Gold nanoparticles (15 nm) were purchased from BBI solutions. Sodium citrate and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonate (HEPES) were purchased from Mandel Scientific (Guelph, ON). Sodium silicate and cardiotoxin (C9759) were purchased from Sigma Aldrich (St. Louis, MO). Phusion High-fidelity DNA Polymerase was purchased from NEB (Ipswich, MA). The MEGAscript T7 kit, the MEGAclear kit, the PageBlue solution, the propidium iodide, and the PureLink genomic DNA kit were purchased from Thermo Fischer (Waltham, MA). Mini-PROTEAN TGX Gels (4–20%) were purchased from Bio-Rad (Hercules, CA). MTeSR-1 media gentle cell dissociation reagent was purchased from StemCell Technologies (Vancouver, Canada). Matrigel was purchased from BD Biosciences (San Jose, CA). DMEM media, non-essential amino acids, penicillin-streptomycin, DPBS and 0.05% trypsin were purchased from Life Technologies (Carlsbad, CA). EMD Millipore Amicon Ultra-4 100kDa was purchased from Millipore (Germany).
3
CRISPR Reagent Preparation Protocol
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Unmodified crRNA, 5’ Amine-crRNA, 5’ Azide-crRNA, 5’ Thiol-crRNA, 3’ Amine-crRNA, 5’ Amine-Donor, 3’ Amine-Donor, 5’ Azide-Donor, and various DNA sequences were purchased from Integrated DNA Technology (IDT). Phusion High-fidelity DNA Polymerase was purchased from NEB (Ipswich, MA). The Megascript T7 kit, the Megaclear kit, the PageBlue solution, the propidium iodide, and the PureLink genomic DNA kit were purchased from Thermo Fisher (Waltham, MA). Mini-PROTEAN TGX Gels (4–20%) were purchased from Bio-Rad (Hercules, CA). 4-(2-hydroxyethyl) piperazine-1-ethanesulfonate (HEPES) were purchased from Mandel Scientific (Guelph, ON). Sodium silicate was purchased from Sigma Aldrich (St. Louis, MO). Matrigel was purchased from BD Biosciences (San Jose, CA). DMEM media, non-essential amino acids, penicillin-streptomycin, DPBS and 0.05% trypsin were purchased from Life Technologies (Carlsbad, CA). EMD Millipore Amicon Ultra-4 100 kDa and 300 kDa were purchased from Millipore (Germany). Streptococcus pyogenes Cas9 (spCas9) and Acidaminococcus sp. Cpf1 (AsCpf1) were purchased from the QB3 Macrolab from UC Berkeley. PAsp(DET) polymer was a generous gift from Dr. Kataoka’s group (Miyata et al., 2008 (link); Kim et al., 2010 (link), 2014 (link)).
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