Xylene

Manufactured by Vetec

Sourced in Brazil

Xylene is a clear, colorless, flammable liquid hydrocarbon. It is commonly used as a solvent in various laboratory applications.

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Lab products found in correlation

2 protocols using xylene

Histological Analysis of Thyroid Tissue

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The thyroids were collected and fixed in 10% formaldehyde (pH 7.4) for 48 h, followed by dehydration in ethanol (Vetec, Rio de Janeiro, Brazil) and clarification in xylene (Vetec, Rio de Janeiro, Brazil). Tissues were embedded in paraffin and cut at the thickness of 5 μm. Sections were stained with hematoxylin and eosin for topographic analysis according to Andrade et al. (27 (link)). High-resolution images (1600 × 1200 pixels) were obtained using digital camera (QImaging Retigar-2000R Fast 1394 Mono, Surrey BC, Canada) coupled to light microscope (Olympus BX50). High-quality images were captured with the QCapture Pro7 (QImaging).

da Silva M.M., Xavier L.L., Gonçalves C.F., Santos-Silva A.P., Paiva-Melo F.D., de Freitas M.L., Fortunato R.S., Miranda-Alves L, & Ferreira A.C. (2018). Bisphenol A increases hydrogen peroxide generation by thyrocytes both in vivo and in vitro. Endocrine Connections, 7(11), 1196-1207.

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Antioxidant and Anticholinesterase Assays

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Bovine serum albumin, gallic acid, rutin and quercetin, aluminium chloride, acetylcholine, 5,5′-dithiobis(2-nitrobenzoic acid) (DNTB), 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide (BW284c51), tetraisopropyl pyrophosphoramide (iso-OMPA), acetylthiocholine iodide, S-butyrylthiocholine iodide, epinephrine bitartrate, and eosin were purchased from Sigma.
This work also made use of hydrogen peroxide (CRQ), trichloroacetic acid (TCA) (Vetec), thiobarbituric acid (TBA) (TediaBrasil), n-butanol (Synth), glycine (Vetec), NaH₂PO₄H₂O (Cromoline), Na₂HPO₄ (Synth), coomassie brilliant blue (Amresco), ethyl alcohol (Synth), xylene (Vetec), Paraplast (Merck), and hematoxylin (Merck).
All electrolyte salts, solvents and reagents were of analytical grade. Electrolyte solutions were prepared with double distilled Milli-Q water (conductivity ≤ 0.1 μS·cm⁻¹) (Millipore S. A., Molsheim, France).

de Oliveira T.S., Thomaz D.V., da Silva Neri H.F., Cerqueira L.B., Garcia L.F., Gil H.P., Pontarolo R., Campos F.R., Costa E.A., dos Santos F.C., de Souza Gil E, & Ghedini P.C. (2018). Neuroprotective Effect of Caryocar brasiliense Camb. Leaves Is Associated with Anticholinesterase and Antioxidant Properties. Oxidative Medicine and Cellular Longevity, 2018, 9842908.

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