Goat anti mouse igg or iga conjugated with horseradish peroxidase

Manufactured by Southern Biotech

Goat anti-mouse IgG or IgA conjugated with horseradish peroxidase is a reagent used in various immunoassay techniques. It serves as a secondary antibody, binding to mouse immunoglobulin (IgG or IgA) and enabling the detection of target analytes through the enzymatic activity of the conjugated horseradish peroxidase.

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Lab products found in correlation

Microplate reader, by Agilent Technologies (1 mentions) Tmb peroxidase substrate, by Southern Biotech (1 mentions)

Close spelling variants of this product

Horseradish peroxidase‐conjugated anti‐mouse IgA (2 citations) Goat anti-mouse IgA (26 citations) Anti-mouse IgA (14 citations) Horseradish peroxidase (23 citations)

2 protocols using goat anti mouse igg or iga conjugated with horseradish peroxidase

Quantifying PspA-specific Antibody Levels

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PspA-specific antibody production was determined by ELISA. Accordingly, 96-well immunoplates were coated with PspA (0.05 μg/well) at 4°C overnight. The immunoplates were treated with 1% BSA in PBS for 2 h at room temperature to prevent nonspecific binding. After the plates were washed with 0.05% Tween in PBS, 2-fold serial dilutions of samples were added to wells, and the plates were incubated at 4°C overnight. After the plates were washed with 0.05% Tween in PBS, goat anti-mouse IgG or IgA conjugated with horseradish peroxidase (SouthernBiotech, Birmingham, AL) was added to the immunoplates and incubated for 1 h at room temperature. After the plates were washed with 0.05% Tween in PBS, PspA-specific antibodies were detected by using TMB peroxidase substrate and reading the absorbance at 450 nm.

Suzuki H., Watari A., Hashimoto E., Yonemitsu M., Kiyono H., Yagi K., Kondoh M, & Kunisawa J. (2015). C-Terminal Clostridium perfringens Enterotoxin-Mediated Antigen Delivery for Nasal Pneumococcal Vaccine. PLoS ONE, 10(5), e0126352.

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ELISA Quantification of Antibodies

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ELISA plates were coated with 2 μg/ml of K. pneumoniae antigen at 4°C, overnight (The antigen preparation is mentioned in the supplemental file). Coated plates were rinsed with washing buffer (0.05% Tween 20 in PBS), incubated for 2 hours with blocking buffer (1% BSA and 0.1% Tween 20 in PBS), and washed before the addition of serially diluted serum, bronchial alveolar lavage (BAL), or fecal supernatants. After 2 hours of incubation at room temperature and plate washing, bacterial specific antibodies were detected with 1/5000 goat anti-mouse IgG or IgA conjugated with horseradish peroxidase (Cat #1030-05, #1040-05, Southern Biotech), diluted in assay diluent (0.5% BSA and 0.05% Tween 20 in PBS), and incubated for 1 hour at room temperature. After washing, TMB peroxidase substrate (Cat #0410-01, Southern Biotech) was added to each well. Absorbance was read at 450 nm on a microplate reader (BioTek).

Iwanaga N., Chen K., Yang H., Lu S., Hoffmann J.P., Wanek A., McCombs J.E., Song K., Rangel-Moreno J., Norton E.B, & Kolls J.K. (2021). Vaccine-driven lung TRM cells provide immunity against Klebsiella via fibroblast IL-17R signaling. Science immunology, 6(63), eabf1198.

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