Embryos were dechorionated and anaesthetised with 0.16 mg/mL (1x) tricaine methanesulfonate (Sigma). Embryos were then mounted in 0.8% low melting point agarose (Life Technologies) and immersed in E3 buffer with 1x tricaine. When needed, heartbeat was inhibited by changing the medium for E3 buffer with 4x tricaine. Live imaging was performed on an inverted 3i Spinning Disk Confocal using a Zeiss C-Apochromat 63x/1.2 NA water immersion objective, on an upright 3i Spinning Disk Confocal using a Zeiss Plan-Apochromat 63x/1.0 NA water dipping objective, and on an inverted Andor Revolution 500 Spinning Disk Confocal using a Nikon Plan Apo 60x/1.24 NA water immersion objective.
Plan apochromat 63x 1.0 na water dipping objective
Manufactured by Nikon
The Plan-Apochromat 63x/1.0 NA water dipping objective is a high-performance microscope objective designed for advanced imaging applications. It provides a numerical aperture of 1.0 and a magnification of 63x, delivering excellent optical performance and resolution. The objective is optimized for use with water as the immersion medium.
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2 protocols using plan apochromat 63x 1.0 na water dipping objective
1
Zebrafish Embryo Imaging Protocol
2
Zebrafish Embryo Imaging Protocol
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Embryos were dechorionated and anaesthetised with 0.16 mg/mL (1x) tricaine methanesulfonate (Sigma). Embryos were then mounted in 0.8% low melting point agarose (Life Technologies) and immersed in E3 buffer with 1x tricaine. When needed, heartbeat was inhibited by changing the medium for E3 buffer with 4x tricaine. Live imaging was performed on an inverted 3i Spinning Disk Confocal using a Zeiss C-Apochromat 63x/1.2 NA water immersion objective, on an upright 3i Spinning Disk Confocal using a Zeiss Plan-Apochromat 63x/1.0 NA water dipping objective, and on an inverted Andor Revolution 500 Spinning Disk Confocal using a Nikon Plan Apo 60x/1.24 NA water immersion objective.
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