Rats had access to either chow (n=14; 346.0 ±16.5g at start of testing) or 60% HFD (n=15; 513.4 ±39.6g at start of testing) and were given an IP injection of GTS-21 (Sigma-Aldrich) at either a high (10 mg/kg), medium (5 mg/kg), or low (2.5 mg/kg) dose, the GLP-1 receptor agonist, exendin-4 (Ex4; Bachem; 3 μg/kg) as a positive control [27 (link)], or vehicle only (1 ml/kg sterile 0.9% NaCl). GTS-21 doses were selected based on previous work in mice [17 (link)]. Food intake and body weight were measured as described above. Two rats were excluded as statistical outliers (defined below) resulting in a final n=12 for the chow diet condition. For the HFD experiment, four rats were excluded as statistical outliers resulting in a final n=11.
Exendin 4 ex4
Manufactured by Bachem
Exendin-4 (Ex4) is a peptide manufactured by Bachem. It is a synthetic version of a naturally occurring compound found in the saliva of the Gila monster lizard. Exendin-4 functions as a glucagon-like peptide-1 (GLP-1) receptor agonist.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using exendin 4 ex4
1
GTS-21 Dose-Response Effects on Diet-Induced Obesity
2
Insulin and cAMP Measurement Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Rat/Mouse Insulin ELISA Kit was from Linco Research, and the cAMP EIA kit was from Assay Designs. WIN55,212‐2, CP 55,940, arachidonyl‐2′‐chloroethylamide (ACEA) and AM251 were obtained from Cayman Chemical. Exendin‐4 (Ex‐4) was obtained from Bachem. The human CB1R cDNA was amplified by RT‐PCR from human pancreatic RNA (Stratagene), with oligo‐dT (18 bp) for the reverse transcription. The CB1R cDNA was incorporated into a mCerulean‐N1 vector for CB1R‐cerulean with the cerulean epitope.
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