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Lasereo

Manufactured by Fujifilm
Sourced in Japan

The LASEREO is a high-performance lab equipment developed by Fujifilm. It utilizes laser technology for precise and efficient sample analysis. The core function of the LASEREO is to provide accurate and reliable data for research and testing applications.

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11 protocols using lasereo

1

Endoscopic Color Analysis of Barrett's

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EGD was performed using a high-definition EG-L600WR or EG-L600ZW endoscope corresponding to the LASEREO endoscopic system (FUJIFILM, Tokyo, Japan) before the endoscopic treatment. The WLI, BLI, and LCI images of the targeted cases recorded in the filing system were extracted and retrospectively evaluated using a computer to measure the color difference. The color difference between the inside and outside of the BA lesion was examined using endoscopic images taken from almost the same position on the captured image in a fully extended condition without magnification.
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2

Comparative Endoscopic Imaging Techniques

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A video endoscopy system (LASEREO, Fujifilm Corporation, Tokyo, Japan) and high-definition upper gastrointestinal endoscope (EG-L590ZW; Fujifilm Corporation, Tokyo, Japan) were used for WLI and BLI in the BLI-ME group. The structure enhancement of the endoscopic video processor was set to A-mode level 6 or B-mode level 8 for BLI. The color mode was fixed to level C1. A video endoscope system (EVIS ELITE, LUCERA SPECTRUM system, Olympus Co, Tokyo, Japan) and high-resolution upper gastrointestinal endoscope-GIF-H260Z, Olympus Co, Tokyo Japan) were used in the NBI group. The structure enhancement function of the endoscopic video processor was set to A-mode level 8 for WLI and B-mode level 8 for NBI. The color mode was fixed to level 1 for NBI.
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3

Endoscopic Imaging with Enhanced Color Contrast

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An endoscopic system with a laser light source (LASEREO, FUJIFILM, Japan) was used. The system included two lasers with different wavelengths. One was a white-light laser (wavelength 450 ± 10 nm) that provided wide-spectrum, white-light illumination suitable for general observation. The other was a BLI-mode laser (wavelength 410 ± 10 nm) with a short wavelength and a narrow band. The BLI mode delivered high contrast signals which provided information on vessels on the mucosal surface, mucosal irregularities, and deep blood vessels. The intermediate BLI-bright mode, which has a higher white-light intensity ratio, provided a brighter image. The LCI used in the current study is a novel image-enhanced mode, based on the BLI-bright image, capable of additional image processing and enhances separation of the red color to depict the red and white colors more vividly. Because LCI enhances color contrast, differences between even light-colored lesions and surrounding tissue were emphasized, thereby facilitating visualization.
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4

Comparison of Upper GI Endoscopic Systems

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Two different upper GI endoscopic systems manufactured by Olympus Medical Systems Co. and Fujifilm Co. were used in this study. A high-resolution endoscope (GIF-H260Z; Olympus Medical Systems Co., Tokyo, Japan) and a video processor with NBI function (EVIS LUCERA; Olympus Medical Systems Co., Tokyo, Japan) were used for white light imaging (O-WLI) observation and narrow band imaging (NBI) observation. The structure enhancement of the endoscopic video processor was set to B-mode level 3 for O-WLI and B-mode level 8 for NBI. The color mode was fixed at level 1 for NBI.
A high-resolution endoscope (EG-L590ZW; Fujifilm Co., Tokyo, Japan) and a video processor with BLI-bright function (LASEREO; Fujifilm Co., Tokyo, Japan) were used for white light imaging (F-WLI) observation and blue laser imaging-bright (BLI-bright) observation. The structure enhancement of the endoscopic video processor was set to A-mode level 6 for BLI-bright. The color mode was fixed at level C1. The depth of field for the GIF-H260Z and EG-L590ZW endoscope was 7 to 100 mm and 6 to 100 mm, respectively. The field of view for both endoscopes was 140 degrees.
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5

Endoscopic Gastric Atrophy and H. pylori Evaluation

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In the first year, EGD was performed with an EG-L580NW scope and light source LASEREO (FUJIFILM Medical Co., Ltd., Tokyo, Japan). At endoscopic examination, we evaluated the degree of atrophy and H. pylori infection status [6 (link)]. Gastric mucosal atrophy was classified by degree into grades C-0 (none), C-1, C-2, C-3, O-1, O-2, and O-3 according to the endoscopic–atrophic-border scale described by Kimura and Takemoto [9 (link)–11 (link)]. Regarding H. pylori infection status, findings described in the Kyoto classification of gastritis [5 ] such as presence or absence of regularly arranged collecting venules, enlarged gastric folds, nodularity, diffuse and/or spotty redness, and map-like or patchy redness of the gastric mucosa were evaluated. Based on these findings, gastric mucosa was endoscopically classified into non-gastritis (looking like never infected with H. pylori), active gastritis (current H. pylori infection), inactive gastritis (past infection), or undefined (equivocal or status of gastritis difficult to judge). The main diagnostic criteria were gastric mucosal atrophy with diffuse and/or spotty redness for active gastritis; atrophy with map-like redness and/or patchy redness for inactive gastritis; and regularly arranged collecting venules in the lesser curvature of the gastric angle for non-gastritis [6 (link)].
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6

Gastric Cancer Diagnosis and Staging

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All individuals underwent NBI or BLI endoscopy (endoscopes: GIF-Q260, H260, H260Z, H290, HQ290 or H290Z; EG-L590WR or -L600ZW) with an electronic endoscopic system (Elite CV-290: Olympus Medical Systems, Tokyo; LASEREO: Fujifilm Holdings, Tokyo). The histology, macroscopic type, and depth of invasion in the resected materials obtained by ESD and biopsy specimens fulfilled the gastric cancer criteria of the Japanese Gastric Cancer Association
20 (link)
.
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7

Gastric Intestinal Metaplasia Detection

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All procedures were performed with a high-definition GF-L590WR endoscope that was part of the LASEREO endoscopic system (FUJIFILM Co., Tokyo, Japan). The LCI technique used in the present study is a novel image enhanced mode based on BLI-bright image with additional image processing that enhances the color separation of red colors, allowing more vivid visualization of red and white colors.
We selected the gastric antrum, body and angulus for examination in this study. First, after routine observation, the endoscopist used an LCI system to carefully observe the gastric antrum, body and angulus. When a PIM area was detected during non-magnifying observation with LCI imaging, targeted biopsies were subsequently taken from the area. If the suspected area had no PIM on the surface, targeted biopsies were also taken. Currently, there are no standard criteria for GIM in LCI or WL; therefore, any abnormal mucosal change, such as localized discoloration and rough areas, was considered to be indicative of a GIM lesion and biopsies were taken.
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8

Bowel Prep for Colonoscopy

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For bowel preparation, patients ingested 1.5–2 L of polyethylene glycol or 90 mL of a split-dose phospho-soda solution (Fleet; CB Fleet Co, Lynchburg, VA, USA) before the procedure. Two colonoscopists performed all colonoscopy procedures up to the cecum using high-resolution endoscopy (EC-L590ZW/L; Fujifilm, Tokyo, Japan) and a laser light source (Lasereo; Fujifilm).
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9

Laser and LED Endoscopic Systems for Colonoscopy

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The laser endoscopic system (LASEREO; Fujifilm) consisting of an LL‐7000 light source, VP‐7000 processor, and EC‐L600ZP, EC‐L600ZP7, EC‐L600ZP7/L (Fujifilm) colonoscopes and the LED endoscopic system (ELUXEO; Fujifilm) consisting of the BL‐7000 light source, VP‐7000 processor, and an EC‐760ZP‐V/M colonoscope were used in this study. Structural enhancement for the laser‐BLI and the LED‐BLI was set to level B8. Color enhancement was set to level C2 (Table 1).
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10

Laser-enhanced Ultrathin Endoscope Imaging

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An endoscopic system using a laser light source (LASEREO, Fujifilm, Japan) was used. This system has two lasers with different wavelengths. One is a white light laser (wavelength 450 ± 10 nm) providing wide-spectrum white light illumination suitable for general observation. The other is a blue laser imaging mode laser (wavelength 410 ± 10 nm) with characteristics of short wavelength and narrowband. Therefore, in comparison with the endoscope system using the conventional xenon light source, it is feasible to visualize a brighter and higher-resolution image. The ultrathin endoscope (EG-L580NW) used in this system is 5.8 mm in scope outside diameter, 2.4 mm in forceps diameter, and 140° in viewing angle showing markedly improved performance as compared with the previous ultrathin endoscope. It can be used in not only the transoral but also transnasal route. As the conventional endoscope, EG-L590WR, EG-L590ZW, or EG-L600ZW was used.
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