Lncap clone fgc

Murine colon carcinoma CT26, human bladder carcinoma J82, T24, SW1710, and UM-UC-3, breast cancer MCF-7 and T47D, colorectal cancer DLD-1, HCT15, HT29, Lovo, and HCT116, hepatoma HepG2, non-small cell lung cancer NCI-H460, ovary cancer Caov-3, prostate cancer LNCap clone FGC, and renal carcinoma Caki-1, as well as normal HUVEC, intestinal epithelial FHs74Int, and lung fibroblast MRC-5 cell lines were obtained from the National Collection of Authenticated Cell Cultures. Murine colorectal adenocarcinoma MC38 cell lines were purchased from MingzhouBio, Ningbo, Zhejiang, China. The cells were maintained in the logarithmic phase of growth in DMEM, MEM, McCoy’5a, or RPMI medium supplemented with 10% heat-inactivated FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin in an incubator at 37 °C with a humidified atmosphere of 5% CO₂. Cell line identity was validated through short tandem repeat profiling, and routine mycoplasma testing was negative for contamination.

Murine colon carcinoma CT26, human bladder carcinoma J82, T24, SW1710, and UM-UC-3, breast cancer MCF-7 and T47D, colorectal cancer DLD-1, HCT15, HT29, Lovo, and HCT116, hepatoma HepG2, non-small cell lung cancer NCI-H460, ovary cancer Caov-3, prostate cancer LNCap clone FGC, and renal carcinoma Caki-1, as well as normal HUVEC, intestinal epithelial FHs74Int, and lung fibroblast MRC-5 cell lines were obtained from the National Collection of Authenticated Cell Cultures. Murine colorectal adenocarcinoma MC38 cell lines were purchased from MingzhouBio, Ningbo, Zhejiang, China. The cells were maintained in the logarithmic phase of growth in DMEM, MEM, McCoy’5a, or RPMI medium supplemented with 10% heat-inactivated FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin in an incubator at 37 °C with a humidified atmosphere of 5% CO₂. Cell line identity was validated through short tandem repeat profiling, and routine mycoplasma testing was negative for contamination.

The human prostate cancer cell lines, PC3, DU145, and LNCaP Clone FGC, were obtained from the National Collection of Authenticated Cell Cultures (Shanghai, China) and were STR certified. PCR was used to detect Mycoplasma in the culture medium, and the passage time of the cells was not more than 6 months. The cell lines were cultured in RPMI 1640 (HyClone, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and grown at 37 °C, 5% CO₂. The antibodies included TACR2 (Proteintech, 25270-1-AP), beta-Catenin (Proteintech, 51067-2-AP), GAPDH (Cell Signaling Technology, 5174S), Cyclin D1 (Cell Signaling Technology, 55506S), Lamin B1 (Cell Signaling Technology, 13435S).