Mouse anti ecad

Manufactured by BD

Sourced in United Kingdom

Mouse anti-Ecad is a laboratory product designed for use in cell biology research. It serves as a tool for the detection and analysis of E-cadherin, a cell adhesion protein, in experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti met, by Abcam (2 mentions) Chicken anti vimentin, by BioLegend (2 mentions) Rabbit anti zo 1, by Thermo Fisher Scientific (2 mentions) Mouse anti n cad, by BD (2 mentions) Ncl ki67p, by Santa Cruz Biotechnology (1 mentions) Goat anti olig2, by R&D Systems (1 mentions) Rabbit anti ki67 polyclonal antibody, by Leica Biosystems (1 mentions) Rabbit anti olig2 igg, by Merck Group (1 mentions) Chick anti gfap, by Merck Group (1 mentions) Mouse anti s100β, by Merck Group (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Rabbit anti wave2, by Cell Signaling Technology (1 mentions) Rabbit anti e cad, by Cell Signaling Technology (1 mentions) Mouse anti arp3, by Merck Group (1 mentions) Rabbit anti myc, by Merck Group (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions) Alexa fluor 594 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Goat anti chicken alexa fluor 594, by Thermo Fisher Scientific (1 mentions)

6 protocols using mouse anti ecad

Comprehensive Antibody Characterization for Cell Lineage Tracing

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in this study are in the followings: mouse anti-E-cad (BD Transduction Laboratories 61081, 1:250); chick anti-GFAP (Millipore, 1:5,000); chick anti-GFP IgY (Abcam ab13970, 1:5,000); rabbit anti-GLAST polyclonal antibody (Covalab (France) PA-INS, 1:1,000); rabbit anti-Ki67 polyclonal antibody (Novocastra, NCL-Ki67p, 1:1,000); goat anti-NeuroD antibody (N-19; Santa Cruz, sc-1,084, 1:1,000); rabbit anti-Olig2 IgG (Millipore, AB9610, 1:1,000); goat anti-Olig2 (R&D systems, 1:1,000); mouse anti-PCNA (Novocastra, PC-10, 1:100); rabbit anti-PSmad3(S423/S425) polyclonal antibody (Millipore 07-1389, 1:1,000); mouse anti-phospho-vimentin IgG (MBL, D076-3S, 1:1,000); goat anti-Prox1 IgG (R&D systems, 1:1,000); mouse anti-S100β (Sigma, 1:1,000); rabbit Sox2 antibody (Millipore, 1:1,000); goat anti-Sox2 IgG (R&D systems, 1:1,000); goat anti-Sox9 polyclonal antibody (R&D systems, 1:1,000).

Ohyama K., Shinohara H.M., Omura S., Kawachi T., Sato T, & Toda K. (2023). PSmad3+/Olig2− expression defines a subpopulation of gfap-GFP+/Sox9+ neural progenitors and radial glia-like cells in mouse dentate gyrus through embryonic and postnatal development. Frontiers in Neuroscience, 17, 1204012.

+ Open protocol

+ Expand

Immunofluorescence and Western Blot Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies: goat anti-Neogenin (C-20) (immunofluorescence (IF), 1:50; western blotting (WB), 1:200; Santa Cruz Biotechnology, #sc-6536); rabbit-anti-Neogenin (H-175) (IF, 1:50; Santa Cruz Biotechnology, #sc-15337); mouse anti-Ecad (IF, 1:250; WB, 1:1,000; BD Transduction Laboratories, #610182); rabbit anti-Ecad (IF, 1:250; Cell Signaling Technology, #3195); rabbit-anti GAPDH (WB, 1:2,500; Abcam, #ab9485); mouse anti-Myc (IF, 1:500; Sigma-Aldrich, #M4439, clone number 9E10); rabbit anti-Myc (IF, 1:500; Millipore, #06-549); rabbit anti-WAVE2 (IF, 1:50; WB, 1:1,000; Cell Signaling Technology, #3659); mouse anti-Arp3 (IF, 1:200; WB, 1:400; Sigma-Aldrich, #A5979); rabbit anti-Sra1 (IF, 1:200; Millipore, #07-531); rabbit anti-PKC zeta (IF, 1:250; Santa Cruz Biotechnology, #sc-216); and mouse anti-ZO-1 (IF, 1:500; Invitrogen, #33-9100). Secondary antibodies were conjugated to Alexa Fluor 488, 546, 568 or 647 (IF, 1:500; Molecular Probes). Phalloidin conjugated to Alexa Fluor 546 was used for F-actin labelling (IF, 1:500; Molecular Probes, #A22283).

Lee N.K., Fok K.W., White A., Wilson N.H., O'Leary C.J., Cox H.L., Michael M., Yap A.S, & Cooper H.M. (2016). Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension. Nature Communications, 7, 11082.

+ Open protocol

+ Expand

Immunofluorescence Analysis of HBMEC and HIBCPP Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence analysis of HBMEC grown in chamber slides and HIBCPP cells cultivated in the inverted cell culture insert model was performed as previously described [29 (link)]. The following antibodies were used: primary antibodies: chicken anti-vimentin (BioLegend, San Diego, CA, USA), goat anti-Met (Abcam, Cambridge, UK), mouse anti-Ecad (BD, Franklin Lakes, NJ, USA), and rabbit anti-ZO-1 (Invitrogen, Carlsbad, CA, USA); secondary antibodies: goat anti-chicken Alexa Fluor^® 594, donkey anti-goat Alexa Fluor^® 594, goat anti-mouse Alexa Fluor^® 594, chicken anti-rabbit Alexa Fluor^® 488, and donkey anti-rabbit Alexa Fluor^® 488 (all Invitrogen, Carlsbad, CA, USA). Nuclei were stained with 4′-6-diamidino-2-phenylindole dihydrochloride (DAPI) (1:50,000 in PBS/1% BSA) (Merck, Darmstadt, Germany). Densitometric analysis of Western blot bands normalized to actin was performed using the ImageJ software 1.53e [48 (link)].

Banovic F., Schulze S., Abu Mraheil M., Hain T., Chakraborty T., Orian-Rousseau V., Moroniak S., Weiss C., Ishikawa H., Schroten H., Adam R, & Schwerk C. (2022). Different Involvement of Vimentin during Invasion by Listeria monocytogenes at the Blood–Brain and the Blood–Cerebrospinal Fluid Barriers In Vitro. International Journal of Molecular Sciences, 23(21), 12908.

+ Open protocol

+ Expand

Western Blot Protein Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To obtain protein extracts for Western blotting, cells were washed with PBS twice and lysed in RIPA buffer after the removal of all excess PBS. Proteins of 10 to 20 µg of protein extract were separated on 4–12% Bis-Tris (Invitrogen, Karlsruhe, Germany) and subsequently transferred onto nitrocellulose membranes via electroblotting. Membranes were incubated for 1 h in blocking solution (5% milk powder solution in dH₂O), and proteins were detected with the following antibodies: chicken anti-vimentin (BioLegend, San Diego, CA, USA), goat anti-Met (Abcam, Cambridge, UK), mouse anti-Ecad (BD, Franklin Lakes, NJ, USA), and rabbit anti-ZO-1 (Invitrogen, Carlsbad, CA, USA). The Immobilon Western Kit (Millipore, Schwalbach, Germany) was used for the visualization of detected proteins.

+ Open protocol

+ Expand

Pluripotency and Lineage Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: mouse anti-Oct4 (Santa Cruz), rabbit anti-Nanog54 (link), anti-Sox2 (Calbiochem), rabbit anti-E-cad (extracellular)55 (link), mouse anti-E-cad (intracellular, BD Bioscience), mouse anti-N-cad (BD Bioscience), mouse anti-N-cad (Life Technologies), rat anti-ZO-156 (link), rat anti-pan-Laminin57 , goat anti-T-brachyury (Santa Cruz), goat anti-Sox17 (R&D Systems), rabbit anti-pH3 (Cell Signaling), rabbit anti-H3 (Abcam), mouse anti-β-catenin (BD Bioscience), mouse anti-Ezrin (Cell Signaling) and rabbit anti-pSMAD1-5-8 (Cell Signaling).

Basilicata M.F., Frank M., Solter D., Brabletz T, & Stemmler M.P. (2016). Inappropriate cadherin switching in the mouse epiblast compromises proper signaling between the epiblast and the extraembryonic ectoderm during gastrulation. Scientific Reports, 6, 26562.

+ Open protocol

+ Expand

Comprehensive Immunohistochemistry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against GPx2 (AB137431), HIF1α (AB228649), NOTCH1 (AB52627), pAMPK (staining, AB23875) were obtained from Abcam. Rabbit polyclonal anti TWIST (sc-15393), was obtained from Santa Cruz Biotechnology. Rat anti KRT8 (staining, Cat# TROMA-I; RRID: AB_531826) was purchased from DSHB. Rabbit anti p63 (staining, 39692), VIM (staining and WB, 5741), SLUG (9585), E-CAD (staining and WB, 3195), pAMPK(WB, 2535), and mouse anti SNAI1 (3895) were from Cell Signaling and β-actin antibody was from Sigma. Rabbit anti KRT14 antibody (staining, 905304) was from Biolegend. Guinea pig anti KRT14 (staining, Cat#. GP-CK14) was from Progen. Mouse anti E-CAD (WB and staining, 610182) and mouse anti N-cad (staining, 610920) was from BD Transduction Labs. Rabbit anti-SLC2A1(GLUT1) antibody (WB, MBS9126610) was purchased from BioSource. Mouse anti-SLC2A1(GLUT1) (staining, NBP-75785-100ug) was purchased from Novus Biologicals. Alexa Fluor 405, 488, 594 goat anti mouse or rabbit or rat, Alexa Fluor 647 donkey anti guinea pig were from Invitrogen. Echinomycin was obtained from Tocris Bioscience.

Ren Z., Dharmaratne M., Liang H., Benard O., Morales-Gallego M., Suyama K., Kumar V., Fard A.T., Kulkarni A.S., Prystowsky M., Mar J.C., Norton L, & Hazan R.B. (2024). Redox signalling regulates breast cancer metastasis via phenotypic and metabolic reprogramming due to p63 activation by HIF1α. British Journal of Cancer, 130(6), 908-924.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!