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8 protocols using anhydrous alcohol

1

Synthesis and Characterization of Zeolites

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The quartz
capillaries (internal diameter (i.d.) 0.53 and 0.32 mm, length 100
mm) were purchased from Dalian Zhonghuida Scientific Instrument Corporation.
The chemical reagents included tetrapropylammonium hydroxide solution
(TPAOH, ∼50 wt % in water), tetraethyl orthosilicate (TEOS,
wt % ≥99.5%), sodium aluminate (NaAlO2, wt % ≥99.0%),
sodium hydroxide (NaOH, wt % ≥96.0%), anhydrous alcohol (EtOH,
wt % ≥99.7%), ammonium acetate (NH4Ac, wt % ≥98.0%),
sodium sulfate (Na2SO4, wt % ≥99.0%),
acetone (marked as ACT, wt % ≥99.5%), glycerol (marked as GCR,
wt % ≥99.0%), and N,N-dimethylformamidel
(DMF, wt % ≥99.5%) were purchased from Sinopharm Chemical Reagents
Co., Ltd. (Shanghai, China). The used chemical coupling agent of (3-aminopropyl)
trimethoxysilane (APTMS, ∼97 wt %) was purchased from Aladdin
Industrial Corporation (Shanghai, China). All the above used chemical
reagents were analytical reagent (AR) and used as received without
further purification. The used deionized water (DI water, 18.2 MΩ)
was home-made.
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2

Auricularia polytricha Cultivation Protocol

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Auricularia polytricha No.10 strain was purchased from Jiangsu Tianda Institute of Edible Fungi (Jiangsu, China).
Bovine serum albumin (BSA), Coomassie brilliant blue G–250, and ascorbic acid were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Concentrated sulfuric acid, phosphoric acid, salicylic acid, trichloroacetic acid (TFA), anhydrous alcohol, acetone, anhydrous ether, glucose, sucrose, fructose, maltose and lactose, urea, peptone, ammonium sulfate, yeast and corn starch, neutral protease, 30% hydrogen peroxide (H2O2), phenol, Vitamin C (Vc) sodium hydroxide, ferrous sulfate (FeSO4), ferric chloride (FeCl3), sodium dihydrogen phosphate, disodium hydrogen phosphate, and potassium ferricyanide [K3Fe(CN)6] were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The monosaccharide Standards including fucose (Fuc), rhamnose (Rha), arabinose (Ara), mannose (Man), galactose (Gal), glucose (Glc), xylose (Xyl), fructose (Fru), galacturonic acid (GalA), glucuronic acid (GlcA), 2,2–diphenyl–1–picrylhydrazyl (DPPH), and potassium bromide (KBr, Spectrum pure grade) were purchased from Sigma Chemical Co. (St. Louis, MO, United States). All chemicals and solvents used in the current work were of analytical grade.
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3

Synthesis of Silver Nanowire Composites

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Silver nanowires suspension (Ag NWs, CST-NW-S30) in ethyl alcohol was purchased from Suzhou Coldstones Technology Co., Ltd., Suzhou, China. Rhodamine 6 g (R6G, ≥95%), zinc chloride (ZnCl2), N,N-dimethylformamide (DMF), and sodium hydroxide (NaOH) were obtained from Shanghai Aladdin Reagent Co., Ltd, Shanghai, China. Anhydrous alcohol (99%) and Millipore ultrapure water were purchased from Sinopharm Chemical Reagent Co., Ltd, Shanghai, China. Pure acetone and Kelian 25# transformer mineral oil (41.6% naphthenic, 50.0% paraffinic, and 8.4% aromatic) were purchased from the Chuanrun Lubricant Company. All reagents were of analytical grade and used without further purification. Water used in all experiments was purified on a Millipore system (18.2 MΩ cm).
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4

Synthesis of Mesoporous Silica Nanoparticles

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The chemical reagents included
tetraethyl orthosilicate (TEOS, wt % ≥99.5%), aqueous ammonia
(NH4OH, wt % ≥25%), anhydrous alcohol (EtOH, wt
% ≥99.7%), acetone (marked as ACT, wt % ≥99.5%), and
glycerol (marked as GCR, wt % ≥99.0%) were purchased from Sinopharm
Chemical Reagents Co., Ltd. (Shanghai, China). The used hexadecyl
trimethyl ammonium bromide (CTAB, wt % ≥99%) and ammonium tungstate
(wt % ≥98.5%) were purchased from Aladdin Industrial Corporation
(Shanghai, China). All the above used chemicals were of analytical
reagent (AR) grade and used as received without further purification.
The used deionized water (DI water, 18.2 MΩ) was homemade.
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5

Synthesis of Cobalt Oxalate Nanoparticles

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Sodium hydroxide (NaOH) pellets (ACS reagent, ≥97.0%), oxalic acid (H2C2O4, 98%) powder, cobaltous nitrate hexahydrate (Co(NO₃)₂·6H₂O, reagent grade, 98%) flakes were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anhydrous alcohol (99%) was purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Ultrapure water (18.2 MΩ cm) was purified on a Milli−Q Advantage A10 (Millipore, USA). All reagents were used without further purification.
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6

Rare Earth Chloride Synthesis Protocol

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All chemical reagents were obtained from the commercial institution and used without further purification. The rare earth (RE) chlorides including LuCl3•6H2O (99.9%), GdCl3•6H2O (99.9%), YbCl3•6H2O (99.9%), and ErCl3•6H2O (99.9%) were obtained from QingDa elaborate Chemical Reagent Co. Ltd (Shandong). NaOH, NaF, anhydrous alcohol, toluene, oleic acid (OA), IGEPAL CO-520, dopamine hydrochloride, ammonium hydroxide, 1,2-dichloroethane (DCE), IR-26 dye, cyclohexane, and other reagents were all purchased from Sinopharm Chemical Reagent Co., China.
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7

Synthesis of Silica-Coated Polymer Microspheres

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The silica precursor tetraethylorthosilane (TEOS) was purchased from Alfa Aesar (Beijing, China). The template polymer microspheres are a polymer of glycidyl methacrylate (GMA) cross-linked with ethylene glycol dimethacrylate (EGDMA) supplied by Nano-Micro Technology Company (Jiangsu, China). Ferric chloride hexahydrate (FeCl3 · 6H2O), sodium oleate, trimethylamine (TMA) hydrochloride, sodium hydroxide, ammonium hydroxide (28% aqueous solution), and ethanol were purchased from Shanghai Chemical Reagent Corp. (Shanghai, China). Hexanes, chloroform, sodium borohydride (NaBH4), 4-nitrophenol (4-NP), and 1-octadecene were purchased from Alfa Aesar. Anhydrous alcohol and chloroauric acid tetrahydrate (HAuCl4 · 4H2O) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) Water was purified by distillation followed by deionization using ion exchange resins. Other chemicals were of analytical grade and used without any further purification.
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8

Synthesis and Characterization of Lead Nanomaterials

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GO nanoflakes were purchased from Xianfeng Nanotechnology, Inc. (Nanjing, China). Manganese sulfate monohydrate (MnSO4·H2O), bismuth nitrate pentahydrate (Bi(NO3)3·5H2O), lead nitrate (Pb(NO3)2), potassium peroxydisulfate (K2S2O8), potassium sulfate (K2SO4), N, N-dimethylformamide (DMF), potassium ferri/ferro-cyanide (K3/4[Fe(CN)6]), sodium acetate (NaAc), acetic acid (HAc), concentrated H2SO4, and anhydrous alcohol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents were of analytical grade and directly used as received. Water samples were collected from a local lake and from our laboratory. 0.3312 g of Pb(NO3)2 was completely dissolved in 100 mL of 0.1 M HAc-NaAc buffer (pH = 5.5) to prepare a 0.01 M Pb2+ stock solution. A series of standard solutions of Pb2+ with different concentrations were prepared by appropriately diluting the stock solution with the 0.1 M HAc-NaAc buffer (pH = 5.5). Deionized water (DI water, resistivity of 18.2 MΩ·m) was used for all experiments.
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