RIP assay was performed using the Magna RI RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA) according to the manufacturer's instructions. The magnetic beads (Bio-Rad, Hercules, CA, USA) were incubated with Argonaute-2 antibody (Anti-Ago2) or Immunoglobulin G antibody (Anti-IgG). Then, the HL-1 cell extract was incubated with RIP buffer containing magnetic beads conjugated to human anti-AGO2 antibody (Millipore, Billerica, MA, USA) and IgG control (Millipore, Billerica, MA, USA). The coprecipitated RNA was purified and quantified by qRT-PCR. RNA integrity was assessed with an Agilent 2100 Bioanalyzer.
Magna ri rna binding protein immunoprecipitation kit
Manufactured by Merck Group
Sourced in United States
The Magna RI RNA-Binding Protein Immunoprecipitation Kit is a tool for the isolation and purification of RNA-binding proteins from cell or tissue samples. The kit provides reagents and protocols to perform RNA-binding protein immunoprecipitation experiments.
Automatically generated - may contain errors
Lab products found in correlation
Magnetic bead, by Merck Group (2 mentions)
Magnetic beads, by Bio-Rad (2 mentions)
Control igg, by Merck Group (2 mentions)
Human anti ago2 antibody, by Merck Group (2 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Rip buffer, by Merck Group (1 mentions)
Sepharose beads, by Bio-Rad (1 mentions)
Sepharose beads, by Merck Group (1 mentions)
4 protocols using magna ri rna binding protein immunoprecipitation kit
1
RIP Assay for Argonaute-2 Binding
2
Argonaute-2 RNA-Binding Protein IP
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Magna RI RNA‐Binding Protein Immunoprecipitation kit (Millipore, Billerica, MA, USA) was applied in this study. Sepharose beads (Bio‐Rad, Hercules, CA, USA) were incubated with Argonaute‐2 antibody (Anti‐Ago2) or Immunoglobulin G antibody (Anti‐IgG). NSCLC cells were disrupted using RIP buffer (Millipore), and then incubated with precoated Sepharose beads. The expression of relevant RNAs was measured by qRT‐PCR.
3
HAND2-AS1 and miR-590-3p Interaction
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RIP assay was utilized to investigate the connection of HAND2-AS1 and miR-590-3p using Magna RI RNA-Binding Protein Immunoprecipitation Kit (Millipore, Sigma Aldrich, Merck, Billerica, MA, USA). RIP buffer was used to incubate cultured cells and treated with anti-Ago2 antibody and control IgG. Samples were then treated with Proteinase K and subjected to RT-qPCR analysis to analyze HAND2-AS1 and miR-590-3p levels.
4
Argonaute-2 RNA Immunoprecipitation Assay
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RIP assay was performed using the Magna RI RNA-Binding Protein Immunoprecipitation kit (Millipore, Billerica, MA, USA) according to the manufacturer's instructions. The magnetic beads (Bio-Rad, Hercules, CA, USA) were incubated with Argonaute-2 antibody (Anti-Ago2) or Immunoglobulin G antibody (Anti-IgG). Then, HL-1 cells extract was incubated with RIP buffer containing magnetic beads conjugated to human anti-AGO2 antibody (Millipore, Billerica, MA, USA) and IgG control (Millipore, Billerica, MA,USA). The coprecipitated RNA was puri ed and quanti ed by qRT-PCR. RNA integrity was assessed with an Agilent 2100 Bioanalyzer.
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