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Calcium hydroxide ca oh 2

Manufactured by Merck Group
Sourced in Germany, United States

Calcium hydroxide (Ca(OH)2) is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is insoluble in water but soluble in acids. Calcium hydroxide has a molecular weight of 74.09 g/mol and a melting point of 580°C. It is a basic compound that can be used in a variety of laboratory applications.

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8 protocols using calcium hydroxide ca oh 2

1

Synthesis of Calcium Silicate Hydrate

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Calcium silicate hydrate C–S–H
gel (composed of nanometric colloidal particles) was obtained following
a procedure adapted from Myers and co-workers.49 (link) MilliQ water was boiled in a flask to remove the CO2 and then cooled down for 10–15 min. Calcium oxide
CaO was prepared by calcination of calcium hydroxide Ca(OH)2 (Merck) at 800 °C for 24 h. We subsequently mixed all ingredients—MilliQ
water, colloidal silica SiO2 (Ludox TM50, Merck), and calcium
oxide CaO, in this order—in a plastic container, with a water-to-solid
mass ratio (w/s) of 45 and a Ca/Si = 1. The container was immediately
sealed, placed on a roller (IKA Roller 6 basic, Werke GmbH and Co.
KG) for a gentle stirring, and flushed with nitrogen for 20 min through
two small holes made in the cover. The two holes were then sealed,
and the container was kept on the roller to assure proper solution
mixing and aging. After 1 week, the solution was centrifuged to obtain
the gel. The final water-to-solid ratio was around 2, and this could
slightly change due to the final solution centrifugation step. This
procedure was validated by X-ray diffraction (XRD), which confirmed
the presence of pure calcium silicate hydrate. The initial pH of the
C–S–H suspension (at w/s = 3) was 12.2, which decreased
slightly to 12.1 within 5 h due to carbonation.50 (link)
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2

Phenol and Calcium Hydroxide Analysis

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The chemicals used in this study, such as phenol (99.99% purity), and calcium hydroxide (Ca(OH)2) were analytical grade purchased from Merck, Germany. All require solutions were prepared using distilled water.
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3

Analytical Grade Chemicals Protocol

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All the chemicals used in this research were analytical grade. Phosphoric acid (H3PO4), calcium hydroxide (Ca(OH)2), and NH4OH were procured from E-Merck Germany and no further purification was required. Deionized (DI) water was used throughout the work.
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4

Pseudomonas aeruginosa Infection Model

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P. aeruginosa PAO1 strain (from ATCC 15692, Manassas, USA), was used to prepare bacteria-loaded agar beads. Ciprofloxacin base (CIP) powder (purity ≥98,0%), copper hydroxide Cu(OH)2, hyaluronic acid (HA) sodium salt from Streptococcus equi, calcium hydroxide Ca(OH)2, formic acid and ammonium carbonate (NH4)2CO3 were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France).
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5

Hydroxyapatite Synthesis and Antibiotic Modification

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The reagents used for the synthesis of hydroxyapatite, including phosphoric acid (HNO3), calcium hydroxide (Ca(OH)2) and ammonia water (NH4OH, 25%), were purchased from Sigma-Aldrich (Darmstadt, Germany). The antibiotic used for the modification was clindamycin hydrochloride, purchased from Sigma-Aldrich (Darmstadt, Germany). The mobile phase used for HPLC was a combination of acetonitrile (CH3CN) from Honeyweel (Seelze, Germany) and potassium dihydrogen phosphate (KH2PO4) from DOR-CHEM (Krakow, Poland). Phosphate-Buffered Saline Tablets were purchased from OXOID (Basingstoke, UK). A Hydrolab model HLP 5sp unit was used to obtain demineralized water for all solutions.
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6

Cartilage Tissue Engineering Reagents

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Sodium hydroxide (NaOH, CAS# 1310-73-2) was obtained from Showa-Denko (Tokyo, Japan). Orthophosphoric acid (H3PO4, CAS# 7664-38-2) was obtained from J.T. Baker (Center Valley, Radnor, PA, USA). Hydrochloric acid (HCl, CAS# 7647-01-0), calcium hydroxide (Ca(OH)2, CAS# 1305-62-0), glutaraldehyde (GA, CAS# 111-30-8), kartogenin (KGN; CAS# 4727-31-5), metformin (MET; CAS# 1115-70-4), and gelatin (CAS# 9000-70-8) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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7

Synthesis of Antimicrobial Cotton Gauze

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Scoured and bleached weave cotton gauze samples were produced by the El-Nasr Spinning, Weaving, and Dyeing Company, Mahala, Egypt. Absolute ethanol (C2H5OH, 99%, ADWIC), glacial acetic acid (CH₃COOH, 99%, Sigma-Aldrich, St. Louis, MO, USA), phosphoric acid (H3PO4, 85 wt.% in H2O, Sigma-Aldrich), calcium hydroxide (Ca(OH)2, ≥96%, Sigma-Aldrich), and ammonium hydroxide (NH4OH, 28% NH3 in H2O, Sigma-Aldrich) were used for the synthesis of HAp NPs. Low molecular weight Cs (MWt = 30 KDa and 97% deacetylation, Acros Organics, Fair Lawn, NJ, USA), sodium hydroxide (NaOH, ≥97.0% pellets, Sigma-Aldrich), and monochloroacetic acid (C2H3ClO2, 99%, Sigma-Aldrich) were used for the cationic and anionic modification of the cotton gauze samples, respectively. Silver nitrate (AgNO3, 99%, Sigma-Aldrich), phenolphthalein (C20H14O4, 98%, Merck, Kenilworth, NJ, USA), and natural ginger oil (Pure Grade, Sigma Aldrich) were used for Ag NPs phytosynthesis.
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8

Ciprofloxacin-Hyaluronic Acid Formulation

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Ciprofloxacin (CIP) powder (purity ≥ 98,0 %), high molecular weight (1.5 to 1.8 x 10 6 Da), hyaluronic acid sodium salt from Streptococcus equi (HA), calcium hydroxide Ca(OH) 2 and ammonium carbonate (NH 4 ) 2 CO 3 were purchased from Sigma Aldrich (Ireland). Buffer concentrate pH 5 (Titrisol) was purchased from Merck (Germany) and Hank's Balanced Salt Solution (HBSS) was purchased from Gibco (France). Deionised water was produced by a Purite Prestige Analyst HP water purification system (Millipore, Carrigtwohill, Ireland). HPLC grade acetonitrile was purchased from Sigma-Aldrich (Ireland).
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