The iSCAMS measurement was carried out using a Refeyn TwoMP mass photometer (Refeyn Ltd.). Cleaned coverslips were assembled into flow chambers. All the buffers used for analysis were filtered through a syringe filter with 0.45-μm pore size (Anotop 10, Whatman). For measurements, all samples were freshly diluted from stock solutions. Sample proteins were diluted to the final concentration of 20 nM in 20 mM HEPES (pH 7.5) buffer containing 200 mM or 400 mM NaCl. The protein solution was loaded into the sample well after finding the focus with the buffer. Obtained data were processed with the DiscoverMP program (Refeyn Ltd.)
Discovermp program
Manufactured by Refeyn
The DiscoverMP program is a software tool that enables the analysis and characterization of macromolecular complexes. It provides analytical capabilities for processing and interpreting data obtained from mass photometry, a technique developed by Refeyn for measuring the mass of single molecules and macromolecular complexes in solution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using discovermp program
1
iSCAMS Mass Photometry of Proteins
2
Mass Photometry Analysis of Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The iSCAMS measurement was carried out using a Refeyn TwoMP mass photometer (Refeyn Ltd.). Cleaned coverslips were assembled into flow chambers. All the buffers used for analysis were filtered through a syringe filter with 0.45-μm pore size (Anotop 10, Whatman). For measurements, all samples were freshly diluted from stock solutions. Sample proteins (100 nM) were diluted to the final concentration of 20 nM in 20 mM HEPES (pH 7.5) buffer containing 200 mM or 400 mM NaCl. The protein solution was loaded into the sample well after finding the focus with the buffer. Obtained data were processed with the DiscoverMP program (Refeyn Ltd.)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!