C2C12 myotubes were washed twice with PBS, and then incubated with oil red O working solution (Servicebio, China) at 37°C for 10 minutes. Used a photography microscope (Nikon, Japan) to select the target area for imaging, and then used Image Pro Plus software for analysis.
Oil red o working solution
Manufactured by Wuhan Servicebio Technology
Sourced in China
Oil red O working solution is a staining reagent used in laboratory procedures. It is a lipophilic dye that selectively stains neutral triglycerides and lipids.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using oil red o working solution
1
Quantifying C2C12 Myotube Lipid Content
2
Histological Assessment of Liver Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver sections were submerged in 4% paraformaldehyde for 24 h, followed with a standard hematoxylin and eosin (H&E) staining procedure. Oil red O staining were performed in liver tissues frozen in OCT and stained in oil red O working solution (Servicebio technology, Wuhan, China). All slides were photographed using a Leica DM2000 light microscope (Leica Microsystems, Inc.; magnification, x100 or x200).
3
Oil Red O Staining of Myotubes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After intervention, the myotubes were washed with PBS twice and then incubated with Oil Red O working solution (Servicebio) for 15 minutes at 37°C. The cells were visualized by light microscopy (Olympus).
4
Lipid and Nuclei Staining in Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The liver tissue samples were embedded with the optimum cutting temperature compound for 10 min and sectioned with a freezing microtome. Frozen sections of liver were fixed with 4% paraformaldehyde for 15 min, stained with oil red O working solution (Servicebio, Wuhan, China) for 10 min away from light, and rinsed with water. After differentiation in 75% ethyl alcohol, the liver cell nuclei were re-stained with hematoxylin (Servicebio, Wuhan, China) for 5 min, rapidly differentiated with hydrochloric acid alcohol, and returned to blue in an ammonia solution. The lipids and nuclei were stained red and blue, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!