Silver acetate was purchased from Sigma–Aldrich Ltd. Silver acetate stocks were prepared by dissolving in sterile distilled water and were stored protected from light at room temperature.
Silver acetate
Manufactured by Merck Group
Sourced in United States, Germany
Silver acetate is a chemical compound with the formula CH3COOAg. It is a white, crystalline solid that is used as a reagent in various chemical applications.
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Lab products found in correlation
Silver nitrate, by Merck Group (5 mentions)
Oleic acid, by Merck Group (5 mentions)
Oleylamine, by Merck Group (4 mentions)
Sodium borohydride, by Merck Group (4 mentions)
Indium 3 acetate, by Merck Group (3 mentions)
Polyvinylpyrrolidone, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Formic acid, by Merck Group (3 mentions)
Ethanolamine, by Merck Group (3 mentions)
Hexane, by Merck Group (2 mentions)
Hydroquinone, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Cetyltrimethylammonium chloride, by Merck Group (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
Citric acid, by Merck Group (2 mentions)
Gold 3 chloride trihydrate, by Merck Group (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions)
Na2so4, by Merck Group (2 mentions)
33 protocols using silver acetate
1
Preparation of Silver Acetate Solutions
2
Silver-Amine Complex Formation Protocol
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0.085 g of silver acetate (98 %, for synthesis, Merck, abbreviation: Ag(ac)) was dispersed in 20 ml ethanol (absolute, VWR) under stirring. The amine ligands were added dropwise in a 1:5 molar ratio (or 1:10 ratio) to the silver acetate and stirred until complete dissolution occurs, and a clear colorless solution is obtained. The employed amine ligands include hexylamine (99 %, Acros Organics), ethanolamine (>99.5 %, Sigma-Aldrich), 2-amino-2-methyl-1-propanol (> 99.0 %, Fluka), ammonia (25 % solution in water, pro analysis, Merck), morpholine (>99 %, for synthesis, Merck) and pyridine (99 %, extra pure, Acros Organics). Subsequently, formic acid (98 %, pro analysis, Sigma-Aldrich) was added to the mixture in a 1:1 molar ratio with silver acetate. This addition yields a miniscule amount of precipitation, which re-dissolves upon continuous stirring. The solution was transferred into a 50 ml volumetric flask and ethanol was added to obtain a 0.01 M silver solution.
3
Synthesis of Silver Carboxylates from Fatty Acids
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Octanoic acid (99%) (OctAc), lauric acid (99%) (LAc), acetic acid (AcAc), 1-octylamine (99%) (OA), 1-dodecylamine (99%) (DDA), silver nitrate (99%), silver acetate (99%) (AgOAc), and sodium hydroxide (NaOH) were purchased from Aldrich or Alfa Aesar and used as received. Solvents (acetone, cyclohexane, and methanol) were distilled prior to use; water was triply distilled. Silver laureate (AgL) was prepared by a one-pot synthesis by adding aqueous solution of NaOH (0.99 equiv. in 2 mL) to the 14-mmol dispersion of lauric acid (2.8 g/20 mL) in hot water (80 °C). The molar amount of NaOH was 1% lower than that of the acid in order to avoid the reaction of excess alkali with AgNO3. Then, an aqueous solution of AgNO3 (0.56 g in 20 mL of water) was added to the vigorously stirred solution. The resulting silver carboxylate in the form of white precipitate was collected, washed with water (3×), and dried at 50 °C overnight to give white solid in quantitative yield. In a similar way, silver octanoate (AgOct) was prepared.
4
Synthesis of h-BN Nanomaterials
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h-BN powder (size -10P, Lot HZ010PA4.$06) was provided by UK Abrasives. Silver acetate (99%) and DMF were purchased from Aldrich. All chemicals and solvents were used as received.
5
Synthesis of Nanomaterials via Solvent Reactions
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Antimony acetate (99.99%, Aldrich), benzoyl
bromide (99.9%, Aldrich), benzoyl chloride (99.9%, Alfa Aesar), bismuth
acetate (99.99%, Aldrich), cesium acetate (99.9%, Aldrich), indium(III)
acetate (99.99%, Aldrich), oleic acid (90%, Aldrich), oleylamine (70%,
Aldrich), silver acetate (99.99%, Aldrich), o-xylene
anhydrous (97%, Aldrich), and hexane (97%, Aldrich) were used as purchased
without further purification.
bromide (99.9%, Aldrich), benzoyl chloride (99.9%, Alfa Aesar), bismuth
acetate (99.99%, Aldrich), cesium acetate (99.9%, Aldrich), indium(III)
acetate (99.99%, Aldrich), oleic acid (90%, Aldrich), oleylamine (70%,
Aldrich), silver acetate (99.99%, Aldrich), o-xylene
anhydrous (97%, Aldrich), and hexane (97%, Aldrich) were used as purchased
without further purification.
6
Synthesis and Characterization of Silver Compounds
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Four types of Ag compounds were used: Silver acetate (C2H3O2Ag; 99%), silver tetrafluoroborate (AgBF4; 98%), silver nitrate (AgNO3; >99.9%), and silver phosphate (Ag3PO4; 98%). Silver acetate, silver tetrafluoroborate, and silver phosphate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Silver nitrate was purchased from Kojima Chemicals (Saitama, Japan). Formic acid (98%) was purchased from Junsei (Tokyo, Japan). Gelatin powder (type A from porcine skin) was purchased from Sigma-Aldrich (St. Louis, MO, USA). An aqueous solution of glutaraldehyde (50%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Glycine was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were used without further purification.
7
Synthesis of Organic Compounds
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Toluene (99.8%), methyl alcohol (99.9%), and nitric acid (68.0–70.0%) were purchased from Samchun. Tetrahydrofuran (THF) was obtained from Duksan. Hydrochloric acid (36.5–38.0%) was ordered from J.T.Baker. Silk fibroin (5% solution) was purchased from Advanced BioMatrix. Sodium nitrite, 6-bromo-1-hexene, chloroplatinic acid hexahydrate (H2PtCl6 ∙ 6H2O), decanoic acid, hydrazine monohydrate, silver acetate, dodecylamine (DDA), lead(II) bromide, methylammonium bromide (MABr), 4-methoxyaniline, sodium hydride, and tetrabutylammonium borohydride (TBAB) were obtained from Sigma-Aldrich. AZ-5214E was used as photoresist (PR). Sodium hydroxide, phenol, and petroleum ether were ordered from Daejung. N,N-dimethylformamide (DMF) was purchased from Honeywell. Silicone elastomer base and silicone elastomer curing agent (Sylgard 184) were obtained from Dow Corning.
8
Hydrosilylation of Nitro Compounds
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All the reagents and solvents were purchased from commercial companies and were used as received without further purification. Poly(methylhydro)siloxanes (PMHS I, 1700–3200 g mol−1), palladium hydroxide on carbon (30 wt% loading), silver acetate (99.0%), 4-nitrophenol (≥99.0%), sodium borohydride (≥99.0%), toluene (anhydrous, 99.8%), tetrahydrofuran (99.9%), ethyl ether (≥99.0%), calcium sulfate (drierite, 8 mesh), triethylsilane (99%) were purchased from Sigma-Aldrich. N-Methyl-aza-2,2,4-trimethylsilacyclopentane was purchased from Gelest. Celite and sodium sulfate (≥99.0%) were purchased from EMD Chemicals. Experiments referring to compounds II and III were performed under N2 protection either in an N2 glovebox or using a Schlenk line.
9
Synthesis of Silver Nanowires
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Silver nitrate (AgNO3), chloroplatinic acid (H2PtCl6·6H2O), poly(vinylpyrrolidone) (PVP), anhydrous ethylene glycol (EG), and sodium chloride (NaCl) were purchased from Sigma Aldrich Chemical (Yongin, Kyeonggi-do, Korea) for the syntheses of AgNWs. A cycloaliphatic-type epoxy resin was purchased from Daicel Chemical Industry (Minato-ku, Tokyo, Japan). The anhydride-type curing agent, hexahydro-4-methylphthalic anhydride, and silver acetate (AgAc) were purchased from Sigma Aldrich Chemical. An imidazole-type catalyst, 1-(2-cyanoethyl)-2-ethyl-4-methylimidazole (imidazole), was purchased from TCI Chemical (Chuo-ku, Tokyo, Japan). The synthesized AgNW was purified using the washing method. Isopropyl alcohol (IPA), purchased from SK Chemical (Seongnam-si, Kyeonggi-do, Korea), was chosen as the solvent because of its low boiling point and capacity as a good stabilizer for AgNWs.
10
Colorimetric CA125 Immunoassay Development
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HAuCl4 (hydrogen tetrachloroaurate (III) hydrate), CTAB (hexadecyltrimethylammonium bromide), NaBH4 (sodium borohydride), ascorbic acid, silver nitrate, NHS (N-hydroxysuccinimide), EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), polysorbate 20, paraformaldehyde, silver acetate, hydroquinone, trypsin, trypan blue, anti-rabbit IgG labelled with alkaline phosphatase, 4-nitrophenyl phosphate bis(cyclohexylammonium) salt, milk powder, human serum, human plasma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit as well as all chemicals for the various buffer solutions were purchased from Sigma Aldrich. Monoclonal mouse antibody anti-CA125 (mAb anti-CA125), polyclonal rabbit antibody anti-CA125 (pAb anti-CA125) and CA125 partial recombinant protein were purchased from Novus Biologicals. Alpha-methoxy-omega-mercapto-poly(ethylene glycol) (mPEG-SH) and alpha-carboxy-omega-mercapto-poly(ethylene glycol) (cPEG-SH), Mw ≈ 5000 gmol−1, were provided by Iris Biotech. All cell culture media, fetal calf serum and antibiotics (penicillin and streptomycin) were purchased from Gibco. All chemicals were of analytical grade. Nitrocellulose membranes with pore size of 0.45 μm were purchased from Whatman.
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