The chow diet (Teklad Mouse/Rat Diet 7002; Harlan Teklad Global Diets)
contains 3.0 kcal/g of metabolizable energy, of which 18% of calories are from
fat, 49% from carbohydrates, and 33% from protein. For high fat diet (HFD)
studies, mice were fed a diet containing 45% calories from fat. The diet was
composed of 24 gm% fat, 41 gm% carbohydrate, and 24 gm% protein (Research Diets;
Cat No. D12451). Total body fat was measured by NMR (Bruker Minispec mq7.5 NMR
analyzer).
contains 3.0 kcal/g of metabolizable energy, of which 18% of calories are from
fat, 49% from carbohydrates, and 33% from protein. For high fat diet (HFD)
studies, mice were fed a diet containing 45% calories from fat. The diet was
composed of 24 gm% fat, 41 gm% carbohydrate, and 24 gm% protein (Research Diets;
Cat No. D12451). Total body fat was measured by NMR (Bruker Minispec mq7.5 NMR
analyzer).