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Neural induction medium

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Neural induction medium is a cell culture medium designed to promote the differentiation of stem cells into neural lineages. It provides the necessary components and growth factors to support the transition of cells towards a neural fate.

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Lab products found in correlation

Mtesr1 medium, by STEMCELL (6 mentions) Accutase, by STEMCELL (3 mentions) Neural progenitor medium, by STEMCELL (3 mentions) Aggrewell 800 plate, by STEMCELL (3 mentions) Matrigel, by Corning (2 mentions) Gentle cell dissociation reagent (gcdr), by STEMCELL (2 mentions) Neuron differentiation and maturation kit, by STEMCELL (2 mentions) Astrocyte differentiation and maturation media, by STEMCELL (2 mentions) Matrigel with reduced growth factors, by Thermo Fisher Scientific (2 mentions) Y 27632, by STEMCELL (1 mentions) Stemdiff neuron differentiation kit, by STEMCELL (1 mentions) H9 wa09 cells, by WiCell (1 mentions) Stemdiff neuron maturation kit, by STEMCELL (1 mentions) Poly l ornithine, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Laminin, by BD (1 mentions) Astrocyte medium, by ScienCell (1 mentions) Accutase, by Merck Group (1 mentions) Stemflex medium, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

STEMdiff Neural Induction Medium (40 citations) STEMdiff™ Neural Induction Medium + SMADi (3 citations)

13 protocols using neural induction medium

1

iPSC-Derived Organoid Differentiation Protocol

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The iPSC line 409b2 was obtained from the Riken Institute in Japan. For routine culture, iPSCs were maintained in mTeSR1 medium (StemCell Technologies) on tissue culture plates coated with Matrigel (Corning) and passaged every 4–5 days using Gentle Cell Dissociation Reagent (StemCell Technologies). For organoid seeding, iPSCs were dissociated with Accutase (StemCell Technologies) and transferred into AggreWell plates in Neural Induction Medium (StemCell Technologies) with 10 µM Y-27632 (StemCell Technologies) at a density of 1000 cells per cavity, following manufacturers’ instructions. Spheroid formation was confirmed visually after 24 hours, and spheroids were maintained in Neural Induction Medium (StemCell Technologies) with daily medium changes. After 5 days, spheroids were harvested from the AggreWell plates and embedded in Matrigel. Medium was changed to neural maintenance medium, a 1:1 mixture of N2- and B27-containing media (N2 medium: DMEM/F12 GlutaMAX, 1 × N2, 5 μg/mL insulin, 1 mM L-glutamine, 1 × non-essential amino acids, 100 μM 2-mercaptoethanol; B27 medium: Neurobasal, 1 × B27, 200 mM L-glutamine) supplemented with 50 U/mL penicillin and 50 mg/mL streptomycin, and exchanged every 2 days. Neural induction in 2D was performed after plating dissociated iPSCs onto Matrigel-coated plates, using the same culture media.
Krieger T.G., Tirier S.M., Park J., Jechow K., Eisemann T., Peterziel H., Angel P., Eils R, & Conrad C. (2020). Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics. Neuro-Oncology, 22(8), 1138-1149.
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2

Efficient Differentiation of iPSCs into NSCs

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Induced pluripotent stem cells colonies were digested with EDTA when the iPSCs reached 70%‐80% confluence and then cultured in an E8 medium for a day. For 2‐7 days, the cells were cultured in a neural induction medium (Stemcell), and NSCs were formed and cultured in an NSC medium (DMEM/F12: Neural basal medium = 1:1 supplemented with 1 × N2, 1 × B27, 20 ng/mL bFGF). Immunofluorescence assay was used to detect the markers Nestin and SOX2.
Zhou L., Li P., Chen N., Dai L., Gao K., Liu Y., Shen L., Wang J., Jiang Y, & Wu Y. (2019). Modeling vanishing white matter disease with patient‐derived induced pluripotent stem cells reveals astrocytic dysfunction. CNS Neuroscience & Therapeutics, 25(6), 759-771.
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3

Efficient hiPSC Neural Induction

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hiPSC colonies were dissociated with trypsin solution (0.05%) and transferred into low attachment six-well plate (CORNING) containing neural induction medium (Stem cell technologies, Cambridge, MA, USA).
Shigyo M., Kobayashi Y., Platoshyn O., Marsala S., Kato Jr T., Takamura N., Yoshida K., Kishino A., Bravo-Hernandez M., Juhas S., Juhasova J., Studenovska H., Proks V., Ciacci J.D, & Marsala M. (2023). Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization. Cell Transplantation, 32, 09636897231163232.
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4

Differentiation of iPSCs into NPCs and Neurons

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The normal human-induced pluripotent stem cells (iPSCs) were obtained from the ATCC (#ACS-1011) and cultured in mTeSR1 medium (StemCell Technologies, #05850). Human iPSCs were differentiated into NPCs using a neural induction medium (StemCell Technologies, #05835), and NPCs were maintained in Neural Progenitor Medium (StemCell Technologies, #05833). An additional NPC line (StemCell Technologies, #70901) was ordered for cell proliferation and neuronal differentiation assays. Neuronal differentiation was induced by a Neuron Differentiation and Maturation Kit (StemCell Technologies, #08500, #08510). For astrocyte differentiation, NPCs were plated on Matrigel-coated culture dish, then incubated in Astrocyte Differentiation and Maturation media (StemCell Technologies, #08540, #08550).
Ding C., Zhang C., Kopp R., Kuney L., Meng Q., Wang L., Xia Y., Jiang Y., Dai R., Min S., Yao W.D., Wong M.L., Ruan H., Liu C, & Chen C. (2020). Transcription factor POU3F2 regulates TRIM8 expression contributing to cellular functions implicated in schizophrenia. Molecular psychiatry, 26(7), 3444-3460.
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5

Directed Neural Differentiation of H9/WA09 Cells

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H9/WA09 cells (WiCell) were grown on Matrigel with reduced growth factors (ThermoFisher Scientific, #354230) in mTeSR1 medium (STEMCELL Technologies, #85850) at 37°C and 5% CO2. Neural lineage differentiation was performed using STEMdiff™ neuron differentiation kit (STEMCELL Technologies, #08500) and STEMdiff™ neuron maturation kit (STEMCELL Technologies, #08510).ES cells were seeded onto AggreWell800 plates (STEMCELL Technologies, #34811) and fed with neural induction medium (STEMCELL Technologies, #05835) to form uni-sized embryoid bodies (EBs). On day 5, EBs were re-plated onto Matrigel-treated 6-well plates. Neural differentiation started from day 11 to day 16. On day 17, cells were treated with Accutase® (STEMCELL Technologies) and seeded and fed with neuronal maturation medium till days 22–24. RNA samples were collected on days 10, 11, and 22 for deep-sequencing analysis. Cells were also seeded onto multi-chamber slides for immunofluorescence (IF) analysis.
Yang X., Xu B., Mulvey B., Evans M., Jordan S., Wang Y.D., Pagala V., Peng J., Fan Y., Patel A, & Peng J.C. (2019). Differentiation of human pluripotent stem cells into neurons or cortical organoids requires transcriptional co-regulation by UTX and 53BP1. Nature neuroscience, 22(3), 362-373.
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6

Astrocyte Differentiation from iPSCs

Cited 1 time
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Astrocytes were differentiated from iPSCs using previously described methods with minor modifications (Li et al. 2015 (link); Roybon et al. 2013 ). Briefly, feeder–free iPS cells were cultured in mTeSR™1 medium (Stemcell Technologies) with 20 ng/ml of bFGF (Life Technologies) on low attachment plates for 5 days. The medium was changed to Neural-induction Medium (Stemcell Technologies) supplemented with 2% B27 (Invitrogen), 1% glutamine (Life Technologies) and 40 ng/ml of bFGF. Media was partially changed every two days for about 10 days. Neurospheres were mechanically dissociated into a single cell suspension and plated onto poly-L-ornithine (Sigma, 20 µg/ml)-laminin coated (BD Biosciences, 10 µg/ml) flasks. Cells were cultured in Astrocyte medium (ScienCell) for 30–45 days (passaged as necessary).
Zhang P.W., Haidet-Phillips A.M., Pham J.T., Lee Y., Huo Y., Tienari P.J., Maragakis N.J., Sattler R, & Rothstein J.D. (2015). Generation of GFAP::GFP astrocyte reporter lines from human adult fibroblast-derived iPS cells using zinc-finger nuclease technology. Glia, 64(1), 63-75.
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7

Differentiation of iPSCs into Astrocytes

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iPSCs were maintained on Matrigel in StemFlex medium (Thermo Fisher Scientific) and passaged every 3–4 days with 0.5 mM EDTA dissociation solution. iPSCs were differentiated into neural progenitor cells (NPC) using an embryoid body (EB) protocol. Briefly, iPSCs at 80% confluence were collected, resuspended in Neural Induction Medium (NIM, StemCell Technologies) and seeded on one well of an Aggrewell 800 plate (StemCell Technologies) at 3 × 106 cells per well. At day five, EBs were seeded on poly-ornithine and laminin (PLO/LAM)-coated dishes in NIM. Rosette selection was performed after 12 days using Rosette Selection Reagent (StemCell Technologies). NPCs were expanded for 7 days in Neural Progenitor Medium (StemCell Technologies). NPCs were then differentiated into astrocyte precursors by seeding dissociated single cells at 1 × 105 cells/cm2 density on PLO/LAM dishes in STEMdiff astrocyte differentiation medium (StemCell Technologies). Astrocyte precursors were maintained for 20 days with medium changes every 48 hr and splitting every week with Accutase (Millipore, Burlington, MA). Astrocytes were expanded for up to 120 days in STEMdiff astrocyte maturation medium (StemCell Technologies).
Battaglia R.A., Beltran A.S., Delic S., Dumitru R., Robinson J.A., Kabiraj P., Herring L.E., Madden V.J., Ravinder N., Willems E., Newman R.A., Quinlan R.A., Goldman J.E., Perng M.D., Inagaki M, & Snider N.T. (2019). Site-specific phosphorylation and caspase cleavage of GFAP are new markers of Alexander disease severity. eLife, 8, e47789.
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8

Generation of iPSC-derived Neural Stem Cells

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NSCs were obtained from the SAFE-IPSc platform at IRMB. Briefly, iPSCs generated from healthy patient using Lentivirus-derived vectors were individualized with Gentle Cell Dissociation Reagent (Stemcell, 07174). They were rinsed out with Dulbecco's modified Eagle's medium/Ham's F12 (DMEM/F-12, Gibco, 31330038) and centrifuged at 300g for 5 min. Dissociated cells were plated on matrigel at a density of 20,000–40,000 cells/cm2 and cultured in neural induction medium (Stemcell, 05835) supplemented with 10 μM ROCK-inhibitor (Y-27632). Cells were allowed to reach 80–90% confluence over 6 days. Medium was changed daily with neural induction medium without Y-27632. iPSC-derived NSCs were passaged by incubation with Trypsin at 0.005% to allow dissociation, and then seeded on poly-D-ornithine/laminin coated plates at 20,000 cells/cm2 in 50% DMEM/F-12 and 50% Neurobasal medium (Thermoscientific) supplemented with 1 × N2 (Thermoscientific), 1 × B27 (Thermoscientific), Glutamax (Thermoscientific) and β-FGF plus EGF (Peprotech, 20 ng/mL each). The medium was changed every two days. Cells were used between passages 5 and 8.
Simonin Y., Loustalot F., Desmetz C., Foulongne V., Constant O., Fournier-Wirth C., Leon F., Molès J.P., Goubaud A., Lemaitre J.M., Maquart M., Leparc-Goffart I., Briant L., Nagot N., Van de Perre P, & Salinas S. (2016). Zika Virus Strains Potentially Display Different Infectious Profiles in Human Neural Cells. EBioMedicine, 12, 161-169.
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9

Neural Differentiation of hESCs

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Monolayer culture protocol was used to initiate neural differentiation. hESCs were plated onto Matrigel-coated 6-well plates at a cell confluence of 2 × 105 cells/cm2, and then cultured in Neural induction medium (Stem Cell Technologies), changing to fresh culture medium every day. The cells were collected for flow cytometry analysis after 3 days.
Liu R., Wubulikasimu Z., Cai R., Meng F., Cui Q., Zhou Y, & Li Y. (2023). NAT10-mediated N4-acetylcytidine mRNA modification regulates self-renewal in human embryonic stem cells. Nucleic Acids Research, 51(16), 8514-8531.
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10

Differentiation of iPSCs into NPCs and Neurons

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The normal human-induced pluripotent stem cells (iPSCs) were obtained from the ATCC (#ACS-1011) and cultured in mTeSR1 medium (StemCell Technologies, #05850). Human iPSCs were differentiated into NPCs using a neural induction medium (StemCell Technologies, #05835), and NPCs were maintained in Neural Progenitor Medium (StemCell Technologies, #05833). An additional NPC line (StemCell Technologies, #70901) was ordered for cell proliferation and neuronal differentiation assays. Neuronal differentiation was induced by a Neuron Differentiation and Maturation Kit (StemCell Technologies, #08500, #08510). For astrocyte differentiation, NPCs were plated on Matrigel-coated culture dish, then incubated in Astrocyte Differentiation and Maturation media (StemCell Technologies, #08540, #08550).
Ding C., Zhang C., Kopp R., Kuney L., Meng Q., Wang L., Xia Y., Jiang Y., Dai R., Min S., Yao W.D., Wong M.L., Ruan H., Liu C, & Chen C. (2020). Transcription factor POU3F2 regulates TRIM8 expression contributing to cellular functions implicated in schizophrenia. Molecular psychiatry, 26(7), 3444-3460.
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